Victoria Centonze Frohlich, PhD

Victoria Centonze Frohlich, PhD

  • Director of Light Microscopy


BS – Rensselaer Polytechnic Institute, Troy, New York
PhD – Dartmouth College, Hanover, New Hampshire

Research Interests

I am formally trained as a Cell Biologist and throughout my career I have applied a variety of both light and electron microscopy techniques to my research. My experience with light microscopy includes phase-contrast and Nomarski-DIC transmitted light imaging techniques, and wide-field, confocal and multiphoton fluorescence imaging techniques. In addition, I have extensively used FRAP (Fluorescence Recovery after Photobleaching), FRET (Fluorescence Resonance Energy Transfer), FLIM (Fluorescence Lifetime Imaging Microscopy), photolysis and ion imaging as well as intravital imaging and whole animal imaging.  In addition, I have more than 20 years of experience managing centralized core facilities at the University of Wisconsin at Madison, the University of Texas Health Science Center at San Antonio, and at St. Jude Children’s Research Hospital.

Selected Publications

Churchman ML, Low J, Qu C, Paietta EM, Kasper LH, Chang Y, Payne-Turner D, Althoff MJ, Song G, Chen SC, Ma J, Rusch M, McGoldrick D, Edmonson M, Gupta P, Wang YD, Caufield W, Freeman B, Li L, Panetta JC, Baker S, Yang YL, Roberts KG, McCastlain K, Iacobucci I, Peters JL, Centonze VE, Notta F, Dobson SM, Zandi S, Dick JE, Janke L, Peng J, Kodali K, Pagala V, Min J, Mayasundari A, Williams RT, Willman CL, Rowe J, Luger S, Dickins RA, Guy RK, Chen T, Mullighan CG. Efficacy of Retinoids in IKZF1-Mutated BCR-ABL1 Acute Lymphoblastic Leukemia. Cancer Cell 28(3):343-356, 2015.

Yamashita T, Hakizimana P, Wu S, Hassan A, Jacob S, Temirov J, Fang J, Mellado-Lagarde M, Gursky R, Horner L, Leibiger B, Leijon S, Centonze VE, Berggren PO, Frase S, Auer M, Brownell WE, Fridberger A, Zuo J. Outer Hair Cell Lateral Wall Structure Constrains the Mobility of Plasma Membrane Proteins. PLoS Genet 11(9):e1005500, 2015.

Di Cairano ES, Davalli AM, Perego L, Sala S, Sacchi VF, La Rosa S, Finzi G, Placidi C, Capella C, Conti P, Centonze VE, Casiraghi F, Bertuzzi F, Folli F, Perego C. The glial glutamate transporter 1 (GLT1) is expressed by pancreatic beta-cells and prevents glutamate-induced beta-cell death. J Biol Chem Apr 22;286(16):14007-14018, 2011. PMID: 21335552

Zheng X, Goins BA, Cameron IL, Santoyo C, Bao A, Frohlich VC, Fullerton GD. Ultrasound-guided intratumoral of collagenase-2 improved liposome drug accumulation in solid tumor xenografts. Cancer Chemother Pharmacol Jan; 67(1):173-82, 2011. PMID: 20306263

Chavez AO, Kamath S, Jani R, Sharma LK, Monroy A, Abdul-Ghani MA, Centonze VE, Sathyanarayana P, Coletta DK, Jenkinson CP, Bai Y, Folli F, Defronzo RA, Tripathy D. Effect of Short-Term Free Fatty Acids Elevation on Mitochondrial Function in Skeletal Muscle of Healthy Individuals. J Clin Endocrinol Metab Oct 28, 2009. Epub PMC2805487

Sabbah A, Chang TH, Harnack R, Frohlich V, Tominaga K, Dube PH, Xiang Y, Bose S. Activation of innate immune antiviral responses by Nod2. Nat Immunol Oct; 10(10):1073-80, 2009. PMID:19701189COVER ART for journal issue

Centonze VE, Pawley JB. Tutorial on practical confocal microscopy and use of the confocal test specimen (Revised) In: Ed. J. Pawley. The Handbook of Biological Confocal Microscopy 3rd Edition. New York: Plenum Press; 2006.

Sims P, Albrecht R, Pawley JB, Centonze V, Deerinck T, Hardin J. When light microscope resolution is not enough: Correlational light microscope and electron microscope In: Sims P, Albrecht R, Pawley JB, CentonzeV, Deerinck T, Hardin J. Handbook of Biological Confocal Microscopy 3rd Edition. New York: Plenum; 2006.

Firulli BA, Krawchuk D, Centonze VE, Vargesson N, Virshup DM, Conway SJ, Cserjesi P, Laufer E, Firulli AB. Altered Twist1 and Hand2 dimerization is associated with Saethre-Chotzen syndrome and limb abnormalities. Nat Genet Apr; 37(4):373-381, 2005. PMC2568820

Centonze VE, Sun M, Masuda A, Gerritsen H, Herman B. Fluorescence energy transfer imaging microscopy. Methods Enzymol 360: 542-560, 2003. PMID: 12622167

Krishnan RV, Masuda A, Centonze VE, Herman B. Quantitative imaging of protein-protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera. J Biomed Opt Jul; 8(3):362-367, 2003. PMID: 12880340

Krishnan RV, Saitoh H, Terada H, Centonze VE, Herman B. Development of a multiphoton fluorescence lifetime imaging microscopy (FLIM) system using a Streak Camera. Review of Scientific Instrumentation 74:2714-2721, 2003.

Centonze VE. Introduction to multiphoton excitation imaging for the biological sciences. Methods Cell Biol 70:129-48, 2002. PMID: 12512323

Wang W, Wyckoff JB, Frohlich VC, Oleynikov Y, Hüttelmaier S, Zavadil J, Cermak L, Bottinger EP, Singer RH, White JG, Segall JE, Condeelis JS. Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. Cancer Res Nov; 62(21):6278-6288, 2002. PMID: 12414658

Hockberger PE, Skimina TA, Centonze VE, Lavin C, Chu S, Dadras S, Reddy JK, White JG. Activation of flavin-containing oxidases underlies light-induced production of H2O2 in mammalian cells.Proc Natl Acad Sci USA May; 96(11):6255-6260, 1999. PMID: 10339574

Centonze VE, White JG. Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophys J Oct; 75(4):2015-2024, 1998. PMID:9746543

Wokosin DV, Centonze VE, Crittenden S, White J. Three-photon-excitation flourescence imaging of biological specimens using an all-solid-state laser. Bioimaging 4:208-214, 1997.

Centonze VE, Chen Y, Severson TF, Borisy GG, Nibert ML. Visualization of individual reovirus particles by low-temperature high-resolution scanning electron microscopy. J Structural Biol 115:215-225, 1995. PMID: 8573466

Chen Y, Centonze VE, Verkhovsky A, Borisy GG. Imaging of cytoskeletal elements by low-temperature high-resolution scanning electron microscopy. J Microsc Jul; 179(Pt1):67-76, 1995. PMID: 7666412

Centonze VE, Borisy GG. Application and limitations of photobleaching and fluorescence imaging In: eds. B. Herman and K. Jacobson. Optical Microscopy for Biology. New York: Wiley-Liss, Inc; 1990.

Last update: September 2015