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Shondra Pruett-Miller, PhD
Shondra Pruett-Miller, PhD

Shondra Pruett-Miller, PhD

Assistant Member, St. Jude Faculty

  • Director, Center for Advanced Genome Engineering
  • Associate Director, Shared Resources, Comprehensive Cancer Center

Departments

Education

PhD - University of Texas Southwestern Medical School, Dallas, Texas
BS
- University of Arkansas, Fayetteville, Arkansas

Research Interests

  • Genome-editing technologies
  • Disease modeling in iPSCs
  • Development of preclinical animal models using CRISPR-Cas9
  • Development of novel genome editing strategies to treat β-hemoglobinopathies

Selected Publications

Wagner J, Wickman E, Shaw TI, Allo Anido A, Langfitt D, Zhang J, Porter SN, Pruett-Miller SM, Tillman H, Krenciute G, Gottschalk S Antitumor effects of CAR T cells redirected to the EDB splice variant of fibronectin. Cancer Immunol Res Dec 22:canimm.0280.2020, 2020. doi: 10.1158/2326-6066.CIR-20-0280. Online ahead of print. PMID: 3335518832.

Cheng X, Peters ST, Pruett-Miller SM, Saunders TL, Joe B. In Vivo CRISPR/Cas9-Based Targeted Disruption and Knockin of a Long Noncoding RNA. Methods Mol Biol 2021;2254:305-321, 2021. doi: 10.1007/978-1-0716-1158-6_19. PMID: 33326084

Smith KH, Budhraja A, Lynch J, Roberts K, Panetta JC, Connelly JP, Turnis ME, Pruett-Miller SM, Schuetz JD, Mullighan CG, Opferman JT. The Heme-regulated Inhibitor Pathway Modulates Susceptibility of Poor Prognosis B-Lineage Acute Leukemia to BH3-Mimetics. Mol Cancer Res Dec 7:molcanres.0586.2020, 2020. doi: 10.1158/1541-7786.MCR-20-0586. Online ahead of print. PMID: 33288732

**Pruett-Miller SM. Assessing Off-Target Editing of CRISPR-Cas9 Systems. CRISPR J  Dec;3(6):430-432, 2020. doi: 10.1089/crispr.2020.29116.smi. PMID: 33346715

Xu P, Scott DC, Xu B, Yao Y, Feng R, Cheng L, Mayberry K, Bi W, Palmer LE, King MT, Wang H, Li Y, Fan Y, Alpi AF, Li C, Peng J, Papizan J, Pruett-Miller SM, Spallek R, Bassermann F, Cheng Y, Schulman BA, Weiss MJ. FBXO11-Mediated Proteolysis of BAHD1 Relieves PRC2-dependent Transcriptional Repression in Erythropoiesis. Blood. Nov 6:blood.2020007809, 2020. doi: 10.1182/blood.2020007809. Online ahead of print. PMID: 33156908

Craig JE, Miller JN, Rayavarapu RR, Hong Z, Bulut GB, Zhuang W, Sakurada SM, Temirov J, Low JA, Chen T, Pruett-Miller SM, Huang LJ, Potts MB. MEKK3-MEK5-ERK5 signaling promotes mitochondrial degradation. Cell Death Discov Oct 20;6:107, 2020. doi: 10.1038/s41420-020-00342-7. eCollection 2020. PMID: 33101709

McGowan J, Peter C, Kim J, Popli S, Veerman B, Saul-McBeth J, Conti H, Pruett-Miller SM, Chattopadhyay S, Chakravarti R.14-3-3ζ-TRAF5 axis governs interleukin-17A signaling. Proc Natl Acad Sci U S A Oct 6;117(40):25008-25017, 2020. doi: 10.1073/pnas.2008214117. Epub Sep 23, 2020. PMID: 32968020

