John D. Schuetz, PhD

    John D. Schuetz, PhD

    Member, St. Jude Faculty

    Departments

    Pharmaceutical Sciences

    Contact Information

    John Schuetz, PhD
    Pharmaceutical Sciences
    MS 313, Room D-1052F
    St. Jude Children's Research Hospital
    262 Danny Thomas Place
    Memphis, TN 38105-3678

    Education

    PhD - Medical College of Virginia, Richmond (1984)


    Research Interests


    Selected Publications

    Murphy AJ, Sarrezy V, Wang N, Bijl N, Abramowicz S, Welch CB, Schuetz JD, Yuan-Charvet L. Deficiency of ATP binding cassette transporter B6 in Megakaryocyte progenitors accelerates atheroschlerosis in mice. Arterioscler Thromb Vasc Biol Feb 6, 2014. [Epub ahead of print]

    Haslam IS, Pitre A, Schuetz JD, Paus R. Protection against chemotherapy-induced alopecia: targeting ATP-binding cassette transporters in the hair follicle? Trends Pharmacol Sci Nov;34(11):599-604, 2013.

    Sinha C, Ren A, Arora K, Moon C-S, Sunitha Y, Zhang W, Schuetz JD, Naren AP. Multidrug Resistance Protein 4 (MRP4) mediated regulation of fibroblast cell migration reflects dichotomous role of cyclic nucleotides. J Biol Chem Feb 8;288(6):3786-94, 2013.

    Cheepala S, Hulot J-S, Morgan J, Zhang W, Naren AP, Schuetz JD. Cyclic Nucleotide Compartmentalization: contributions of Phosphodiesterases and ABC transporters. Annu Rev Pharmacol Toxicol 53:231-53, 2013.

    Zhang Y, Li F, Patterson AD, Wang Y, Krausz KW, Neale G, Thomas S, Nachagari D, Vogel P, Vore M, Gonzalez FJ, Schuetz JD. Abcb11 Deficiency Induces Cholestasis Coupled to Impaired β-Fatty Acid Oxidation in Mice. J Biol Chem 287(29):24784-94, 2012.

    Morgan JA, Cheepala SB, Wang Y, Neale G, Adachi M, Nachagari D, Leggas M, Zhao W, Boyd K, Venkataramanan R, Schuetz JD. Deregulated hepatic metabolism exacerbates impaired testosterone production in Mrp4-deficient mice. J Biol Chem 287(18):14456-66, 2012.

    Ulrich DL, Lynch J, Wang Y, Fukuda Y, Nachagari D, Du G, Sun D, Fan Y, Tsurkan L, Potter PM, Rehg JE, Schuetz JD. ATP-dependent mitochondrial porphyrin importer ABCB6 protects against phenylhydrazine toxicity. J Biol Chem 287(16):12679-90, 2012.

    Fukuda Y, Schuetz JD. ABC transporters and their role in nucleoside and nucleotide drug resistance. Biochem Pharmacol 83(8):1073-83, 2012.

    Fukuda Y, Aguilar-Bryan L, Vaxillaire M, Dechaume A, Wang Y, Dean M, Moitra K, Bryan J, Schuetz JD. Conserved intramolecular disulfide bond is critical to trafficking and fate of ATP-binding cassette (ABC) transporters ABCB6 and sulfonylurea receptor 1 (SUR1)/ABCC8. J Biol Chem 286(10):8481-92, 2011.

    Nagai S, Takenaka K, Nachagari D, Rose C, Domoney K, Sun D, Sparreboom A, Schuetz JD. Deoxycytidine kinase modulates the impact of the ABC transporter ABCG2 on clofarabine cytotoxicity. Cancer Res 71(5):1781-91, 2011.

    Takenaka K, Morgan JA, Scheffer GL, Adachi M, Stewart CF, Sun D, Leggas M, Ejendal KFK, Hrycyna CA, Schuetz JD. Substrate overlap between Mrp4 and Abcg2/Bcrp affects purine analogue drug cytotoxicity and tissue distribution. Cancer Res 67:6965-72, 2007.

    Li C, Krishnamurthy PC, Penmatsa H, Marrs KL, Wang XQ, Zaccolo M, Jalink K, Li M, Nelson DJ, Schuetz JD, Naren AP. Spatiotemporal coupling of cAMP transporter to CFTR chloride channel function in the gut epithelia. Cell 131(5):940-51, 2007.

