Junmin Peng, PhD

Junmin Peng, PhD

Associate Member, St. Jude Faculty
Director, St. Jude Proteomics Facility


Structural Biology
Developmental Neurobiology

Contact Information

Junmin Peng, PhD
Structural Biology
MS 311, Room D1034C
St. Jude Children's Research Hospital
262 Danny Thomas Place
Memphis, TN 38105-3678
Email: junmin.peng@stjude.org
Phone: (901) 595-7499
FAX: (901) 595-3032


BS – Wuhan University, Wuhan, China
PhD – University of Iowa
Postdoctoral Fellowship - Harvard Medical School

Research Interests

Selected Publications

Xu P, Tan H, Duong DM, Yang Y, Kupsco J, Moberg KH, Li H, Jin P, Peng J. Stable Isotope Labeling with Amino Acids in Drosophila for Quantifying Proteins and Modifications. J Proteome Res July 25, 2012. [Epub ahead of print]

Xie L, Pi X, Mishra A, Fong G, Peng J, Patterson C. PHD3-dependent hydroxylation of HCLK2 promotes the DNA damage response. J Clin Invest Jul 16, 2012. [Epub ahead of print]

Seyfried NT, Gozal YM, Donovan LE, Herskowitz JH, Dammer EB, Xia Q, Ku L, Chang J, Duong DM, Rees HD, Cooper DS, Glass JD, Gearing M, Tansey MG, Lah JJ, Feng Y, Levey A, Peng J. Quantitative analysis of the detergent-insoluble brain proteome in frontotemporal lobar degeneration using SILAC internal standards. J Proteome Res 11:2721-38, 2012.

Felberbaum R, Wilson NR, Cheng D, Peng J, Hochstrasser M. Desumoylation of the ER Membrane VAP Protein Scs2 by Ulp1 and SUMO Regulation of the Inositol Synthesis Pathway. Mol Cell Biol 32:64-75, 2012.

Scaglione KM, Zavodszky E, Todi SV, Patury S, Xu P, Rodríguez-Lebrón E, Fischer S, Konen J, Djarmati A, Peng J, Gestwicki JE, Paulson HL Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP. Mol Cell 43:599-612, 2011.

Dammer EB, Na CH, Xu P, Seyfried NT, Duong DM, Cheng D, Gearing M, Rees H, Lah JJ, Levey AI, Rush J, Peng J. Polyubiquitin linkage profiles in three models of proteolytic stress suggest etiology of Alzheimer disease. J Biol Chem 286:10457-65, 2011.

Franco M, Seyfried NT, Brand AH, Peng J (co-corresponding author), Mayor U. A novel strategy to isolate ubiquitin conjugates reveals wide role of ubiquitination during neural development Mol Cell Proteomics 10:M110.002188, 2011.

Bingol B, Wang CF, Arnott D, Cheng D, Peng J, Sheng M. Autophosphorylated CaMKIIalpha acts as a scaffold to recruit proteasomes to dendritic spines. Cell 140:567-578, 2010.

Seyfried NT, Gozal YM, Dammer EB, Xia Q, Duong DM, Cheng D, Lah JJ, Levey AI, Peng J. Multiplex SILAC analysis of a cellular TDP-43 proteinopathy model reveals protein inclusions associated with SUMOylation and diverse polyUb chains. Mol Cell Proteomics. 9:705-18, 2010.

Zhou JY, Afjehi-Sadat L, Asress S, Duong DM, Cudkowicz M, Glass JD, Peng J. Galectin-3 is a candidate biomarker for ALS: Discovery by a proteomics approach. J Proteome Res 9:5133-41, 2010.

Xu P, Duong DM, Seyfried NT, Cheng D, Xie Y, Robert J, Rush J, Hochstrasser M, Finley D, Peng J. Quantitative proteomics reveals the function of unconventional ubiquitin chains in proteasomal degradation. Cell 137:133-145, 2009.

Xu P, Duong DM, Xia Q, Peng J. Systematical optimization of reverse-phase chromatography for protein identification. J Proteome Res 8:3944-50, 2009.