Demirci S, Zeng J, Wu Y, Uchida N, Shen AH, Pellin D, Gamer J, Yapundich M, Drysdale C, Bonanno J, Bonifacino AC, Krouse A, Linde NS, Engels T, Donahue RE, Haro-Mora JJ, Leonard A, Nassehi T, Luk K, Porter SN, Lazzarotto CR, Tsai SQ, Weiss M, Pruett-Miller SM, Wolfe SA, Bauer DE, Tisdale JF.BCL11A enhancer edited hematopoietic stem cells persist in rhesus monkeys without toxicity. J Clin Invest Sep 8:140189, 2020. doi: 10.1172/JCI140189. Online ahead of print. PMID: 32897878

Lee AK, Klein J, Fon Tacer K, Lord T, Oatley MJ, Oatley JM, Porter SN, Pruett-Miller SM, Tikhonova EB, Karamyshev AL, Wang YD, Yang P, Korff A, Kim HJ, Taylor JP, Potts PR. Translational Repression of G3BP in Cancer and Germ Cells Suppresses Stress Granules and Enhances Stress Tolerance. Mol Cell Aug 20;79(4):645-659.e9, 2020. doi: 10.1016/j.molcel.2020.06.037. Epub Jul 20, 2020. PMID: 32692974

Pinto EM, Figueiredo BC, Chen W, Galvao HCR, Formiga MN, Fragoso MCBV, Ashton-Prolla P, Ribeiro EMSF, Felix G, Costa TEB, Savage SA, Yeager M, Palmero EI, Volc S, Salvador H, Fuster-Soler JL, Lavarino C, Chantada G, Vaur D, Odone-Filho V, Brugières L, Else T, Stoffel EM, Maxwell KN, Achatz MI, Kowalski L, de Andrade KC, Pappo A, Letouze E, Latronico AC, Mendonca BB, Almeida MQ, Brondani VB, Bittar CM, Soares EWS, Mathias C, Ramos CRN, Machado M, Zhou W, Jones K, Vogt A, Klincha PP, Santiago KM, Komechen H, Paraizo MM, Parise IZS, Hamilton KV, Wang J, Rampersaud E, Clay MR, Murphy AJ, Lalli E, Nichols KE, Ribeiro RC, Rodriguez-Galindo C, Korbonits M, Zhang J, Thomas MG, Connelly JP, Pruett-Miller S, Diekmann Y, Neale G, Wu G, Zambetti.  GPXAF1 as a modifier of p53 function and cancer susceptibility. Sci Adv Jun 24;6(26):eaba3231, 2020. doi: 10.1126/sciadv.aba3231. eCollection Jun 2020. PMID: 32637605

Nguyen P, Okeke E, Clay M, Haydar D, Justice J, O'Reilly C, Pruett-Miller S, Papizan J, Moore J, Zhou S, Throm R, Krenciute G, Gottschalk S, DeRenzo C.Route of 41BB/41BBL Costimulation Determines Effector Function of B7-H3-CAR.CD28ζ T Cells. Mol Ther Oncolytics Jun 23;18:202-214, 2020. doi: 10.1016/j.omto.2020.06.018. eCollection Sep 25, 2020. PMID: 32728609

Kesavardhana S, Malireddi RKS, Burton AR, Porter SN, Vogel P, Pruett-Miller SM, Kanneganti TD. The Zα2 domain of ZBP1 is a molecular switch regulating influenza-induced PANoptosis and perinatal lethality during development. J Biol Chem Jun 12;295(24):8325-8330, 2020. doi: 10.1074/jbc.RA120.013752. Epub Apr 29, 2020. PMID: 32350114

Mondal B, Jin H, Kallappagoudar S, Sedkov Y, Martinez T, Sentmanat MF, Poet GJ, Li C, Fan Y, Pruett-Miller SM, Herz HM.The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. Elife Apr 16;9:e57519, 2020. doi: 10.7554/eLife.57519. PMID: 32297854

Zeineldin M, Federico S, Chen X, Fan Y, Xu B, Stewart E, Zhou X, Jeon J, Griffiths L, Nguyen R, Norrie J, Easton J, Mulder H, Yergeau D, Liu Y, Wu J, Van Ryn C, Naranjo A, Hogarty MD, Kamiński MM, Valentine M, Pruett-Miller SM, Pappo A, Zhang J, Clay MR, Bahrami A, Vogel P, Lee S, Shelat A, Sarthy JF, Meers MP, George RE, Mardis ER, Wilson RK, Henikoff S, Downing JR, Dyer MA. MYCN amplification and ATRX mutations are incompatible in neuroblastoma. Nat Commun Feb 14;11(1):913, 2020. doi: 10.1038/s41467-020-14682-6. PMID: 32060267

