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When the Pediatric Cancer Genome Project (PCGP) was launched in 2010, the literature contained only one publication describing the analysis of a cancer genome by using next-generation sequencing (NGS) technology. Analyzing and validating the sequences of the unprecedented 600 pairs of tumor and matched non-tumor DNA was considered the greatest challenge in the PCGP.
With the establishment of the Department of Computational Biology and the PCGP Validation Laboratory in 2010, investigators at St. Jude have been able to conduct intense research and development of NGS technologies. Over the course of the 3-year project and in collaboration with the High-Performance Computing Facility and Department of Information Sciences, these efforts have resulted in innovative computational methods, an automated data analysis pipeline, an integrative genomic database, and the capacity for high-throughput experimental validation and recurrence screening.