Light Microscopy

The CTIC assists and trains St. Jude scientists in all aspects of imaging research including experimental design, execution of the experimental plan, image analysis, quantification, presentation and publication. The instrumentation in the CTIC supports a wide variety of imaging modalities, from the simplest to the most complex. Listed below are many of the techniques we routinely employ:

  • Confocal Microscopy, point-scanning or spinning disk (CLSM)
  • Fluorescence correlation spectroscopy (FCS)
  • Fluorescence recovery after photobleaching (FRAP)
  • Fluorescence resonance energy transfer (FRET)
  • Frequency domain fluorescence lifetime imaging (FLIM)
  • Ion imaging
  • Live cell imaging with environment control
  • Multiphoton imaging (MPE)
  • Sheetplane Illumination Microscopy (SPIM)
  • Single molecule imaging
  • Super resolution techniques including:
    • PALM (Photoactivated Light Microscopy)
    • STORM (Optical Reconstruction Microscopy)
    • SIM (Structured Illumination Microscopy)
  • Tiling of image montages
  • Time-lapse imaging
  • Total internal reflection fluorescence (TIRF)
  • Transmitting light imaging with contrast enhancement by Phase or DIC (Differential Interference contrast)
  • Widefield fluorescence and deconvolution