**Pruett-Miller SM. Cancer Screens: Better Together. CRISPR J Feb;3(1):12-14, 2020. doi: 10.1089/crispr.2020.29084.spm. PMID: 32091251

Eom TY, Han SB, Kim J, Blundon JA, Wang YD, Yu J, Anderson K, Kaminski DB, Sakurada SM, Pruett-Miller SM, Horner L, Wagner B, Robinson CG, Eicholtz M, Rose DC, Zakharenko SS.Schizophrenia-related microdeletion causes defective ciliary motility and brain ventricle enlargement via microRNA-dependent mechanisms in mice. Nat Commun Feb 14;11(1):912, 2020. doi:10.1038/s41467-020-14628-y.PMID: 32060266

Yang SW, Li L, Connelly JP, Porter SN, Kodali K, Gan H, Park JM, Tacer KF, Tillman H, Peng J, Pruett-Miller SM, Li W, Potts PR. A Cancer-Specific Ubiquitin Ligase Drives mRNA Alternative Polyadenylation by Ubiquitinating the mRNA 3' End Processing Complex. Mol Cell Mar 19;77(6):1206-1221.e7, 2020. doi: 10.1016/j.molcel.2019.12.022. Epub Jan 21, 2020. PMID: 31980388

Métais JY, Doerfler PA, Mayuranathan T, Bauer DE, Fowler SC, Hsieh MM, Katta V, Keriwala S, Lazzarotto CR, Luk K, Neel MD, Perry SS, Peters ST, Porter SN, Ryu BY, Sharma A, Shea D, Tisdale JF, Uchida N, Wolfe SA, Woodard KJ, Wu Y, Yao Y, Zeng J, Pruett-Miller S, Tsai SQ, Weiss MJGenome editing of HBG1 and HBG2 to induce fetal hemoglobin. Blood Adv Nov 12;3(21):3379-3392, 2019. doi: 10.1182/bloodadvances.2019000820.  PMID 31698466.

Fon Tacer K, Montoya MC, Oatley MJ, Lord T, Oatley JM, Klein J, Ravichandran R, Tillman H, Kim M, Connelly JP, Pruett-Miller SM, Bookout AL, Binshtock E, Kamiński MM, Potts PR. MAGE cancer-testis antigens protect the mammalian germline under environmental stress. Sci Adv May 29;5(5):eaav4832, 2019. doi: 10.1126/sciadv.aav4832. eCollection May, 2019. PMID: 31149633

**Connelly JP, Pruett-Miller SM. CRIS.py: A Versatile and High-throughput Analysis Program for CRISPR-based Genome Editing. Sci Rep Mar 12;9(1):4194, 2019. doi: 10.1038/s41598-019-40896-w. PMID:30862905

Wang B, Maxwell BA, Joo JH, Gwon Y, Messing J, Mishra A, Shaw TI, Ward AL, Quan H, Sakurada SM, Pruett-Miller SM, Bertorini T, Vogel P, Kim HJ, Peng J, Taylor JP, Kundu M. ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97. Mol Cell May 16;74(4):742-757.e8, 2019. doi: 10.1016/j.molcel.2019.03.027. Epub Apr 9, 2019.  PMID: 30979586

Waghulde H, Cheng X, Galla S, Mell B, Cai J, Pruett-Miller SM, Vazquez G, Patterson A, Vijay Kumar M, Joe B. Attenuation of Microbiotal Dysbiosis and Hypertension in a CRISPR/Cas9 Gene Ablation Rat Model of GPER1. Hypertension Nov;72(5):1125-1132, 2018. doi: 10.1161/HYPERTENSIONAHA.118.11175. PMID: 30354811

