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Erin Schuetz, PhD

Erin G. Schuetz, PhD

Member, St. Jude Faculty

Departments

Pharmaceutical Sciences

Contact Information

Erin Schuetz, PhD
Pharmaceutical Sciences
MS 313, Room I5303
St. Jude Children's Research Hospital
262 Danny Thomas Place
Memphis, TN 38105-3678
Email: erin.schuetz@stjude.org
Phone: (901) 595-2205
FAX: (901) 525-3125

Education

PhD - Medical College of Virginia, Richmond


Research Interests

  • Regulation of cytochrome P4503A
  • Interplay of drug transport, cytochromes P450 and nuclear hormone receptors
  • Pharmacogenetics of drug transporters, cytochromes P450 and nuclear hormone receptors

The influence of Pqp on CYP3A regulation


Selected Publications

Lin YS, Yasuda K, Assem M, Cline C, Barber J, Li CW, Kholodovych V, Ai N, Chen JD, Welsh WJ, Elkins S, Schuetz EG. The major human pregnane X receptor (PXR) splice variant, PXR.2, exhibits significantly diminished ligand-activated transcriptional regulation. Drug Metab Dispos Jun;37(6):1295-304, 2009.

Schadt EE, Molony C, Chudin E, Hao K, Yang X, Lum PY, Kasarskis A, Zhang B, Wang S, Suver C, Zhu J, Millstein J, Sieberts S, Lamb J, GuhaThakurta D, Derry J, Storey J, Mehrabian M, Drake TA, Lusis AJ, Smith RC, Guengerich FP, Strom SC, Schuetz E, Rushmore TH, Ulrich R. Mapping the genetic architecture of gene expression in human liver. PLoS Biol 6(5):e107, 2008. PMCID: PMC2365981.

Poonkuzhali B, Lamba J, Strom S, Sparreboom A, Thummel K, Watkins P, Schuetz E. Association of breast cancer resistance protein/ABCG2 phenotypes and novel promoter and intron 1 single nucleotide polymorphisms. Drug Metab Dispos 36:780-95, 2008.

Lamba J, Lamba V, Strom S, Venkataramanan, Schuetz E. Novel SNPs in the Promoter and Intron 1 of Human PXR/NR1I2 and Their Association with CYP3A4 Expression. Drug Metab Dispos 36:169-81, 2008.

Marzolini C, Tirona RG, Gervasini G, Poonkuzhali B, Assem M, Lee W, Leake BF, Schuetz JD, Schuetz EG, Kim RB. A Common Polymorphism in the Bile Acid Receptor FXR is Associated with Decreased Hepatic Target Gene Expression. Mol Endocrinol 21(8):1769-1780, 2007.

Zhou C, Assem M, Tay JC, Watkins PB, Blumberg B, Schuetz EG, Thummel KE. Steroid and xenobiotic receptor and vitamin D receptor crosstalk mediates CYP24 expression and drug-induced osteomalacia. J Clin Invest 116(6):1703-1712, 2006.

Finkelstein D, Lamba V, Assem M, Rengelshausen J, Yasuda K, Strom S, Schuetz E. The ADME transcriptome in Hispanic versus White donor livers: Evidence of a globally enhanced NR1I3 (CAR, Constitutive Androstane Receptor) gene signature in Hispanics. Xenobiotica 36:989-1012, 2006.

Lamba J, Strom S, Venkataramanan R, Thummel KE, Lin YS, Liu W, Cheng C, Lamba V, Watkins P, Schuetz E. MDR1 genotype is associated with Hepatic CYP3A4 basal and induction phenotype. Clin Pharmacol Therap 79(4):325-338, 2006.

Lamba J, Chen X, Lan L, Kim JW, Relling MV, Kazuto Y, Strom S, Sun D, Schuetz JD, Schuetz EG. Increased CYP3A4 Copy Number in TONG/HCC cells but not in DNA from Other Humans. Pharmacogenetics & Genomics 16(6):415-427, 2006.

