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Member, St. Jude Faculty
Investigator, Howard Hughes Medical Institute
Co-Director, Molecular Oncology Program
Structural Biology
Tumor Cell Biology
Brenda Schulman, PhD
Structural Biology
MS 311, Room D-5024E
St. Jude Children's Research Hospital
262 Danny Thomas Place
Memphis, TN 38105-3678
Email: brenda.schulman@stjude.org
Phone: (901) 595-5147
FAX: (901) 595-5785
BA - Johns Hopkins (1989)
PhD - Whitehead Institute/M.I.T. (1996)
Postdoctoral Fellowship - MGH Cancer Center/Harvard Medical School (1996-1998)
Postdoctoral Fellowship - Memorial Sloan-Kettering Cancer Center (1998-2001)
Schulman BA. Twists and turns in ubiquitin-like protein conjugation cascades. Protein Sci 20(12):1941-54, 2011.
Scott DC, Monda JK, Bennett EJ, Harper JW, Schulman BA. N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex. Science September 22, 2011.
Calabrese MF, Scott DC, Duda DM, Grace CR, Kurinov I, Kriwacki RW, Schulman BA. A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases. Nat Struct Mol Biol July 17, 2011.
Duda DM, Scott DC, Calabrese MF, Zimmerman ES, Zheng N, Schulman BA. Structural regulation of cullin-RING ubiquitin ligase complexes. Curr Opin Struct Biol January 31, 2011.
Wang J, Taherbhoy AM, Hunt HW, Seyedin SN, Miller DW, Miller DJ, Huang DT, Schulman BA. Crystal structure of Uba2(ufd)-Ubc9: insights into E1-E2 interactions in Sumo pathways. PLoS One 5(12):e15805, 2010.
Zimmerman ES, Schulman BA, Zheng N. Structural assembly of cullin-RING ubiquitin ligase complexes. Curr Opin Struct Biol 20(6):714-721, 2010.
Jubelin G, Taieb F, Duda DM, Hsu Y, Samba-Louaka A, Nobe R, Penary M, Watrin C, Nougayrede JP, Schulman BA, Stebbins CE, Oswald E. Pathogenic bacteria target NEDD8-conjugated cullins to hijack host-cell signaling pathways. PLoS Pathog 6(9): 1128, 2010.
Scott DC, Monda JK, Grace CR, Duda DM, Kriwacki RW, Kurz T, Schulman BA. A Dual E3 mechanism for Rub1 ligation to Cdc53. Mol Cell 39(5): 784-796, 2010.
Schulman BA, Haas AL. Structural Biology: Transformative Encounters. Nature 463: 889-90, 2010.
Reed D, Shen Y, Shelat AA, Arnold A, Ferreira AM, Zhu F, Mills N, Smithson DC, Regni CA, Bashford D, Cicero SA, Schulman BA, Jochemsen AG, Guy RK, Dyer MA. Identification and characterization of the first small-molecule inhibitor of MDMX. Journal of Biological Chemistry 285: 10786-96, 2010.
Kamadurai H*, Souphron J*, Scott DC, Duda DM, Miller DJ, Stringer D, Piper RC, Schulman BA. Insights into ubiquitin transfer cascades from a structure of a UbcH5B~Ubiquitin-HECTNEDD4L complex. Molecular Cell 36: 1095-102, 2009.
Siergiejuk E, Scott DC, Schulman BA, Hofmann K, Kurz T, Peter M. Cullin neddylation and substrate-adaptors counteract SCF inhibition by the CAND1-like protein Lag2 in Saccharomyces cerevisiae. The EMBO Journal 28: 3845-56, 2009.
Zhuang M*, Calabrese MF*, Liu J, Waddell MB, Nourse A, Hammel M, Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, White KP, Schulman BA. Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases. Molecular Cell 36: 39-50, 2009.
Wang J, Dye BT, Rajashankar KR, Kurinov I, Schulman BA. Insights into Anaphase Promoting Complex TPR subdomain assembly from a CDC26-APC6 structure. Nature Structural and Molecular Biology 16: 987-989, 2009.
Wang J, Schulman BA. (G2)Bringing an E2 to E3. Structure 17: 916-917, 2009.
