Suzanne Jackowski, PhD
Suzanne Jackowski, PhD

Suzanne Jackowski, PhD

Member, St. Jude Faculty

Departments

Education

BA – Canisius College, Buffalo, New York
PhD – University of Tennessee, Graduate School of Biomedical Science, Oak Ridge National Laboratory

Research Interests

  • Regulation of phospholipid homeostasis
  • Control of intracellular coenzyme A content
  • Phospholipid metabolism and membrane stress responses
  • Pantothenate kinase as a target for small molecule therapeutics

Defining the links between membrane phospholipid synthesis and cell functions

The importance of Coenzyme A

Selected Publications

Corbin DR, Rehg JE, Shepard D, Stoilov P, Percifield RJ, Horner L, Frase S, Zhang Y-M, Rock CO, Hollander J, Jackowski S, Leonardi R.  Excess coenzyme A reduces skeletal muscle performance and strength in mice overexpressing human PANK2. Mol Genet Metab 120:350-362, 2017.

Subramanian C, Yun M-K, Yao J, Sharma LK, Lee RE, White SW, Jackowski S, Rock C.  Allosteric regulation of mammalian pantothenate kinase. J Biol Chem 291:22302-22314, 2016.

Jackowski S, Rock CO. CoA regulation and metabolic control. The Biochemist 37(1):4-8, 2015

Zano SP, Pate C, Frank M, Rock CO, Jackowski S. Correction of a genetic deficiency in pantothenate kinase 1 using phosphopantothenate replacement therapy. Mol Genet Metab 116:281-288, 2015.

Shumar SA, Pagone P, Alfonso-Pecchio A, Gray JT, Rehg JE, Jackowski S, Leonardi R. Induction of neuron-specific degradation of coenzyme A models pantothenate kinase-associated neurodegeneration by reduction motor coordination in mice. PLoS One 10:e0130013, 2015.

Selathurai A, Burch ML, Kowalski GM, Sepulveda P, Risis S, Lee-Young RS, Meikle PJ, Genders AJ, McGee SL, Watt MJ, Russell AP, Frank M, Jackowski S, Febbraio MA, Bruce CR.  Elimination of the CDP-ethanolamine pathway in skeletal muscle has pronounced effects on lipid homeostasis and mitochondrial oxidative capacity without altering insulin sensitivity. Cell Metab 21:718-730, 2015.

Sharma LK, Leonardi R, Lin W, Boyd VA, Goktug A, Shelat AA, Chen T, Jackowski S, Rock CO. A high-throughput screen reveals new small-molecule activators and inhibitors of pantothenate kinases. J Med Chem 58:1563-1568, 2015.

Jackowski S, Leonardi R. Deregulated coenzyme A, loss of metabolic flexibility and diabetes.  Biochem Soc Trans 42:1118-1122, 2014.

Dansie LE, Reeves S, Miller K, Zano SP, Frank M, Wang J, Jackowski S. Physiological roles of the pantothenate kinases. Biochem Soc Trans 42:1033-1036, 2014.

Leonardi R, Rock CO, Jackowski S. Pank1 deletion in leptin-deficient mice reduces hyperglycaemia and hyperinsulinaemia and modifies global metabolism without affecting insulin resistance. Diabetalogia 57:1466-1475, 2014.

Leonardi R, Jackowski S. Coenzyme A: when small is mighty. ASBMB Today 12(7):56-57, 2013.

Fagone P, Jackowski S. Phosphatidylcholine and the CDP-Choline Cycle. Biochimica Biophysica Acta 1831:523-32, 2013.

Brewer J, Jackowski S. UPR-mediated membrane biogenesis in B cells. Biochem Res Int [0nline] 2012:738471, 2012. 

Alfonso-Pecchio A, Garcia M, Leonardi R, Jackowski S. Compartmentalization of Mammalian Pantothenate Kinases. PLoS One 7:e49509, 2012. 

Garcia M, Leonardi R, Zhang Y-M, Rehg, JE, Jackowski S. Germline deletion of pantothenate kinases 1 and 2 reveals the key roles for CoA in postnatal metabolism. PLoS One 7:e40871, 2012.

Leonardi R, Subramanian C, Jackowski S, Rock CO. Cancer-associated isocitrate dehydrogenase mutations inactivate NADPH-dependent reductive carboxylation. J Biol Chem 287:14615-14620, 2012.

Aragon IV, Barrington R, Jackowski S, Mori K, Brewer JW. The specialized unfolded protein response of B lymphocytes: ATF6α-independent development of antibody-secreting B cells.  Mol Immunology 51:347-355, 2012.

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Siudeja K, Xu L, Srinivasan B, Rana A, de Jong J, Jackowski S, Hayflick S, Sibon OCM. Impaired coenzyme A metabolism affects histone and tubulin acetylation in Drosophila and human cell models of pantothenate kinase associated neurodegeneration. EMBO Mol Med 3:755-766, 2011.

Zhang K, Wang S, Malhotra J, Hassler JR, Back SH, Wang G, Chang L, Xu W, Leonardi R, Chen E, Jackowski S, Kaufman RJ. The unfolded protein response transducer IRE1α prevents ER stress-induced hepatic steatosis. EMBO J 30:1356-1375, 2011.

Rock CO, White SW, Jackowski S. Modulation of pantothenate kinase 3 activity by small molecules that interact with the substrate/allosteric regulatory domain. Chem Biol17(8):892-902, 2010.

