Sharon Frase

Sharon Frase

  • Director of Electron Microscopy

Research Interests

Mrs. Frase received her initial training in histology and electron microscopy at St. Jude Children’s Research Hospital and during her 35 year career has established shared resource facilities at the University of Tennessee Health Science Center and the University of Memphis. Her laboratory focus is on establishing standard procedures for immunogold techniques and TEM tomography analysis. She is also interested in developing partnerships with other institutions to allow St Jude researchers access to new technology.

Education

Memphis State University, Memphis, TN
Certified Electron Microscopy Technologist, Microscopy Society of America
ASCP Registered Histologist

Selected Publications

Corbin DR, Rehg JE, Shepherd DL, Stoilov P, Percifield RJ, Horner L, Frase S, Zhang YM, Rock CO, Hollander JM, Jackowski S, Leonardi R. Excess coenzyme A reduces skeletal muscle performance and strength in mice overexpressing human PANK2. Mol Genet Metab Feb 2, 2017.

Fukuda Y, Cheong PL, Lynch J, Brighton C, Frase S, Kargas V, Rampersaud E, Wang Y, Sankaran VG, Yu B, Ney PA, Weiss MJ, Vogel P, Bond PJ, Ford RC, Trent RJ, Schuetz JD. The severity of hereditary porphyria is modulated by the porphyrin exporter and Lan antigen ABCB6. Nat Commun 7:12353, 2016.

Diouf B, Devaraju P, Janke LJ, Fan Y, Frase S, Eddins D, Peters JL, Kim J, Pei D, Cheng C, Zakharenko SS, Evans WE. Msh2 deficiency leads to dysmyelination of the corpus callosum, impaired locomotion, and altered sensory function in mice. Sci Rep 6:30757, 2016.

Campos Y, Qiu X, Gomero E, Wakefield R, Horner L, Brutkowski W, Han YG, Solecki D, Frase S, Bongiovanni A, d'Azzo A. Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier. Nat Commun 7:11876, 2016.

Liu Z, Man SM, Zhu Q, Vogel P, Frase S, Fukui Y, Kanneganti TD. DOCK2 confers immunity and intestinal colonization resistance to Citrobacter rodentium infection. Sci Rep p 27814, 2016.

Man SM, Karki R, Sasai M, Place DE, Kesavardhana S, Temirov J, Frase S, Zhu Q, Malireddi RK, Kuriakose T, Peters JL, Neale G, Brown SA, Yamamoto M, Kanneganti TD. IRGB10 liberates bacterial ligands for sensing by the AIM2 and caspase-11-NLRP3 inflammasomes. Cell 167(2):382-396.e17, 2016.

Quarato G, Guy CS, Grace CR, Llambi F, Nourse A, Rodriguez DA, Wakefield R, Frase S, Moldoveanu T, Green DR. Sequential Engagement of Distinct MLKL Phosphatidylinositol-Binding Sites Executes Necroptosis. Mol Cell 61(4):589-601, 2016.

Milasta S, Dillon CP, Sturm OE, Verbist KC, Brewer TL, Quarato G, Brown SA, Frase S, Janke LJ, Perry SS, Thomas PG, Green DR. Apoptosis-Inducing-Factor-Dependent Mitochondrial Function Is Required for T Cell but Not B Cell Function. Immunity 44(1):88-102, 2016.

Morgan JA, Lynch J, Panetta JC, Wang Y, Frase S, Bao J, Zheng J, Opferman JT, Janke L, Green DM, Chemaitilly W, Schuetz JD. Apoptosome activation, an important molecular instigator in 6-mercaptopurine induced Leydig cell death. Sci Rep 5:16488, 2015.

Cheepala SB, Pitre A, Fukuda Y, Takenaka K, Zhang Y, Wang Y, Frase S, Pestina T, Gartner TK, Jackson C, Schuetz JD. The ABCC4 membrane transporter modulates platelet aggregation. Blood 126(20):2307-2319, 2015.

Yamashita T, Hakizimana P, Wu S, Hassan A, Jacob S, Temirov J, Fang J, Mellado-Lagarde M, Gursky R, Horner L, Leibiger B, Leijon S, Centonze VE, Berggren PO, Frase S, Auer M, Brownell WE, Fridberger A, Zuo J. Outer hair cell lateral wall structure constrains the mobility of plasma membrane proteins. PLoS Genet 11(9):e100500, 2015.

