Janet F. Partridge, PhD
Janet F. Partridge PhD

Janet F. Partridge PhD

Associate Member, St. Jude Faculty



PhD – University of London, National Institute for Medical Research, London, United Kingdom

Research Interests

  • To understand how centromeric heterochromatin is formed
  • To identify new components of centromeric heterochromatin in both fission yeast and mammals through a combination of biochemical and genetic approaches
  • To determine whether mutations in mammalian factors contribute to aneuploidy and cancer progression

Selected Publications

Job G, Brugger C, Xu T, Lowe BR, Pfister Y, Qu C, Shanker S, Banos Sanz JI, Partridge JF, Schalch T. SHREC silences heterochromatin via distinct remodeling and deacetylation modules. Mol Cell 62(2):207-221, 2016.

Gal C, Murton HE, Subramanian L, Whale AJ, Moore KM, Paszkiewicz K, Codlin S, Bahler J, Creamer KM, Partridge JF, Allshire RC, Kent NA, Whitehall SK. Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome occupancy and organisation. EMBO Rep 17(1):79-93, 2016.

Kallappagoudar S, Yadav RK, Lowe BR, Partridge JF. Histone H3 mutations-a special role for H3.3 in tumorigenesis? Chromosoma 124(2):177-189, 2015.

Epling LB, Grace CR, Lowe BR, Partridge JF, Enemark EJ. Cancer-Associated Mutants of RNA Helicase DDX3X Are Defective in RNA-Stimulated ATP Hydrolysis. J Mol Biol 427(9):1779-96, 2015.

Creamer KM, Job G, Shanker S, Neale GA, Lin YC, Bartholomew B, Partridge JF. The Mi-2 homolog Mit1 actively positions nucleosomes within heterochromatin to suppress transcription. Mol Cell Biol 34(11):2046-61, 2014.

Huether R, Dong L, Chen X, Wu G, Parker M, Wei L, Ma J, Edmonson MN, Hedlund EK, Rusch MC, Shurtleff SA, Mulder HL, Boggs K, Vadordaria B, Cheng J, Yergeau D, Song G, Becksfort J, Lemmon G, Weber C, Cai Z, Dang J, Walsh M, Gedman AL, Faber Z, Easton J, Gruber T, Kriwacki RW, Partridge JF, Ding L, Wilson RK, Mardis ER, Mullighan CG, Gilbertson RJ, Baker SJ, Zambetti G, Ellison DW, Zhang J, Downing JR. The landscape of somatic mutations in epigenetic regulators across 1,000 paediatric cancer genomes. Nat Commun 5:3630, 2014.

Alper BJ, Job G, Yadav RK, Shanker S, Lowe BR, Partridge JF. Sir2 is required for Clr4 to initiate centromeric heterochromatin assembly in fission yeast. Embo J 32(17):2321-35, 2013.

Alper BJ, Lowe BR, Partridge JF. Centromeric heterochromatin assembly in fission yeast-balancing transcription, RNA interference and chromatin modification. Chromosome Res June 26, 2012.

Schalch T, Job G, Shanker S, Partridge JF*, Joshua-Tor L*. The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS. Nat Struct Mol Biol 18(12):1351-7, 2011. *Joint corresponding author.

Chen JS, Lu LX, Ohi MD, Creamer KM, English C, Partridge JF, Ohi R, Gould KL. Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. J Cell Biol 195(4):583-593, 2011.

Creamer KM, Partridge JF. RITS-connecting transcription, RNA interference, and heterochromatin assembly in fission yeast. Wiley Interdiscip Rev RNA 2(5):632-46, 2011.

Hansen KR, Hazan I, Shanker S, Watt S, Verhein-Hansen J, Bähler J, Martienssen RA, Partridge JF, Cohen A, Thon G. H3K9me-Independent Gene Silencing in Fission Yeast Heterochromatin by Clr5 and Histone Deacetylases. PLoS Genet 7(1):1-16, 2011.

Shanker S*, Job G*, George OL, Creamer KM, Shaban A, Partridge JF. Continuous requirement for the Clr4 complex but not RNAi for centromeric heterochromatin assembly in fission yeast harboring a disrupted RITS complex. PLOS Genetics 6(10):e1001174, 2010. *Joint first author.

Partridge JF. Spreading the silence. Developmental Cell 16: 630-632, 2009.

Schalch T, Job G, Noffsinger VJ, Shanker S, Kuscu C, Joshua-Tor L*, Partridge JF*. High affinity binding of Chp1 chromodomain to K9 methylated histone H3 is required to establish centromeric heterochromatin. Molecular Cell 34:36-46, 2009. *Joint corresponding author.

Partridge JF. Centromeric chromatin in fission yeast. Frontiers in Bioscience 13:3896-905, 2008.

