CTI Technologies

Electron Microscopy

JEOL 1200 EXII TEMSCAN analytical electron microscope. With a maximum kV of 125, the JEOL has the capabilities to do minimum electron dose imaging, and it has a side entry goniometer stage for stereo viewing. The JEOL has recently been enhanced with a new 11 megapixel AMT camera.

FEI Tecnai G² F20 TWIN Field Emission Gun Electron Microscope. The column of this 200 kV scope contains a factory aligned Schottky field emitter and isolated vacuum system for the field emission gun (FEG), diffraction lens, two condenser lenses, the TWIN objective lens with a computerized 5-axes eucentric goniometer, two projector lenses, an intermediate lens and a housing for the camera. The Tecnai G² has the capability to do automated tomography, low dose exposure, fast spot scan, and photomontage. This scope is equipped with an Eagle camera that is sensitive enough for cryo TEM.

Other technologies include a Leica AFS2 Freeze Substitution Unit, 4 Leica Ultramicrotomes (one with Cryo capabilities), a Leica DM 4000 with camera, a Leica IGL Immunostainer, 2 Lynx II automated Tissue Processors, a Leica TP automated Tissue Processor, and several image analysis computers.

Light Microscopy

Zeiss LSM 510 Meta: The Zeiss 510 is configured on an upright microscope with a variety of dry, oil immersion and water dipping lenses. The multiphoton capabilities of this instrument make it ideal for live cell imaging of thick biological specimens. Lasers include a multiline argon ion laser (457nm, 488nm and 514nm), 543nm and 647nm HeNe lasers, and a femtosecond pulsed laser, tunable from 580-1080nm. This system is configured with a precision motorized stage, enabling multi-point time-lapse experiments as well as high-resolution large specimen montage creation. The Zeiss 510 Meta also has multispectral capabilities, allowing the emission signals from closely overlapping fluorophore to be resolved.

Two Intelligent Imaging Innovations Marianas Systems: The Marianas systems are multimodal imaging workstations featuring CSU-X spinning disk confocal heads. Each is configured on a Zeiss AxioObserver microscope with 6 diode lasers providing the following wavelengths: 405nm, 442nm, 488nm, 515nm, 561nm, and 638nm. Designed primarily for live cell imaging experiments, these systems are equipped with full incubator enclosures, affording excellent cell viability during long term time-lapse experiments. The spinning disk confocal approach is advantageous in that it offers decreased photobleaching and phototoxicity as well as increased sensitivity and speed over standard point scanning confocal methods. One of the systems is equipped with a femtosecond pulsed laser and a Vector scanner for multiphoton imaging and photomanipulation techniques. These systems support a variety of imaging modalities and applications, including FLIM, FRET, FRAP, and FCS, for the study of protein-protein interactions and molecular dynamics. TIRF, which provides a thin optical section at the specimen-coverslip interface, offers a large S/N advantage for studying events at membrane surfaces such as vesicle trafficking. Additional techniques include widefield microscopy, deconvolution and calcium imaging.

Nikon C1Si: The C1Si is a spectral laser scanning confocal microscope with the following 4 diode lasers: 404nm, 488nm, 561nm, and 638nm. Configured on a Nikon TE2000 inverted microscope and fitted with an incubator enclosure, this system is utilized for both live and fixed samples. The spectral detector, which allows fluorescence emission spectra to be obtained for an image on a pixel-by-pixel basis, is useful for resolving closely overlapping fluorophores, separating specific signal from background autofluorescence, and performing spectral FRET. Another unique feature of this system is the integrated Perfect Focus System (PFS), a dynamic focus correction system that tracks the position of the coverslip with infrared light and corrects for any drift in focus with millisecond temporal resolution.

Additional resources and instrumentation include a Nikon TE2000 with an Eppendorf Microinjection system; a Leica SP1 laser scanning confocal with 488nm, 561nm and 638nm lasers; a Nikon E800 microscope equipped with color and monochrome cameras for brightfield, darkfield and fluorescence imaging and two 64-bit workstations with two Intel Xeon X5450 3.0 GHz Quad Core Processors, 16 GB of RAM, and a variety of software packages for image analysis including NIS Elements, Imaris, SlideBook, ImagePro Plus, Igor Pro, and MATLAB.