A Novel Method for Quantitative Detection of Purines and Their Metabolites (SJ-13-0014)

St. Jude Reference #SJ-13-0014

Description

Researchers have designed a new, relatively simple, direct and accurate method for the quantitative measurement of the purine neurotransmitter adenosine, other purines, and their metabolites. This method uses high pressure liquid chromatography coupled to an electrochemical detector (HPLC-ECD), equipment that is widely available.  This research could help in developing treatments for neurological disorders that would have fewer severe or long term side effects, and development of therapeutics for other areas that may be affected by adenosine.

Adenosine (ADO), a non-classical neurotransmitter and neuromodulator, and its metabolites adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP), have been shown to play an important role in a number of biochemical processes. Although their signaling is well described, it has been difficult to directly, accurately and simultaneously quantitate these purines in tissue or fluids. In their recent publication, they describe a novel method for measuring adenosine (ADO) and its metabolites using HPLC-ECD. Using this chromatographic technique, they examined baseline levels of ADO and ATP, ADP and AMP in 6 different brain regions of the C57BL/6J mouse: stratum, cortex, hippocampus, olfactory bulb, substantia nigra and cerebellum and compared ADO levels in 5 different strains of mice (C57BL/6J, Swiss-Webster, FVB/NJ, 129P/J, and BALB/c) to demonstrate that baseline levels of purines vary significantly among the brain regions as well as between different mouse strains. These dissimilarities in purine concentrations may explain the variable phenotypes among background strains described in neurological disease models.


 

Keywords

Measurement- Adenosine, purine, metabolite, neurotransmitter; high pressure liquid chromatography coupled to electrochemical detector (HPLC-ECD); neurological disease, Parkinson’s, Schizophrenia, Epilepsy, Stroke, Panic Disorder, immunological syndromes, cellular bioenergetics, transgenic mice, brain


 

Granted Patents or Published Applications

WO 2014/134379 A1 


 

Related Scientific References

Pani AK, Jiao Y, Sample KJ, Smeyne RJ (2014) Neurochemical Measurement of Adenosine in Discrete Brain Regions of Five Strains of Inbred Mice. PLoSONE 9(3): e92422. doi:10.1371/journal.pone.0092422


 

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