Byoung Ryu, PhD
Byoung Ryu, PhD

Byoung Ryu, PhD

Associate Member, St. Jude Faculty

  • Director, St. Jude Vector Development Core

Departments

Divisions

Education

PhD - University of Tennessee Health Science Center, Memphis, Tennessee
MS - Konkuk University, Seoul, Korea
BS - Konkuk University, Seoul, Korea

Honors & Awards

  • 2004-2007 Sumara Endowed Fellowship for Transplantation and Gene Therapy

Research Interests

  • Stem cell gene therapy for blood disorders focusing on sickle cell disease
  • Cancer immunotherapy with chimeric antigen receptor (CAR) T cells
  • Genome editing for cell and gene therapy
  • Viral vector design and development including lentiviral and AAV vectors
  • Process development for scale-up vector manufacturing 

Selected Publications

Negre O, Bartholomae C, Beuzard Y, Cavazzana M, Christiansen L, Courne C, Deichmann A, Denaro M, de Dreuzy E, Finer M, Fronza R, Gillet-Legrand B, Joubert C, Kutner R, Leboulch P, Maouche L, Paulard A, Pierciey FJ, Rothe M, Ryu B, Schmidt M, von Kalle C, Payen E, Veres G. Preclinical evaluation of efficacy and safety of an improved lentiviral vector for the treatment of β-thalassemia and sickle cell disease. Curr Gene Ther 15(1):64-81, 2015.

Certo MT, Gwiazda KS, Kuhar R, Sather B, Curinga G, Mandt T, Brault M, Lambert AR, Baxter SK, Jacoby K, Ryu BY, Kiem HP, Gouble A, Paques F, Rawlings DJ, Scharenberg AM. Coupling endonucleases with DNA end-processing enzymes to drive gene disruption. Nat Methods 9(10):973-5, 2012.

Astrakhan A, Sather BD, Ryu BY, Khim S, Singh S, Humblet-Baron S, Ochs HD, Miao CH, Rawlings DJ. Ubiquitous high-level gene expression in hematopoietic lineages provides effective lentiviral gene therapy of murine Wiskott-Aldrich Syndrome. Blood 119(19):4395-407, 2012.

Andrews SF, Dai X, Ryu BY, Gulick T, Ramachandran B, Rawlings DJ. Developmentally regulated expression of MEF2C limits the response to BCR engagement in transitional B cells. Eur J Immunol 42(5):1327-36, 2012.

Munoz NM, Beard BC, Ryu BY, Luche RM, Trobridge GD, Rawlings DJ, Scharenberg AM, Kiem HP. Novel reporter systems for facile evaluation of I-SceI-mediated genome editing. Nucleic Acids Res 40(2):e14, 2012.

Li F, Ryu BY, Krueger RL, Heldt SA, Albritton LM. Targeted entry via somatostatin receptors using a novel modified retrovirus glycoprotein that delivers genes at levels comparable to those of wild-type viral glycoproteins. J Virol 86(1):373-81, 2012.

Bandaranayake AD, Correnti C, Ryu BY, Brault M, Strong RK, Rawlings DJ. Daedalus: a robust, turnkey platform for rapid production of decigram quantities of active recombinant proteins in human cell lines using novel lentiviral vectors. Nucleic Acids Res 39(21):e143, 2011.

Certo MT, Ryu BY, Annis JE, Garibov M, Jarjou J, Rawlings DJ, Scharenberg AM. Tracking genome engineering outcome at individual DNA breakpoints. Nat Methods 8(8):671-6, 2011.

Aubert M, Ryu BY, Banks L, Rawlings DJ, Scharenberg AM, Jerome KR. Successful targeting and disruption of an integrated reporter lentivirus using the engineered homing endonuclease Y2 I-AniI. PLoS One 6(2):e16825, 2011.

Sather BD, Ryu BY, Stirling BV, Garibov M, Kerns HM, Humblet-Baron S, Astrakhan A, Rawlings DJ. Development of B-lineage predominant lentiviral vectors for use in genetic therapies for B cell disorders. Mol Ther 19(3):515-25, 2011.

Kerns HM, Ryu BY, Stirling BV, Sather BD, Astrakhan A, Humblet-Baron S, Liggitt D, Rawlings DJ. B cell–specific lentiviral gene therapy leads to sustained B-cell functional recovery in a murine model of X-linked agammaglobulinemia. Blood 115:2146-55, 2010.

Ryu BY, Zavorotinskaya T, Trentin B, Albritton LM. The block to membrane fusion differs with the site of ligand insertion in modified retroviral envelope proteins. J Gen Virol 89:1049-58, 2008.

Ryu BY, Evans-Galea MV, Gray JT, Bodine DM, Persons DA, Nienhuis AW. An experimental system for the evaluation of retroviral vector design to diminish the risk for proto-oncogene activation. Blood 111(4):1866-75, 2008.

Ryu BY, Persons DA, Evans-Galea MV, Gray JT, Nienhuis AW. A chromatin insulator blocks interactions between globin regulatory elements and cellular promoters in erythroid cells. Blood Cells Mol Dis 39:221-228, 2007.

Seggewiss R, Pittaluga S, Adler RL, Guenaga FJ, Ferguson C, Pilz I, Ryu B, Sorrentino BP, Young WS, Donahue RE, von Kalle C, Nienhuis AW, Dunbar CE. Acute myeloid leukemia associated with retroviral gene transfer to hematopoietic progenitor cells in a rhesus macaque. Blood 107: 3865-67, 2006.

Ryu BY, Kwon TJ. Growth of Hansenula sp. MS-364 and formate dehydrogenase activity. Kor J Appl Microbiol Biotechnol 25:403-407, 1997. 

Last update: January 2018