    Krishnamurthy P, Du G, Fukuda Y, Sun D, Mercer K, Wang J, Sosa-Pineda B, Murti G, Schuetz JD. Identification of a mitochondrial porphyrin transporter. Nature 443:586-9, 2006. (2006 Featured in Nature 443 “Making the Paper”; 2008 Selected to Faculty 1000 “must read”)

    Mennone A, Soroka CJ, Cai SY, Harry K, Adachi M, Hagey L, Schuetz JD, Boyer JL. Mrp4-/- mice have an impaired cytoprotective response in obstructive cholestasis. Hepatology 43(5):1013-21, 2006.

    Krishnamurthy P, Ross DD, Nakanishi T, Bailey-Dell K, Zhou S, Mercer KE, Sarkadi B, Sorrentino BP, Schuetz JD. The stem cell marker Bcrp/ABCG2 enhances hypoxic cell survival through interactions with heme.  J Biol Chem 279:24218, 2004.

    Assem M, Schuetz EG, Leggas M, Sun D, Yasuda K, Reid G, Zelcer N, Adachi M, Strom S, Evans RM, Moore DD, Borst P, Schuetz JD. Interactions between hepatic Mrp4 and Sult2A as revealed by the constitutive androstane receptor and Mrp4 knockout mice. J Biol Chem 279:22250, 2004.

    Leggas M, Adachi M, Scheffer GL, Sun D, Wielinga P, Du G, Mercer KE, Zhuang Y, Panetta JC, Johnston B, Scheper RJ, Stewart CF, Schuetz JD. Mrp4 confers resistance to topotecan and acts as a double agent in protecting the brain from chemotherapy. Mol Cell Biol 24(17):7612-21, 2004.

    Zhou S, Morris JJ, Barnes Y, Lan L, Schuetz JD and Sorrentino BP1. Bcrp1 Gene Expression is Required for the Presence of Side Population Stem Cells in Murine Bone Marrow. Proc Natl Acad Sci USA 99:12339-44, 2002.

    Adachi M, Sampath J, Lan L-B, Tammur J, Sun D, Hargrove P, Flatley R,  Tatum A, Allikmets R, Matherly L, Drake R, Schuetz J. MRP4 confers resistance to the cytotoxic effects of the antiviral ganciclovir and plays a role in the bystander effect. J Biol Chem 277(41):38998-9004, 2002.

    Zhou S, Schuetz JD, Bunting KD, Colapietro A-M, Sampath J, Morris JJ, Lagutina I, Grosveld GC, Osawa M, Nakauchi H, Sorrentino BP. The ABC transporter Bcrp1/ABCB2 is expressed in a wide variety of side population stem cells and inhibits hematpoietic development with enforced expression. Nature Med 7:1028-34, 2001.

    Schuetz EG, Yasuda K, Lecureur V, Assem M, Brimer C, Ramchardan V, Strom S, Kim R, Sinal C, Gonzalez F and Schuetz JD. Disrupted Bile Acid Homeostasis Reveals an Unexpected Interaction Among Nuclear Hormone Receptors, Transporters and Cytochorme P450. J Biol Chem 276:39411-8, 2001.

    Sampath J, Sun D, Kidd VJ, Grenet J, Gandhi A, Shapiro LH, Wang Q, Zambetti GP and Schuetz JD. Mutant p53 cooperates with ETS and selectively upregulates MDR1not MRP1. J Biol Chem 276:39359-67, 2001.

    Schuetz EG, Umbenhauer DR, Yasuda K, Brimer C, Nguyen L, Relling MV, Schuetz JD, Schinkel AH. Altered expression of hepatic cytochromes P450 in mice deficient in one or more mdr1 genes. Mol Pharmacol 57:188-197, 2000.

    Schuetz JD, Connelly MC, Sun D, Paibir SG, Flynn PM, Srinivas RV, Kumar A, Fridland A. MRP4: A previously unidentified factor in resistance to nucleoside-based antiviral drugs. Nat Med 5(9):1048-1051, 1999.

    (Commentary in: The Lancet: vol 354:pg 923, 1999; AIDS Alert vol 15(2) pg 24, 2000, BioWorld Today Vol 10 (168) pt 1, 1999, ReutersHealth comments: Gene may contribute to HIV resistance Health eLine 1999-08-30).

    Lutterbach B, Sun D, Schuetz J, Heibert SW. The MYND motif is required for repression of basal transcription from the multidrug resistance 1 promoter by the t(8;21)fusion protein. Mol Cell Biol 18(6):3604-3611, 1998.

    Thottassery JV, Zambetti GP, Arimori K, Schuetz EG, Schuetz JD. p53-dependent Regulation of MDR1 gene expression causes selective resistance to chemotherapeutic agents. Proc Natl Acad Sci 94:11037-11042, 1997.


    Last update: February 2014