Regni CA, Roush RF, Miller D, Nourse A, Walsh CT, Schulman BA. How the MccB bacterial ancestor of ubiquitin E1 initiates biosynthesis of the Microcin C7 antibiotic. The EMBO Journal 28: 1953-1964, 2009.
Schulman BA*, Harper JW*. Ubiquitin-like protein activation by E1 enzymes: the apex for downstream signalling pathways. Nature Reviews: Molecular Cell Biology 10: 319-331, 2009. *equal contributors
Huang DT*, Ayrault O*, Hunt HW, Taherbhoy A, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel, MF1, Schulman BA1. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Molecular Cell 33: 483-495, 2009. (Cover story; Preview, Structure 17: 321-322)
Duda DM, Borg LA, Scott DC, Hunt HW, Hammel M, Schulman BA. Structural Insights into NEDD8 Activation of Cullin-RING Ligases: Conformational Control of Conjugation.Cell 134: 995-1006, 2008. (Preview, Cell 135: 209-211)
Souphron J, Waddell MB, Paydar A, Tokgöz-Gromley Z, Roussel MF, Schulman BA. Structural dissection of a gating mechanism preventing misactivation of ubiquitin by NEDD8’s E1. Biochemistry 47: 8961-8969, 2008.
Huang DT, Zhuang M, Ayrault O, Schulman BA. Identification of Conjugation Specificity Determinants Allows Unmasking Vestigial Preference for Ubiquitin within the NEDD8 E2. Nature Structural and Molecular Biology 15: 280-287, 2008.
Dye BT, Schulman BA. Structural Mechanisms Underlying Posttranslational Modification by Ubiquitin-Like Proteins.Annual Reviews of Biophysics and Biomolecular Structure 36: 131-150, 2007.
Duda DM, van Waardenburg R, Borg LA, McGarity S, Nourse A, Waddell MB, Bjornsti JA, Schulman BA. Structure of a SUMO-binding-motif mimic bound to Smt3p-Ubc9p: conservation of a noncovalent Ubiquitin-like protein-E2 complex as a platform for selective interactions within a SUMO pathway. Journal of Molecular Biology 369: 619-630, 2007.
Huang DT, Hunt HW, Zhuang M, Ohi MD, Holton JM, Schulman BA. Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity. Nature 445:394-398, 2007. (News and Views, Nature 445: 375-376. 2007; Research Highlights, Nature Reviews Molecular Cell Biology 8: 178-179, 2007)
Van Waardenburg RCAM, Duda, D, Lancaster CS, Schulman BA, Bjornsti MA. Distinct Functional Domains of Ubc9 Dictate Cell Survival and Resistance to Genotoxic Stress. Molecular and Cellular Biology 26:4958-4969, 2006.
Harper, JW, Schulman BA. Structural Complexity in Ubiquitin Recognition. Cell 124:1133-1136, 2006.
Shalizi A, Gaudilliere B, Yuan Z, Stegmuller J, Shirogane T, Ge Q, Tan Y, Schulman B, Harper JW, Bonni A. A calcium-regulated MEF2 sumoylation switch controls postsynaptic differentiation. Science 311:1012-1017, 2006.
Schulman BA and Chen ZJ. Protein ubiquitination: CHIPping away the symmetry. Molecular Cell 20: 653-655, 2005.
Eletr ZM*, Huang DT*, Duda DM, Schulman BA, Kuhlman B. E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer. Nature Structural and Molecular Biology 12: 933-934, 2005.
Hao B, Zheng N, Schulman BA, Wu G, Miller JJ, Pagano M, Pavletich NP. Structural Basis of the Cks1-Dependent Recognition of p27(Kip1) by the SCF(Skp2) Ubiquitin Ligase. Molecular Cell 20: 9-19, 2005.
Huang DT, Schulman BA. Expression, Purification and Characterization of the E1 for Human NEDD8, the Heterodimeric APPBP1-UBA3 Complex. Methods in Enzymology 398: 9-20, 2005.
Li T, Pavletich NP, Schulman BA, Zheng N. High-Level Expression and Purification of Recombinant SCF Ubiquitin Ligases. Methods in Enzymology 398: 125-142, 2005.