Xu Y, Hashizume T, Shuhart M, Davis CL, Sakaki T, Kalhorn, TF, Watkins PB, Schuetz EG, Thummel K. CYP3A4 catalyzes the 23- and 24-hydroxylation of 1a,25-dihydroxyvitamin D3: Implications for drug induced osteomalacia. Mol Pharm 69(1):56-65, 2006.

Mouly SJ, Matheny C, Smith G, Lamba J, Lamba V, Paine MF, Pusek SN, Schuetz EG, Stewart PW, Watkins PB. Variation in oral clearance of saquinavir is predicted by CYP3A5*1 genotype but not by enterocyte content of CYP3A5. Clin Pharmacol Therapeutics 78:605-618, 2005.

Krasowski MD, Yasuda K, Hagey LR, Schuetz EG. Evolutionary selection across the nuclear hormone receptor superfamily with a focus on the NR1I subfamily (vitamin D, pregnane X, and constitutive androstane receptors). Nuclear Receptor Sep 30;3:2-20, 2005.

Dinakarpandian D, Raheja V, Mehta S, Schuetz E, Rogan PK. Tandem Machine Learning for the Identification of Genes Regulated by Transcription Factors. BMC Bioinformatics 2005, 6:204-210, 2005.

Assem M, Schuetz EG, Leggas M, Sun D, Yasuda K, Reid G, Zelcer N, Adachi M, Strom S, Evans RM, Moore DD, Borst P, Schuetz JD. Interactions between hepatic MRP4 and Sult2A as revealed by the constitutive androstane receptor and Mrp4 knockout mice. J Biol Chem 279(21):22250-22257, 2004.

Ramirez J, Innocenti F, Schuetz EG, Flockhart DA, Relling V, Santucci R, Ratain MJ. CYP2B6, CYP3A4, and CYP2C19 are responsible for the in vitro N-demethylation of meperidine in human liver microsomes. Drug Metab Dispos 32:930-936, 2004.

Lamba V, Yasuda K, Lamba J, Brimer Cline C, Assem M, Lin Y, Davila J, Strom S, Schuetz EG. PXR (NR1I2): Splice Variants in Human Tissues, including Brain, and identification of neurosteroids and nicotine as PXR activators. Tox Appl Pharmacol 199: 251-265, 2004.

Lamba V, Lamba J, Yasuda K, Strom S, Davila J, Hancock M, Fackenthal JD, Rogan P, Ring B, Wrighton S, Schuetz EG. Hepatic CYP2B6 expression: gender and population differences and relationship to CYP2B6 genotype and CAR expression. J Pharmacol Exp Ther 307:906-922, 2003.

Tirona RG, Lee W, Leake BF, Lan L, Brimer C, Lamba V, Parviz F, Duncan SA, Inoue Y, Gonzalez FJ, Schuetz EG, Kim RB. The orphan nuclear receptor HNF4α determines PXR- and CAR-mediated xenobiotic induction of CYP3A4. Nat Med 9:220-224, 2003.

Zamber CP, Lamba JK, Yasuda K, Farnum J, Thummel K, Schuetz JD, Schuetz EG. Natural allelic variants of BCRP and their relationship to BCRP expression. Pharmacogenetics 13:19-28, 2003.

Hebert MF, Dowling LS, Gierwatowski C, Lin YS, Edwards KL, David CL, Marsh CL, Schuetz EG, Thummel KE. Association between ABCB1 genotype and post-liver transplantation renal dysfunction in patients receiving calcineurin inhibitors. Pharmacogenetics 13:661-674, 2003.

Givens RC, Lin YS, Dowling ALS, Thummel KE, Lamba JK, Schuetz EG, Stewart PW, Watkins PB. CYP3A5 genotype predicts renal CYP3A activity and blood pressure in healthy adults. J Appl Physiol 95:1297-1300, 2003.

Schuetz EG, Lan L, Kim R, Kocarek T, Schuetz J, Strom S. Development of a Real-time in vivo Transcription Assay: Application Reveals PXR Mediated Induction of CYP3A4 by Cancer Chemotherapeutic Agents. Mol Pharmacol 62:1-7, 2002.

