Dario Vignali, PhD

Dario A. A. Vignali, PhD

Member, St. Jude Faculty
Vice Chair, Immunology

Departments

Immunology

Contact Information

Dario A Vignali, PhD
Immunology
MS 351, Room E7054
St. Jude Children's Research Hospital
262 Danny Thomas Place
Memphis, TN 38105-3678
Email: dario.vignali@stjude.org
Phone: (901) 595-2332
FAX: (901) 595-3107

Education

PhD - London School of Hygiene & Tropical Medicine, University of London


Research Interests

Various aspects of T cell function:

  • T cell receptor (TCR):CD3 complex [molecular initiation and control of signal transduction; control of TCR transport and downmodulation; role of CD3 in T cell development and function].
  • Regulatory T cells [identification of novel Treg molecules; mechanism of Treg function; control of Treg function by LAG-3 and IL-35; role of LAG-3 in pDC function].
  • Type 1 diabetes [importance of TCR specificity and affinity in the diabetogenic or regulatory potential of T cells; mechanisms that regulate T cell islet entry and diabetogenicity; tolerance induction by manipulation of ß cells].

Vignali's Research Group


Selected Publications

To request reprints and reagents: vignali.lab@stjude.org

Collison LW, Pillai MR, Chaturvedi V, Vignali DAA. Regulatory T cell suppression is potentiated by target T cells in a cell contact, IL-35- and IL-10-dependent manner. J Immunol 182:6121-6128, 2009.

Szymczak-Workman AL, Vignali, DAA. Cutting Edge: Regulatory T cells do not require stimulation through their T cell receptor to suppress. J Immunol 182:5188-5192, 2009.

Workman CJ, Wang Y, El Kasma KC, Pardoll DM, Murray PJ, Drake CG, Vignali, DAA. LAG-3 negatively regulates plasmacytoid dendritic cell homeostasis. J Immunol 182:1885-91, 2009.

Blackburn SD, Shin H, Haining WN, Workman CJ, Polley A, Betts MR, Freeman GJ, Vignali DAA, Wherry EJ.Co-regulation of CD8 T cell exhaustion during chronic viral infection by multiple inhibitory receptors. Nature Immunology 10:29-37, 2009.

Wang H, Matsuzawa A, Brown S, Zhou J-R, Guy CS, Tseng P-H, Forbes K, Nicholson TP, Sheppard PW, Haecker H, Karin M, Vignali DAA. Analysis of non-degradative protein ubiquitylation with a monoclonal antibody specific for lysine 63-linked polyubiquitin. PNAS 105:20197-20202, 2008.

Vallabhapurapu S, Matsuzawa A, Zhang WZ, Tseng P-H, Keats JJ, Wang H, Vignali DAA, Bergsagel PL, Karin M. TRAF2 and TRAF3 carry out non-redundant and complementary functions to activate NIK-dependent alternative NF-κB signaling. Nature Immunology 9:1364-1370, 2008.

Collison LW, Vignali DAA. Interleukin-35: odd one out or part of the family? Immunological Reviews 226:248–262, 2008.

Matsuzawa A, Tseng P-H, Vallabhapurapu S, Luo J-L, Zhang W, Wang H, Vignali DAA, Gallagher E, Karin K. Essential cytoplasmic translocation of a cytokine receptor-assembled signaling complex. Science 321:663-8, 2008.

Vignali DAA, Collison LW, Workman CJ. How regulatory T cells work. Nature Reviews Immunology 8:523-532, 2008.

Holst J, Wang H, Durick Eder K, Workman CJ, Boyd K, Baquet Z, Singh H, Forbes K, Chruscinski A, Smeyne R, van Oers NSC, Utz PJ, Vignali DAA. T cell receptor:CD3 ITAM-mediated scalable signaling ensures effective negative selection and prevents autoimmunity. Nature Immunology 9:658-666, 2008. 

Burton AR, Vincent E, Arnold PY, Lennon, G, Smeltzer M, Li C-S, Haskins K, Hutton J, Tisch RM, Sercarz EE, Santamaria P, Workman CJ, Vignali DAA.  On the pathogenicity of autoantigen-specific T cell receptors. Diabetes 57: 1321-1330, 2008.

Vignali DAA. How many mechanisms do regulatory T cells need? Eur J Immunol 38: 901-937, 2008. [Invited Review for EJI in Honor of Rolf Zinkernagel]

Collison LW, Workman CJ, Kuo TK, Boyd K, Wang Y, Vignali K, Cross R, Sehy D, Blumberg RS, Vignali DAA. The inhibitory cytokine IL-35 contributes to regulatory T cell function.  Nature 450: 566-569, 2007.