Liu Y, Ye X, Jiang F, Liang C, Cheng D, Peng J, Kinch L, Grishin N, Liu Q. C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation. Science 325:750-753, 2009.

Edbauer D, Cheng D, Batterton MN, Wang CF, Duong DM, Yaffe MB, Peng J (co-corresponding author), Sheng M. Identification and characterization of neuronal MAP kinase substrates using a specific phosphomotif antibody. Mol Cell Proteomics 8:681-98, 2009.

Gozal YM, Duong DM, Gearing M, Cheng D, Hanfelt J, Funderburk C, Peng J (co-corresponding author), Lah JJ, Levey AI. Proteomics analysis reveals novel components in the detergent-insoluble subproteome in Alzheimer’s disease. J Proteome Res 8:5069-79, 2009.

Qi HH, Ongusaha PP, Myllyharju J, Cheng D, Pakkanen O, Shi Y, Lee SW, Peng J, Shi Y. Prolyl 4-hydroxylation regulates Argonaute 2 stability. Nature 455:421-4, 2008.

Xia Q, Cheng D, Duong DM, Gearing M, Lah JJ, Levey AI, Peng J. Phosphoproteomic analysis of human brain by calcium phosphate precipitation and mass spectrometry. J Proteome Res 7:2845-51, 2008.

Xu P, Peng J. Characterization of polyubiquitin chain structure by middle-down mass spectrometry. Anal Chem 80:3438-44, 2008.

Seyfried N, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J. A systematic approach to validating ubiquitinated proteome. Anal Chem 80:4161-9, 2008.

Choe EA, Liao L, Zhou, JY, Cheng D, Duong DM, Jin P, Tsai LH, Peng J. Neuronal morphogenesis is regulated by the interplay between cyclin-dependent kinase 5 and the ubiquitin ligase mind bomb 1. J Neurosci 27:9503-9512, 2007.

Cheng D, Hoogenraad CC, Rush J, Ramm E, Schlager MA, Duong DM, Xu P, Wijayawardana SR, Hanfelt J, Nakagawa T, Sheng M, Peng J.Relative and absolute quantification of postsynaptic density proteome isolated from rat forebrain and cerebellum. Mol Cell Proteomics 5:1158-1170, 2006.

Wang M, Cheng D, Peng J, Pickart CM. Molecular determinants of polyubiquitin linkage selection by a HECT ubiquitin ligase. EMBO J 25:1710-1719, 2006.

Liao L, Cheng D, Wang J, Duong DM, Losik TG, Gearing M, Rees HD, Lah JJ, Levey AI, Peng J. Proteomic characterization of postmortem amyloid plaques isolated by laser capture microdissection. J Biol Chem 279:37061-37068, 2004.

Peng J (corresponding author), Kim MJ, Cheng D, Duong DM, Gygi SP, Sheng M. Semiquantitative proteomic analysis of rat forebrain postsynaptic density fractions by mass spectrometry. J Biol Chem 279:21003-21011, 2004.

Peng J, Elias JE, Schwartz D, Thoreen CC, Cheng D, Marshiscky G, Roelofs J, Finley D, and Gygi SP. A proteomics approach to protein ubiquitination. Nat Biotechnol 21:921-926, 2003.

Peng J, Elias JE, Thoreen CC, Licklider LJ, Gygi SP. Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for large-scale protein analysis: the yeast proteome. J Proteome Res 2:43-50, 2003.

Peng J Gygi SP. Proteomics: the move to mixtures (review). J Mass Spectrom 36:1083-1091, 2001.

Lee MS, Kwon YT, Li M, Peng J, Friedlander RM, Tsai LH. Neurotoxicity induces calpain cleavage of p35 to p25. Nature 405:360-4, 2000.

Peng J, Marshall N, and Price DH. Identification of a cyclin subunit required for the function of Drosophila P-TEFb. J Biol Chem 273:13855-60, 1998.

Peng J, Zhu Y, Milton J, Price DH. Identification of multiple cyclin subunits of human P-TEFb. Genes Dev 12:755-62, 1998.

Last update: July 2012