James Ihle, PhD

James N. Ihle, PhD

Member, St. Jude Faculty
Chair, Biochemistry
Edward F. Barry Endowed Chair in Biochemistry
Co-Leader, Cancer Genetics, Biochemistry and Cell Biology Program

Departments

Biochemistry

Contact Information

James Ihle, PhD
Biochemistry
MS 340, Room D4007D
St. Jude Children's Research Hospital
262 Danny Thomas Place
Memphis, TN 38105-3678
Email: james.ihle@stjude.org
Phone: (901) 595-3422
FAX: (901) 525-8025

Education

BS - Iowa State University, Ames
MS - University of Georgia, Athens
PhD - University of Georgia, Athens


Research Interests

The biochemical basis by which various cytokines bring about their biological responses


Selected Publications

Pelletier S, Gingras S, Howell S, Vogel P, Ihle JN. An early onset progressive motor neuron disorder in Scyl1-deficient mice is associated with mislocalization of TDP-43. J Neurosci Nov 21;32(47):16560-73, 2012. doi: 10.1523/JNEUROSCI.1787-12.2012. PMID: 23175812

Rawlings JS, Gatzka M, Thomas PG, Ihle JN. Chromatin condensation via the condensing II complex is required for peripheral T-cell quiescence. EMBO J 30(2):263-276, 2011.

Takahasi Y, Takahashi, M, Carpino, N, Jou ST, Chao, JR, Tanaka S, Shigeyoshi Y, Parganas E, Ihle JN. Leukemia inhibitory factor regulates trophoblast giant cell differentiation via JAK1-Stat3-SOCS3 pathway. Mol Endocrinol 22(7):1673-1681, 2008.

Chao JR, Parganas E, Boyd K, Hong CY, Opferman JT, Ihle JN. Hax-1 mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons. Nature 452(7183):98-102, 2008.

Funakoshi-Tago M, Pelletier S, Moritake H, Parganas E, Ihle JN. Jak2 FERM domain interaction with the erythropoietin receptor regulates Jck2 kinase activity. Mol Cell Biol 28(5):1792-801, 2007.

Gingras S, Pelletier S, Boyd K, Ihle JN. Characterization of a family of novel cysteine-serine-rich nuclear proteins (CSRNP). PLoS One 2(8):e808, 2007.

Ihle JN, Gilliland DG. Jak2: normal funcation and role in hematopoietic disorders. Curr Opin Genet Dev 17(1):8-14, 2007.

Takahashi Y, Dominici M, Swift J, Nagy C, Ihle JN. Trophoblast stem cells rescue placental defect in SOCS3-deficient mice. J Biol Chem 281, 11444-11445, 2006.

Pelletier S, Gingras S, Funakoshi-Tago M, Howell S, Ihle JN. Two Domains of the Erythropoietin Receptor are Sufficient for Jak2 Binding/Activation and Function. Mol Cell Biol 26(22):8527-8538, 2006.

Gatzka M, Piekorz R, Moriggl R, Rawlings J, Ihle JN. A role for STAT5A/B in protection of peripheral T-lymphocytes from postactivation apoptosis: insights from gene expression profiling. Cytokine 34, 143-154, 2006.

Funakoshi-Tago M, Pelletier S, Matsuda T, Parganas E, Ihle JN. Receptor specific downregulation of cytokine signaling by autophosphorylation in the FERM domain of Jak2. EMBO J 25, 4763-4772, 2006.

Zhang J, Randall MS, Loyd MR, Li W, Schweers RL, Persons DA, Rehg, JE, Noguchi CT, Ihle JN, Ney PA.  Role of erythropoietin receptor signaling in Friend virus-induced erythroblastosis and polycythemia. Blood 107:73-78, 2006.

Qasimi P, Ming-Lum A, Ghanipour A, Ong CJ, Cox ME, Ihle JN, Cacalano N, Yoshimura A, Mui AL. Divergent mechanisms utilized by SOCS3 to mediate interleukin-10 inhibition of tumour necrosis factor alpha and nitric oxide production by macrophages. J Biol Chem 281:6316-6324, 2006.

Bertolino E, Reddy K, Medina KL, Parganas E, Ihle JN, Singh H. Regulation of IL-7 dependent immunoglobulin VH gene rearrangements by Stat5. Nat Immunol 6:836-843, 2005.

Mead PE, Parganas E, Ohtsuka S, Morishita K, Gamer L, Kuliyev E, Wright CV, Ihle JN. Evi-1 expression in xenopus. Gene Expr Patterns 5:601-608, 2005.

Piekorz RP, Gingras S, Hoffmeyer A, Ihle JN, Weinstein Y. Regulation of progesterone levels during preganancy and parturition by signal transducer and activator of transcription 5 and 20 α-hydroxysteroid dehydrogenase. Mol Endocrinol 19(2):431-440, 2005.