Hiler D, Chen X, Hazen J, Kupriyanov S, Carroll PA, Qu C, Xu B, Johnson D, Griffiths L, Frase S, Rodriguez AR, Martin G, Zhang J, Jeon J, Fan Y, Finkelstein D, Eisenman RN, Baldwin K, Dyer MA. Quantification of retinogenesis in 3D cultures reveals epigenetic memory and higher efficiency in iPSCs derived from rod photoreceptors. Cell Stem Cell 7(1): 101-105, 2015.

Wang X, Bathina M, Lynch J, Koss B, Calabrese C, Frase S, Schuetz JD, Rehg JE, Opferman JT. Deletion of MCL-1 causes lethal cardiac failure and mitochondrial dysfunction. Genes Dev 27(12):1341-1364, 2013.

Stoner TD, Krauss S, Turner JC, Seiler P, Negovetich NJ, Stallknecht DE, Frase S, Govorkova EA, Webster RG. Susceptibility of avian influenza viruses of the N6 subtype to the neuraminidase inhibitor oseltamivir. Antiviral Res 93(3):322-329, 2012.

Zhang Y, Li F, Wang Y, Pitre A, Fang ZZ, Frank MW, Calabrese C, Krausz KW, Neale G, Frase S, Vogel P, Rock CO, Gonzalez FJ, Schuetz JD. Maternal bile acid transporter deficiency promotes neonatal demise. Nat Commun 6:8186, 2015.

Martins RA, Davis D, Kerekes R, Zhang J, Bayazitov IT, Hiler D, Karakaya M, Frase S, Gleason S, Zakharenko SS, Johnson DA, Dyer MA. Retinoblastoma (Rb) regulates laminar dendritic arbor reorganization in retinal horizontal neurons. Proc Natl Acad Sci U S A 108(52):21111-21116, 2011.

Wilson MW, Jackson JS, Phillips BX, Buchanan J, Frase S, Wang F, Steinle JJ, Stewart CF, Mandrell TD, Haik BG, Williams JS. Real-time ophthalmoscopic findings of superselective intraophthalmic artery chemotherapy in a nonhuman primate model. Arch Ophthalmol 129(11):1458-1465, 2011.

Teitz T, Stanke JJ, Federico S, Bradley CL, Brennan R, Zhang J, Johnson MD, Sedlacik J, Inoue M, Zhang ZM, Frase S, Rehg JE, Hillenbrand CM, Finkelstein D, Calabrese C, Dyer MA, Lahti JM. Preclinical models for neuroblastoma: establishing a baseline for treatment. PLoS One 6(4):1-16, 2011.

Ajioka I, Martins RA, Bayazitov IT, Donovan S, Johnson DA, Frase S, Cicero SA, Boyd K, Zakharenko SS, Dyer MA. Differentiated horizontal interneurons clonally expand to form metastatic retinoblastoma in mice. Cell 131(2):378-901, 2007.

Johnson DA, Zhang J, Frase S, Wilson M, Rodriguez-Galindo C, Dyer MA. Neuronal Differentiation and synaptogenesis in retinoblastoma. Cancer res 67(6):2701-11, 2007. 

Song L, Ying Y, Xiangying C, Mitchell S, Shuangying L, Songmai M, Gallagher JW, Shelness GS, Mahan J, Frase S, Mansbach CM, Weinberg RB, Black DD. Overexpression of apolipoprotein A-IV enhances lipid secretion in IPEC-1 cells by increasing chylomicron size. Dec. 2005. eprint

Fitzgerald MEC, Tolley E, Frase S, Zagvazdin Y, Miller RF, Hodos W, Reiner A.  Functional and morphological assessment of age-related changes in the choroidal and outer retina in pigeons. VisNeurosci 18:1-19, 2001.

Lamoreaux WJ, Fitzgerald MEC, Needham, GR, Frase S, Bayakly N, Coons LB.  Localization of nitric oxide synthase in the salivary gland dusts of feeding female dermacentor variabilis (Say) and the effect of endogenous nitric oxide on isolated salivary gland ducts. Microscopy & Microanalysis (Midroscopy Society of America ) Cleveland, OH, 1997.

Fitzgerald MEC, Frase S, Miller RF, Hodos W, Reiner A. Decrease in the abundance of choroidal vessels is correlated with loss of visual acuity in aged pigeons. Invest Ophthalmol Vis Sci (Suppl) 35(4):S553, 1995.

Fitzgerald MEC, Frase S, Hodos W, Reiner A. Outer retinal and choroidal pathology in aged pigeons. Invest Ophthalmol Vis Sci (Suppl.) 35(4):1952, 1994.

Judge D, Payant J , Frase S, Wood TO. Anterior stromal micropuncture electron microscopic changes in the rabbit cornea. Cornea 9(2): 152-160, 1990.

Last update: February 2017