DeBeauchamp JL*, Moses AA*, Noffsinger VJP, Ulrich D, Kosinski AM, Job G, Partridge JF. Chp1-Tas3 interaction is required to recruit RITS to fission yeast centromeres and for maintenance of centromeric heterochromatin. Mol Cell Biol 28:2154-2166, 2008. *Joint first author.

Partridge JF*, DeBeauchamp JL, Kosinski AM, Ulrich D, Hadler M, Noffsinger VJP. Functional separation of establishment and maintenance of centromeric heterochromatin. Molecular Cell 26:593-602, 2007. *Corresponding author.

Castillo AG*, Mellone B*, Partridge JF, Richardson W, Hamilton GL, Allshire RC, Pidoux AL. Plasticity of fission yeast CENP-A chromatin driven by relative levels of histone H3 and H4. PLoS Genet 3: e121, 1264-1274, 2007. *Joint first author.

Petrie VJ, Wuitschick JD, Givens CD, Kosinski AM, Partridge JF. RNA interference (RNAi)-dependent and RNAi-independent association of the Chp1 chromodomain protein with distinct heterochromatic loci in fission yeast. Mol Cell Biol 25:2331-2346, 2005.

Murchison EP, Partridge JF, Tam O, Cheloufi S, and Hannon GJ. Characterization of Dicer-Deficient Murine Embryonic Stem Cells. Proc Natl Acad Sci USA 102:12135-12140, 2005.

Mellone BG, Ball L, Suka N, Grunstein MR, Partridge JF, Allshire RC. Centromere function in fission yeast is governed by the amino terminus of histone H3. Curr Biol 13: 1748-1757, 2003.

Partridge JF, Scott KCS, Bannister, AJ, Kouzarides T, Allshire RC. A cis-acting element from fission yeast centromeres mediates histone H3 methylation and recruitment of silencing factors to an ectopic site. Curr Biol 12:1652-1660, 2002.

Schadick K, Fourcade HM, Boumenot P, Seitz JJ, Morrell JL, Chang L, Gould KL, Partridge JF, Allshire RC, Kitagawa K, Hieter P, Hoffman CS. The Schizosaccharomyces pombe git7 gene is required for glucose/cAMP signaling, cell wall integrity, and septation. Eukaryotic Cell 1:558-567, 2002.

Bernard P, Maure J-F, Partridge JF, Genier S, Javerzat JP, Allshire RC. Requirement of heterochromatin for cohesion at centromeres. Science 294:2539-2542, 2001.

Bannister AJ, Zegerman P, Partridge JF, Miska EA, Thomas JO, Allshire RC, Kouzarides T. Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain. Nature 410:120-124, 2001.

Partridge JF, Borgstrom B, Allshire RC. Distinct protein interaction domains and protein spreading in a complex centromere. Genes Dev 14:783-791, 2000.

Partridge JF, Allshire RC. Great chieftain o’ the fungal-race! A report of the 1st International Fission Yeast meeting, Edinburgh, 1999. Trends Genet 16:113-114, 2000

Cowieson NP, Partridge JF, Allshire RC, McLaughlin PJ. Dimerisation of a chromo shadow domain and distinctions from the chromo domain as revealed by structural analysis. Current Biology 10:517-525, 2000.

Ekwall K, Partridge JF. Fission yeast chromosome analysis: Fluorescent in situ hybridization (FISH) and chromatin immunoprecipitation (CHIP). In: Chromosome Structural Analysis: A Practical Approach (ed. W. Bickmore), Oxford University Press. 1999.

Olsson TGS, Ekwall K, Allshire RC, Sunnerhagen P, Partridge JF, Richardson WA. Genetic characterisation of hda1, a putative fission yeast histone deacetylase gene. Nucleic Acids Res 26:3247-3254, 1998.

McInerny CJ, Partridge JF, Mikesell GE, Creemer DP, Breeden LL. A novel Mcm1-dependent element in the SWI4, CLN3, CDC6 and CDC47 promoters activates M/G1-specific transcription. Genes Dev 11:1277-1288, 1997.

Partridge JF, Mikesell GE, Breeden LL. Cell cycle-dependent transcription of CLN1 involves Swi4 binding to MCB-like elements. J Biol Chem 272:9071-9077, 1997.

Girling R, Partridge JF, Bandara LR, Zamanian M, Totty N, Hsuan JJ, La Thangue NB. A new component of the transcription factor DRTF1/E2F. Nature 362:83-87, 1993.

Partridge JF, La Thangue NB. A developmentally regulated and tissue-dependent transcription factor complexes with the retinoblastoma gene product. EMBO J 10:3819-3827, 1991.

Last update: April 2016