The Electron Microscopy core is a centralized facility operated by full-time, experienced staff and open to all St. Jude investigators. We offer specialized services and an array of instruments for scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

Services are strongly subsidized and include assistance with experimental design, sample preparation, image acquisition, image analysis, quantification, presentation, and publication. Experts are available to provide one-on-one consultations and training for investigators interested in learning new techniques.

We offer investigators the ability to harness electron microscopy approaches for a wide variety of biological questions. 

Techniques

  • Transmission electron microscopy 
  • Electron tomography (3D imaging)
  • Scanning electron microscopy
  • Focused ion beam scanning electron microscopy (3D imaging)
  • Serial block face scanning electron microscopy (3D imaging)
  • Immuno electron microscopy for scanning and transmission electron microscopy

Selected Publications

Fu G, Guy CS, Chapman NM, Palacios G, Wei J, Zhou P, Long L, Wang YD, Qian C, Dhungana Y, Huang H, Kc A, Shi H, Rankin S, Brown SA, Johnson A, Wakefield R, Robinson CG, Liu X, Sheyn A, Yu J, Jackowski S, Chi H. Metabolic control of TFH cells and humoral immunity by phosphatidylethanolamine. Nature Jul;595(7869):724-729, 2021.

Hall ET, Dillard ME, Stewart DP, Zhang Y, Wagner B, Levine RM, Pruett-Miller SM, Sykes A, Temirov J, Cheney RE, Mori M, Robinson CG, Ogden SK. Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complex. Elife Feb 11;10:e61432,2021.

Briard B, Fontaine T, Samir P, Place DE, Muszkieta L, Malireddi RKS, Karki R, Christgen S, Bomme P, Vogel P, Beau R, Mellado E, Ibrahim-Granet O, Henrissat B, Kalathur RC, Robinson C, Latgé JP, Kanneganti TD. Galactosaminogalactan activates the inflammasome to provide host protection. Nature Dec;588(7839):688-692, 2020.

Eom TY, Han SB, Kim J, Blundon JA, Wang YD, Yu J, Anderson K, Kaminski DB, Sakurada SM, Pruett-Miller SM, Horner L, Wagner B, Robinson CG, Eicholtz M, Rose DC, Zakharenko SS. Schizophrenia-related microdeletion causes defective ciliary motility and brain ventricle enlargement via microRNA-dependent mechanisms in mice. Nat Commun Feb 14;11(1):912, 2020.

Cortez V, Boyd DF, Crawford JC, Sharp B, Livingston B, Rowe HM, Davis A, Alsallaq R, Robinson CG, Vogel P, Rosch JW, Margolis E, Thomas PG, Schultz-Cherry S. Astrovirus infects actively secreting goblet cells and alters the gut mucus barrier. Nat Commun Apr 29;11(1):2097, 2020.

Norrie JL, Lupo MS, Xu B, Al Diri I, Valentine M, Putnam D, Griffiths L, Zhang J, Johnson D, Easton J, Shao Y, Honnell V, Frase S, Miller S, Stewart V, Zhou X, Chen X, Dyer MA. Nucleome Dynamics during Retinal Development. Neuron Nov 6;104(3):512-528.e11, 2019.

Aldiri I, Xu B, Wang L, Chen X, Hiler D, Griffiths L, Valentine M, Shirinifard A, Thiagarajan S, Sablauer A, Barabas ME, Zhang J, Johnson D, Frase S, Zhou X, Easton J, Zhang J, Mardis ER, Wilson RK, Downing JR, Dyer MA. The dynamic epigenetic landscape of the retina during development, reprogramming, and tumorigenesis. Neuron 94(3):550-568.e10, 2017.

Corbin DR, Rehg JE, Shepherd DL, Stoilov P, Percifield RJ, Horner L, Frase S, Zhang YM, Rock CO, Hollander JM, Jackowski S, Leonardi R. Excess coenzyme A reduces skeletal muscle performance and strength in mice overexpressing human PANK2. Mol Genet Metab 120(4):350-362, 2017.