**Chen YH, Pruett-Miller SM. Improving single-cell cloning workflow for gene editing in human pluripotent stem cells.  Stem Cell Res Aug;31:186-192, 2018. doi: 10.1016/j.scr.2018.08.003. Epub 2018 Aug 4. PMID:30099335

Wang B, Joo JH, Mount R, Teubner BJW, Krenzer A, Ward AL, Ichhaporia VP, Adams EJ, Khoriaty R, Peters ST, Pruett-Miller SM, Zakharenko SS, Ginsburg D, Kundu M. The COPII cargo adapter SEC24C is essential for neuronal homeostasis. J Clin Invest Aug 1;128(8):3319-3332, 2018. doi: 10.1172/JCI98194. Epub 2018 Jun 25. PMID: 29939162

Churchman ML, Qian M, Te Kronnie G, Zhang R, Yang W, Zhang H, Lana T, Tedrick P, Baskin R, Verbist K, Peters JL, Devidas M, Larsen E, Moore IM, Gu Z, Qu C, Yoshihara H, Porter SN, Pruett-Miller SM, Wu G, Raetz E, Martin PL, Bowman WP, Winick N, Mardis E, Fulton R, Stanulla M, Evans WE, Relling MV, Pui CH, Hunger SP, Loh ML, Handgretinger R, Nichols KE, Yang JJ, Mullighan CG.Germline Genetic IKZF1 Variation and Predisposition to Childhood Acute Lymphoblastic Leukemia. Cancer Cell May 14;33(5):937-948.e8, 2018. doi: 10.1016/j.ccell.2018.03.021. Epub 2018 Apr 19.PMID: 29681510

**Pruett-Miller SM. It's CRISPR Clear: Off-Target Study Misses the Mark. CRISPR J Apr;1:130-131, 2018. doi: 10.1089/crispr.2018.29013.mil. PMID:31021203

Show More

Stewart DP, Marada S, Bodeen WJ, Truong A, Sakurada SM, Pandit T, Pruett-Miller SM, Ogden SK.Cleavage activates dispatched for Sonic Hedgehog ligand release. Elife Jan 23;7, 2018. pii: e31678. doi: 10.7554/eLife.31678. PMID: 29359685

**Sentmanat MF, Peters ST, Florian CP, Connelly JP, Pruett-Miller SM. A Survey of Validation Strategies for CRISPR-Cas9 Editing. Sci Rep Jan 17;8(1):888, 2018. doi: 10.1038/s41598-018-19441-8. PMID: 29343825

Cheng X, Waghulde H, Mell B, Morgan EE, Pruett-Miller SM, Joe B. Positional cloning of quantitative trait nucleotides for blood pressure and cardiac QT-interval by targeted CRISPR/Cas9 editing of a novel long non-coding RNA. PLoS Genet Aug 21;13(8):e1006961, 2017. doi: 10.1371/journal.pgen.1006961. eCollection 2017 Aug. PMID: 28827789

Wen Y, Liao G, Pritchard T, Zhao TT, Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB.  A Stable but Reversible Integrated Surrogate Reporter for Assaying CRISPR/Cas9-Stimulated Homology-directed Repair. J Biol Chem Feb 22, 2017. pii: jbc.M117.777722. doi: 10.1074/jbc.M117.777722. Epub 2017 Feb 22. PMID: 28228480

Cohen AJ, Saiakhova A, Corradin O, Luppino JM, Lovrenert K, Bartels CF, Morrow JJ, Mack SC, Dhillon G, Beard L, Myeroff L, Kalady MF, Willis J, Bradner JE, Keri RA, Berger NA, Pruett-Miller SM, Markowitz SD, Scacheri PC. Hotspots of aberrant enhancer activity punctuate the colorectal cancer epigenome. Nat Commun Feb 7;8:14400, 2017. doi: 10.1038/ncomms14400. PMID: 28169291

Orchard RC, Wilen CB, Doench JG, Baldridge MT, McCune BT, Lee YC, Lee S, Pruett-Miller SM, Nelson CA, Fremont DH, Virgin HW. Discovery of a proteinaceous cellular receptor for a norovirus. Science Aug 26; 353(6302):933-6, 2016. doi: 10.1126/science.aaf1220. Epub 2016 Aug 18. PubMed PMID: 27540007

