Light Microscopy
The CTIC assists and trains St. Jude scientists in all aspects of imaging research including experimental design, execution of the experimental plan, image analysis, quantification, presentation and publication. The instrumentation in the CTIC supports nearly every imaging modality imaginable, from the simplest to the most complex. Listed below are many of the techniques we routinely employ:
- Confocal laser scanning microscopy (CLSM)
- Spinning disk confocal microscopy
- Total internal reflection fluorescence (TIRF)
- Multiphoton imaging
- Förster resonance energy transfer (FRET)
- Frequency domain fluorescence lifetime imaging (FLIM)
- Fluorescence recovery after photobleaching (FRAP)
- Live cell imaging with environment control
- Long term time-lapse imaging
- Calcium imaging
- Microinjection
- Brightfield and darkfield imaging
- Phase contrast and differential interference contrast (DIC)
- Widefield fluorescence and deconvolution
- Image tiling of large specimens
- Fluorescence correlation spectroscopy (FCS)
- Single molecule imaging
- Superresolution techniques including PALM and STORM