Duda DM and Schulman BA. Tag-Team SUMO Wrestling. Molecular Cell 18: 612-614, 2005.
Duda DM, Walden H, Sfondouris J, Schulman BA. Structural Analysis of Escherichia Coli ThiF. Journal of Molecular Biology 349: 774-86, 2005.
Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA. Structural Basis for Recruitment of Ubc12 by an E2 Binding Domain in NEDD8's E1. Molecular Cell 17:341-50, 2005. (Preview, Molecular Cell 17:474-475, 2005).
Macauley MS, Errington WJ, Okon M, Scharpf M, Mackereth CD, Schulman BA, McIntosh LP. Structural and dynamic independence of isopeptide-linked RanGAP1 and SUMO-1. Journal of Biological Chemistry 279:49131-49137, 2004.
Huang DT, Miller DW, Mathew R, Cassell R, Holton JM, Roussel MF, Schulman BA. A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8. Nature Structural and Molecular Biology 11:927-935, 2004. (News and Views, Nature Structural and Molecular Biology 11: 908-909)
Zhao C, Beaudenon SL, Kelley ML, Waddell MB, Yuan W, Schulman BA, Huibregtse JM, Krug RM. The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an interferon a/b-induced ubiquitin-like protein. Proc Natl Acad Sci 101:7578-7582, 2004.
Huang DT, Walden H, Duda D, Schulman BA. Ubiquitin-like protein activation. Oncogene 23: 1958-71, 2004.
Walden H, Podgorski MS, Huang DT, Miller DW, Howard RJ, Minor DL Jr, Holton JM, Schulman BA. The Structure of the APPBP1-UBA3-NEDD8-ATP Complex Reveals the Basis for Selective Ubiquitin-like Protein Activation by an E1. Molecular Cell 12: 1427-1437, 2003.
Wu G, Xu G, Schulman BA, Jeffrey PD, Harper JW and Pavletich NP. Structure of a ß-TRCP-Skp1-ß-Catenin Complex: Destruction Motif Binding and Lysine Specificity of the SCFß-TRCP Ubiquitin Ligase. Molecular Cell 11: 1445-1456, 2003.
Walden H, Podgorski MS, Schulman BA. Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8. Nature 422: 330-334, 2003. (News and Views, Nature Structural Biology 10: 244-246)
Staropoli, JF, McDermott, C., Martinat, C., Schulman, B., Demireva, E., and Abeliovich, A. Parkin is a Component of an SCF-like Ubiquitin Ligase Complex and Protects Postmitotic Neurons from Kainate Excitotoxicity. Neuron 37: 735-749, 2003.
Bencsath KP, Podgorski MS, Pagala VR, Slaughter CA, Schulman BA. Identification of a multifunctional binding site on Ubc9p required for Smt3p conjugation. Journal of Biological Chemistry 277:47938-47945, 2002.
Kile BT, Schulman BA, Alexander WS, Nicola NA, Hilton DJ. Opening Pandora’s SOCS box – a tale of destruction and degradation. Trends in Biochemical Sciences 27:235-241, 2002.
Zheng N, Schulman BA, Song L, Miller JJ, Jeffrey PD, Wang P, Chu C, Koepp DM, Elledge SJ, Pagano M, Conaway JW, Harper JW, Pavletich NP. Structure of the Cul 1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex. Nature 416:703-709, 2002.
Ganiatsas S, Dow R, Thompson A, Schulman B, Germain D. A splice variant of Skp2 is retained in the cytoplasm and fails to direct cyclin D1 ubiquitination in the uterine cancer cell line SK-UT. Oncogene 20:3641-3650, 2001.
Latres E, Chiarle R, Schulman BA, Pavletich NP, Pellicer A, Inghirami G, Pagano M. Role of the F-box protein Skp2 in lymphomagenesis. Proceedings of the National Academy of Sciences 96:2515-2520, 2001.
Schulman BA, Carrano AC, Jeffrey PD, Bowen Z, Kinnucan ERE, Finnin MS, Elledge SJ, Harper JW, Pagano M, Paveletich NP. Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. Nature 408:381-386, 2000.
Last update: December 2011