Leonardi R, Rehg JE, Rock CO, Jackowski S. Pantothenate kinase 1 is required to support the metabolic transition from the fed to the fasted state. PLoS One 5(6):e11107, 2010.

Bommiasamy H, Back SH, Fagone P, Lee K, Meshinchi S, Vink E, Sriburi R, Frank M, Jackowski S, Kaufman RJ, Brewer JW. ATF6 and Induction of XBP-1-independent expansion of the endoplasmic reticulum. J Cell Science 122:1626-1636, 2009.

Fagone P, Gunter C, Sage CR, Gunn KE, Brewer JW, Jackowski S. CTP:phosphocholine cytidylyltransferase α is required for B-cell proliferation and class switch recombination. J Biol Chem 284:6847-6854, 2009.

Tian Y, Pate C, Andreolotti A, Wang L, Tuomanen E, Boyd K, Claro E, Jackowski S. Cytokine secretion requires phosphatidylcholine synthesis. J Cell Biol181:945-957, 2008.

Hong B-S, Senisterra G. Rabeh W, Vedadi M, Leonardi R, Zhang Y-M, Rock CO, Jackowski S, Park H-W. Crystal structures of human pantothenate kinases: Insight into allosteric regulation and neurodegeneration disorder. J Biol Chem 282:27984-27993, 2007.

Leonardi R, Zhang Y-M, Lykidis A, Rock CO, Jackowski S. Localization and regulation of mouse pantothenate kinase 2. FEBS Lett 581:4639-4644, 2007.

Gunter C, Frank M, Tian Y, Murti KG, Rehg JE, Jackowski S. Probucol therapy overcomes the reproductive defect in CTP:phosphocholine cytidylyltransferase ß2 Knockout Mice. Biochim Biophys Acta 1771: 845-852, 2007.

Zhang Y-M, Chohnan S, Virga KG, Stevens RD, Ilkayeva OR, Wenner BR, Bain JR, Newgard CB, Lee RE, Rock CO, Jackowski S. Chemical knockout of pantothenate kinase reveals the metabolic and genetic program responsible for hepatic coenzyme A homeostasis. Chem & Biol 14:291-302, 2007.

Fagone P, Sriburi R, Frank M, Wang J, Gunter C, Brewer JW, Jackowski S. Phospholipid biosynthesis program underlying membrane expansion during B-lymphocyte differentiation. J Biol Chem 282:7591-7605, 2007.

Sriburi R, Buldak GL, Robbins GR, Frank M, Jackowski S, Brewer JW. Coordinate regulation of phospholipid biosynthesis and secretory pathway gene expression in XBP-1(S)-induced endoplasmic reticulum biogenesis. J Biol Chem 282:7024-7034, 2007.

Leonardi R, Rock CO, Jackowski S, Zhang Y-M. Activation of human mitochondrial pantothenate kinase 2 by palmitoylcarnitine. Proc Nat Acad Sci USA104:1494-1499, 2007.

Nicely NI, Parsonage D, Paige C, Newton GL, Fahey RC, Leonardi R, Jackowski S, Mallett TC, Claiborne A. Structure of the type III pantothenate kinase from Bacillus anthracis at 2.0 A resolution: Implications for coenzyme A-dependent redox biology. Biochem46:3234-3245, 2007.

Tian Y, Zhou R, Rehg JE, Jackowski S. Role of phosphocholine cytidylyltransferase a in lung development. Mol Cell Biol 27:975-982, 2007.

Tian Y, Jackson P, Gunter C, Wang J, Rock CO, Jackowski S. Placental thrombosis and spontaneous fetal death in mice deficient in ethanolamine kinase 2. J Biol Chem 281:28438-28449, 2006.

Hong B-S, Yun M-K, Zhang Y-M, Chohnan S, Rock CO, White SW, Jackowski S, Park H-W, Leonardi R. Type II and type III pantothenate kinases: The same monomer fold creates dimers with distinct catalytic properties. Structure 14:1251-1261, 2006.

Zhang Y-M, Rock CO, Jackowski S. Biochemical properties of human pantothenate kinase 2 isoforms and mutations linked to pantothenate kinase-associated neurodegeneration. J Biol Chem 281:107-114, 2006.

Virga KG, Zhang Y-M, Leonardi R, Ivey RA, Hevener K, Park H-W, Jackowski S, Rock CO, Lee RE. Structure-activity relationships and enzyme inhibition of pantothenamide-type pantothenate kinase inhibitors. Bioorg Med Chem 14:1007-1020, 2006.

Zhang Y-M, Rock CO, Jackowski S. Feedback regulation of murine pantothenate kinase 3 by coenzyme A and coenzyme A thioesters. J Biol Chem280:32594-32601, 2005.

Wang L, Magdaleno S, Tabas I, Jackowski S. Early embryonic lethality in mice with targeted deletion of the CTP:phosphocholine cytidylyltransferase α (Pcyt1a) Gene. Mol Cell Biol 25:3357-3363, 2005.

Leonardi R, Chonan S, Zhang Y-M, Virga KG, Lee RE, Rock CO, Jackowski S. A pantothenate kinase from Staphylococcus aureus refractory to feedback regulation of coenzyme A. J Biol Chem 280:3314-3322, 2005.

Last update: June 2017