Moriggl R, Sexl V, Kenner L, Duntsch C, Stangl K, Gingras S, Hoffmeyer A, Bauer A, Piekorz R, Wang D, Bunting KD, Wagner EF, Sonneck K, Valent P, Ihle JN, Beug H. Stat5 tetramer formation is associated eith leukemogenesis. Cancer Cell 7(1):87-99, 2005.

Gingras S, Parganas E, de Pauw A, Ihle JN, Murray PJ. Re-examination of the role of suppressor of cytokine signaling 1 (SOCS1) in the regulation of toll-like receptor signaling. J Biol Chem 279(52):54702-54707, 2004.

Matsuda T, Feng J, Witthuhn, BA, Sekine Y, Ihle JN. Determination of the transphosphorylation sites of Jak2 kinase. Biochem Biophys Res Commun 325(2):586-594, 2004.

Schuendeln MM, Piekorz RP, Wichmann C, Lee Y, McKinnon PJ, Boyd K, Takahashi Y, Ihle JN. The centrosomal, putative tumor suppressor ptrotein TACC2 is dispensable for normal development and deficiency does not lead to cancer. Mol Cell Biol 24, 6403-6409, 2004.

Carpino N, Turner S, Mekala D, Takahashi Y, Zang H, Geiger TL, Doherty P, Ihle JN. Regulation of ZAP-70 activation and TCR signaling by two related proteins, Sts-1 and Sts-2. Immunity 20(1):37-46, 2004.

Carpino N, Thierfelder WE, Chang MS, Saris C, Turner SJ, Ziegler SF, Ihle JN. Absence of an essential role for thymic stromal lymphopoietin receptor in murine B-cell development. Mol Cell Biol 24(6):2584-92, 2004.

Lang R, Pauleau AL, Parganas E, Takahashi Y, Mages J, Ihle JN, Rutschman R, Murray PJ. SOCS3 regulates the plasticity of gp130 signaling. Nat Immunol 4, 546-550, 2003.

Sexl V, Kovacic B, Piekorz R, Moriggl R, Stoiber D, Hoffmeyer A, Liebminger R, Kudlacek O, Weisz E, Rothammer K, Ihle JN. Jak1 deficiency leads to enhanced Abelson-induced B-cell tumor formation. Blood 101: 4937-4943, 2003.

Takahashi Y, Carpino N, Cross JC, Torres M, Parganas E, Ihle JN. SOCS3: an essential regulator of LIF receptor signaling in trophoblast giant cell differentiation. EMBO J 22: 372-384, 2003.

Jeffers JR, Parganas E, Lee Y, Yang C, Wang J, Brennan J, Maclean KH, Han J, Chittenden T, Ihle JN, McKinnon PJ, Cleveland JL, and Zambetti GP. Puma is an essential mediator of p53-dependent and –independent apoptotic pathways. Cancer Cell 4:321-328, 2003.

Baudino TA, McKay C, Pendeville-Samain H, Nilsson JA, Maclean KH, White EL, Davis AC, Ihle JN, Cleveland JL. C-Myc is essential for vasculogenesis and angiogenesis during development and tumor progression. Genes Dev 16: 2530-2543, 2002.

Jou ST, Carpino N, Takahashi Y, Piekorz R, Chao JR, Carpino N, Wang D, Ihle JN. An essential, non-redundant role for the phosphoinosite 3-kinase p110δ in signaling by the B cell receptor complex. Mol Cell Biol 22: 8580-8591, 2002.

Carpino N, Kobayashi R, Zang H, Takahashi Y, Jou ST, Feng J, Nakajima H, Ihle JN. Identification, cDNA cloning, and targeted deletion of p70, a novel, ubiquitously expressed SH3 domain-containing protein. Mol Cell Biol 22: 7491-7500, 2002.

Zhao S, Zoller K, Masuko M, Rojnuckarin P, Yang XO, Parganas E, Kaushansky K, Ihle JN, Papayannopoulou T, Willerford DM, Clackson T, Blau CA. JAK2, complemented by a second signal from c-kit or flt-3, triggers extensive self-renewal of primary multipotential hematopoietic cells. EMBO J 21:2159-2167, 2002.

Liao HJ, Kume T, McKay C, Xu MJ, Ihle JN, Carpenter G. Absence of erythrogenesis and vasculogenesis in Plcg1-deficient mice. J Biol Chem 277:9335-9341, 2002.

Piekorz RP, Hoffmeyer A, Duntsch CD, McKay C, Nakajima H, Sexl V, Snyder L, Rehg J, Ihle JN. The centrosomal protein TACC3 is essential for hematopoietic stem cell function and genetically interfaces with p53 regulated apoptosis. EMBO J 21:653-664, 2002.

Last update: February 2013