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The Protein Production Facility (PPF) exists to provide a large-scale protein expression and purification service to support the translation of discoveries in molecular and cellular biology to chemical and structural biology. The facility offers state-of-the-art technologies for protein expression/purification from either bacterial or insect cell systems. The facility is equipped with a 120 L fermentor for bacterial growth, several 10 L fermentors for bacterial and insect cell growth, AKTA Explorer purification systems and a robotic workstation for initial crystallization trials. The facility has worked with about 30 St. Jude investigators, expressing and purifying over 400 different proteins for use in structural and biochemical characterization, high-throughput screens, and use as reagents or antigens.