Li N, Wang Y, Forbes K, Vignali KM, Heale BS, Saftig P, Hartmann D, Black R, Rossi JJ, Blobal C, Dempsey PJ, Workman CJ, Vignali DAA. Metalloproteases regulate T cell proliferation and effector function via LAG-3. EMBOJ 26: 494-504, 2007.

Li N, Boyd K, Dempsey PJ, Vignali DAA. Non-cell autonomous expression of TNF-α-converting enzyme ADAM17 is required for normal lymphocyte development. J Immunol 178: 4214-4221, 2007.

Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DAA. Generation of T cell receptor retrogenic mice. Nature Protocols 1:406-417, 2006.

Holst J, Vignali KM, Burton AR, Vignali DAA. Rapid analysis of T cell selection and function in vivo using T cell receptor retrogenic mice. Nat Methods 3:191-197, 2006.

Szymczak A, Workman CJ, Gill D, Dilioglou S, Vignali KM, Palmer E, Vignali DAA. The CD3ε proline-rich sequence, and its interaction Nck, is not required for T cell development and function. J Immunol 175:270-275, 2005.

Szymczak A, Vignali DAA. Plasticity and rigidity in AP-2-mediated internalization of the T cell receptor: CD3 complex. J Immunol 174:4153-4160, 2005.

Workman CJ, Vignali DAA. Negative regulation of T cell homeostasis by LAG-3 (CD223). J Immunol 174:688-695, 2005.

Li N, Workman CJ, Martin SM, Vignali DAA. Biochemical analysis of the regulatory T cell protein Lymphocyte Activation Gene-3 (LAG-3; CD223). J Immunol 173:6806-6812, 2004.

Huang C-T, Workman CJ, Flies D, Pan X, Marson AL, Zhou G, Hipkiss EL, Ravi S, Kowalski J, Lavitsky HI, Powell JD, Pardoll DM, Drake CG, Vignali DAA. Role of LAG-3 in regulatory T cells. Immunity 21:503-13, 2004. [Issue Cover]

Arnold PY, Royer A, Vignali DAA. Diabetes incidence is unaltered in GAD-65-specific TCR retrogenic NOD mice: generation by retroviral-mediated stem cell gene transfer. J Immunol 173:3103-3111, 2004.

Szymczak A, Workman CJ, Wang Y, Vignali KM, Dilioglou S, Vanin E, Vignali DAA. Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nature Biotechnology 22:589-594, 2004.

Workman CJ, Cauley LS, Kim I-J, Blackman M, Woodland DL, Vignali DAA. LAG-3 (CD223) regulates the size of the expanding T cell population following antigen activation in vivo. J Immunol 172:5450-5455, 2004.

La Gruta NL, Liu HY, Rhodes M, Wiest D, Vignali DAA. Architectural changes in the T cell receptor: CD3 complex induced by MHC: peptide ligation. J Immunol 172:3662-3669, 2004.

Clatza A, Bonifaz LC, Vignali DAA, Moreno J. CD40-induced aggregation of MHC class II and CD80 on the cell surface leads to an early enhancement in antigen presentation. J Immunol 171:6655-6658, 2003.

Workman CJ, Vignali DAA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 33:970-979, 2003.

Cauley LS, Cookenham T, Miller T, Adams PS, Vignali KM, Vignali DAA, Woodland DL. Cutting Edge: Virus-specific CD4+ memory T cells in nonlymphoid tissues express a highly activated phenotype. J Immunol 169:6655-6658, 2002.

Arnold PY, Vignali KM, Miller T, La Gruta NL, Cauley LS, Haynes L, Adams PS, Swain S, Woodland DL, Vignali DAA. Reliable generation and use of MHC class II:γ2aFc multimers for the identification of antigen-specific CD4+ T cells. J Immunol Methods 271:137-151, 2002.

Workman CJ, Dugger KJ, Vignali DAA. Cutting Edge: Molecular analysis of the negative regulatory function of LAG-3. J Immunol 169:5392-5395, 2002.

Workman CJ, Rice D, Dugger KJ, Kurschner C, Vignali DAA. Phenotypic and kinetic analysis of the murine CD4-related glycoprotein, CD223 (LAG-3). Eur J Immunol 32:2255-2263, 2002.

Arnold PY, La Gruta NL, Miller TB, Vignali KM, Adams PS, Woodland DL, Vignali DAA. The majority of immunogenic epitopes generate CD4+ T cells that are dependent on MHC class II-bound peptide flanking residues. J Immunol 169:739-749, 2002.

Liu HY, Rhodes M, Wiest D, Vignali DAA. On the dynamics of TCR:CD3 complex cell surface expression and downmodulation. Immunity 13:665-675, 2000. [Issue Cover]

Last update: June 2009

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