Man SM, Karki R, Sasai M, Place DE, Kesavardhana S, Temirov J, Frase S, Zhu Q, Malireddi RK, Kuriakose T, Peters JL, Neale G, Brown SA, Yamamoto M, Kanneganti TD. IRGB10 liberates bacterial ligands for sensing by the AIM2 and caspase-11-NLRP3 inflammasomes. Cell 167(2):382-396.e17, 2016.

Fukuda Y, Cheong PL, Lynch J, Brighton C, Frase S, Kargas V, Rampersaud E, Wang Y, Sankaran VG, Yu B, Ney PA, Weiss MJ, Vogel P, Bond PJ, Ford RC, Trent RJ, Schuetz JD. The severity of hereditary porphyria is modulated by the porphyrin exporter and Lan antigen ABCB6. Nat Commun 7:12353, 2016.

Diouf B, Devaraju P, Janke LJ, Fan Y, Frase S, Eddins D, Peters JL, Kim J, Pei D, Cheng C, Zakharenko SS, Evans WE. Msh2 deficiency leads to dysmyelination of the corpus callosum, impaired locomotion, and altered sensory function in mice. Sci Rep 6:30757, 2016.

Campos Y, Qiu X, Gomero E, Wakefield R, Horner L, Brutkowski W, Han YG, Solecki D, Frase S, Bongiovanni A, d'Azzo A. Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier. Nat Commun 7:11876, 2016.

Liu Z, Man SM, Zhu Q, Vogel P, Frase S, Fukui Y, Kanneganti TD. DOCK2 confers immunity and intestinal colonization resistance to Citrobacter rodentium infection. Sci Rep 6:27814, 2016.

Quarato G, Guy CS, Grace CR, Llambi F, Nourse A, Rodriguez DA, Wakefield R, Frase S, Moldoveanu T, Green DR. Sequential Engagement of Distinct MLKL Phosphatidylinositol-Binding Sites Executes Necroptosis. Mol Cell 61:(4):589-601, 2016.

Milasta S, Dillon CP, Sturm OE, Verbist KC, Brewer TL, Quarato G, Brown SA, Frase S, Janke LJ, Perry SS, Thomas PG, Green DR. Apoptosis-inducing-factor-dependent mitochondrial function is required for T cell but not B cell function. Immunity 44:(1):88-102, 2016.

Morgan JA, Lynch J, Panetta JC, Wang Y, Frase S, Bao J, Zheng J, Opferman JT, Janke L, Green DM, Chemaitilly W, Schuetz JD. Apoptosome activation, an important molecular instigator in 6-mercaptopurine induced Leydig cell death. Sci Rep 5:16488, 2015.

Cheepala SB, Pitre A, Fukuda Y, Takenaka K, Zhang Y, Wang Y, Frase S, Pestina T, Gartner TK, Jackson C, Schuetz JD. The ABCC4 membrane transporter modulates platelet aggregation. Blood 126:(20):2307-2319, 2015.

Zhang Y, Li F, Wang Y, Pitre A, Fang ZZ, Frank MW, Calabrese C, Krausz KW, Neale G, Frase S, Vogel P, Rock CO, Gonzalez FJ, Schuetz JD. Maternal bile acid transporter deficiency promotes neonatal demise. Nat Commun 6:8186, 2015.

Yamashita T, Hakizimana P, Wu S, Hassan A, Jacob S, Temirov J, Fang J, Mellado-Lagarde M, Gursky R, Horner L, Leibiger B, Leijon S, Centonze VE, Berggren PO, Frase S. Auer M, Brownell WE, Fridberger A, Zuo J. Outer hair cell lateral wall structure constrains the mobility of plasma membrane proteins. PLoS Genet 11:(9):e1005500, 2015.

Hiler D, Chen X, Hazen J, Kupriyanov S, Carroll PA, Qu C, Xu B, Johnson D, Griffiths L, Frase S, Rodriguez AR, Martin G, Zhang J, Jeon J, Fan Y, Finkelstein D, Eisenman RN, Baldwin K, Dyer MA. Quantification of retinogenesis in 3D cultures reveals epigenetic memory and higher efficiency in iPSCs derived from rod photoreceptors. Cell Stem Cell 17:(1):101-115, 2015.