Redmann V, Lamb CA, Hwang S, Orchard RC, Kim S, Razi M, Milam A, Park S, Yokoyama CC, Kambal A, Kreamalmeyer D, Bosch MK, Xiao M, Green K, Kim J, Pruett-Miller SM, Ornitz DM, Allen PM, Beatty WL, Schmidt RE, DiAntonio A, Tooze SA, Virgin HW. Clec16a is Critical for Autolysosome Function and Purkinje Cell Survival. Sci Rep 6:23326, 2016. PMID: 26987296

*Pruett-Miller SM, Davis GD. Donor plasmid design for codon and single base genome editing using zinc finger nucleases. Method Mol Bio 1239:219-29, 2015. PMID: 25408411

Chen F, Pruett-Miller SM, Davis GD. Gene editing using ssODNs with engineered endonucleases. Methods Mol Bio 1239:251-65, 2015. PMID: 25408408

Duda K, Lonowski LA, Kofoed-Nielsen M, Ibarra A, Delay CM, Kang Q, Yang Z, Pruett-Miller SM, Bennett EP, Wandall HH, Davis GD, Hansen SH, Frodin M. High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases of CRISPR/Cas9 nickase pairs. Nucleic Acids Research Apr 21, 2014. PMID: 24753413

Voit RA, Hendel A, Pruett-Miller SM, Porteus MH. Nuclease-mediated gene editing by homologous recombination of the human globin locus. Nucleic Acids Research Jan;42(2):1365-78, 2014. PMID: 24157834

Wilson KA, McEwen AE, Pruett-Miller SM, Zhang J, Kildebeck EJ, Porteus MH. Expanding the Repertoire of target sites for zinc finger nuclease-mediated genome modification. Molecular Therapy Nucleic Acids 2:e88, 2013. PMID: 23632390

Chen F, Pruett-Miller SM, Huang Y, Gjoka M, Duda K, Taunton J, Frodin M, Davis GD. High Frequency Genome Editing Using ssDNA with Zinc Finger Nucleases. Nature Methods 753-755, 2011. PMID: 21765410

Davis GD, Pruett-Miller SM. ZFN Donor Design; Codon and Single Base Genome Editing Using Zinc Finger Nucleases. Biowire 39-43, 2010.

Connelly JP, Barker JC, Pruett-Miller S, Porteus MH. Gene Correction by Homologous Recombination with Zinc Finger Nucleases in Primary Cells from a Mouse Model of a Generic Recessive Genetic Disease. Molecular Therapy 1103-10, 2010. PMID: 20389291

Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, Cheng L. Gene Targeting of a Disease-related Gene in Human Induced Pluripotent Stem and Embryonic Cells. Cell Stem Cell 97-110, 2009. PMID: 19540188

*Pruett-Miller SM, Reading D, Porter SN, Porteus MH. Attenuation of Zinc Finger Nuclease Cytotoxicity by Regulating Protein Expression. PLoS Genetics e1000376, 2009. PMID: 19214211

Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, Eichtinger M, Jiang T, Foley JE, Winfrey RF, Townsend JA, Unger-Wallace E, Sander JD, Muller-Lerch Felix, Pearlberg J, Gobel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK. Efficient Gene Modification Enabled by an OPEN (Oligomerized Pool ENgineering) Platform for Constructing Zinc Finger Nucleases. Molecular Cell 294-301, 2008. PMID: 18657511

*Pruett-Miller SM, Connelly JP, Maeder ML, Joung JK, Porteus MH. Optimizing Zinc Finger Nucleases for Gene Targeting in Mammalian Cells. Molecular Therapy 707-717, 2008. PMID: 18334988

Porteus MH, Connelly JP, Pruett SM. A Look to the Future Directions in Gene Therapy Research for Monogenic Diseases. PLOS Genetics 1285-1292, 2006. PMID: 17009872

Last update: February 2021