Ekins S, Mirny L, Schuetz E. A ligand-based approach to understanding selectivity of nuclear receptors PXR, CAR, FXR, LXRα and LXRβ. Pharm Res 19:1788-1800, 2002.

Schuetz EG, Strom S, Yasuda K, Lecureur V, Assem M, Lamba J, Kim, RB, Ramachandran V, Komoroski BJ, Venkataramanan R, Cai H, Sinal C, Gonzalez FJ, Schuetz JD. Disrupted bile acid homeostasis reveals an unexpected interaction among nuclear hormone receptors, transporters and cytochrome P450. J Biol Chem 276:39411-39418, 2001.

Schuetz E, Strom S. Promiscuous regulator of xenobiotic removal. Nat Med 7:536-537, 2001.

Thummel KE, Brimer C, Yasuda K, Thottassery J, Senn T, Lin Y, Ishizuka H, Kharasch E, Schuetz J, Schuetz E. Transcriptional control of intestinal CYP3A4 by 1a,25-dihydroxy vitamin D3. Mol Pharm 60:1-8, 2001.

Kuehl P, Zhang J, Lin Y, Lamba J, Assem M, Schuetz J, Watkins P, Maurel P, Daly A, Wrighton S, Hall SD, Relling M, Schuetz J, Brimer C, Yasuda K, Strom S, Thummel K, Boguski MS, Schuetz E. Sequence Diversity in CYP3A promoters and characterization of the genetic basis for polymorphic CYP3A5 expression. Nat Genet 27:383-391, 2001.

Schuetz EG, Umbenhauer DR, Yasuda K, Brimer C, Nguyen L, Relling MV, Schuetz JD, Schinkel AH. Altered expression of hepatic cytochromes P450 in mice deficient in one or more mdr1 genes. Mol Pharmacol 57:188-197, 2000.

Lan L-B, Dalton JT, Schuetz EG. Mdr1 limits CYP3A metabolism in vivo. Mol Pharmacol 58:8863-8869, 2000.

Schuetz EG, Brimer C, Schuetz J. Environment xenobiotics and the antihormones cyproterone acetate and spironolactone use the nuclear hormone pregnane X receptor (PXR) to activate the CYP4503A23 hormone response element. Mol Pharmacol 54:1113-1117, 1998.

Schuetz EG, Schinkel AH, Relling MV, Schuetz JD. P-glycoprotein: a major determinant of rifampicin-inducible expression of cytochrome P4503A in mice and humans. Proc Natl Acad Sci 93:4001-4005, 1996.

Schuetz EG, Beck WT, Schuetz JD. Modulators and substrates of P-glycoprotein and cytochrome P4503A coordinately up-regulate these proteins in human colon carcinoma cells. Mol Pharmacol 49:63-72, 1996.

Watkins PB, Wrighton SA, Schuetz EG, Molowa DT, Guzelian PS. Identification of glucocorticoid-inducible cytochrome P-450 in the intestinal mucosa of rats and man. J Clin Invest 80:1029-1036, 1987.

Molowa DT, Schuetz EG, Wrighton SA, Watkins PB, Kremers P, Mendez-Picon G, Parker GA, Guzelian PS. Complete cDNA sequence of a cytochrome P-450 inducible by glucocorticoids in human liver. Proc Natl Acad Sci 83:5311-5315, 1986.

Schuetz EG, Wrighton SA, Safe SH, Guzelian PS. Regulation of cytochrome P-450 by phenobarbital and phenobarbital-like inducers in adult rat hepatocytes in primary monolayer culture and in vivo. Biochemistry 25:1124-1133, 1986.

Schuetz EG, Guzelian PS. Induction of cytochrome P-450 by glucocorticoids in rat liver. II. Evidence that glucocorticoids regulate induction of cytochrome P-450 by a non-classical receptor mechanism. J Biol Chem 259:2007-2012, 1984.

Last update: June 2009

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