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Flow Cytometry and Cell Sorting Shared Resource

Richard A. Ashmun, PhD, Director
The Flow Cytometry and Cell Sorting Shared Resource provides investigators with access to state-of-the-art equipment and expertise in all aspects of flow cytometry and cell sorting for the analysis and separation of cell populations stained with fluorescent compounds. The facility is available to all investigators on a first-come, first-served basis.

The facility's instrumentation includes eleven BD Biosciences flow cytometers, including seven benchtop flow cytometer analyzers (two 4-color FACS Caliburs and five 18-color LSR IIs), four high-speed FACS Aria cell sorters, and two Miltenyi Biotec AutoMACS magnetic cell separators. Experienced personnel operate all of the instruments; investigators may obtain access to run any of three flow cytometry analyzers on an individual basis. Three experienced senior technologists are available to perform sample preparation and staining when required, and we provide a wide variety of monoclonal antibody reagents and fluorescent dyes for cell labeling.

The facility is equipped with computer workstations that can be used by investigators for detailed analysis of flow cytometry data. The facility's director and assistant director are available for consultation on experimental design, and interpretation of data and flow cytometry results.