Stephen White, DPhil
Stephen White, DPhil

Stephen White, DPhil

Member, St. Jude Faculty

  • President and Dean, St. Jude Graduate School of Biomedical Sciences
  • Adjunct Full Professor, University of Tennessee Health Science Center



BS – University of Bristol, United Kingdom (1974)
DPhil – University of Oxford, United Kingdom (1978)

Research Interests

  • Structure and function of biological macromolecules and structure-based drug design
  • Enzyme mechanisms
  • DNA repair, recombination and DNA helicases

Bacterial Fatty Acid Synthesis        

Protein-DNA Interactions     

Structural Studies of the Prokaryotic Ribosome         

Selected Publications

He X, Byrd AK, Yun MK, Pemble CW 4th, Harrison D, Yeruva L, Dahl C, Kreuzer KN, Raney KD, White SW. The T4 phage SF1B helicase Dda is structurally optimized to perform DNA strand separation. Structure May 30, 2012.

Baughman BM, Slavish PJ, DuBois RM, Boyd VA, White SW, Webb TR. Identification of Influenza Endonuclease Inhibitors in a Targeted Fragment Library via a Novel Fluorescence Polarization Assay. ACS Chemical Biology 2012 (in press).

Yun M-K, Wu Y, Li Z, Zhao Y, Waddell MB, Ferreira AM, Lee RE, Bashford D, White SW. Catalysis and sulfa drug resistance in dihydropteroate synthase. Science 335:1110-14, 2012.

Gajewski S, Comeaux EQ, Jafari N, Bharatham N, Bashford D, White SW, van Waardenburg RCAM. Analysis of the active-site mechanism of Tyrosyl-DNA phosphodiesterase: A member of the phospholipase D superfamily. J Mol Biol 415:741-58, 2012.

Zhao Y, Hammoudeh D, Lin W, Das S, Yun MK, Li Z, Griffith E, Chen T, White SW, Lee RE. Development of a pterin-based fluorescent probe for screening dihydropteroate synthase. Bioconjug Chem 22:2110-7, 2011.

DuBois RM, Zaraket H, Reddivari M, Heath RJ, White SW, Russell CJ. Acid stability of the hemagglutinin protein regulates H5N1 influenza virus pathogenicity. PloS Pathog 7:e1002398, 2011.

Qi J, Virga KG, Das S, Zhao Y, Yun MK, White SW, Lee RE. Synthesis of bi-substrate state mimics of dihydropteroate synthase as potential inhibitors and molecular probes. Bioorg Med Chem 19:1298-1305, 2011.

Dubois RM, Aguilar-Yanez JM, Mendoza-Ochoa GI, Oropeza-Almazan Y, Schultz-Cherry S, Alvarez MM, White SW, Russell CJ. The receptor-binding domain of influenza virus hemagglutinin produced in Escherichia coli folds into its native, immunogenic structure. J Virol 85:855-872, 2011.

Gajewski S, Webb MR, Galkin V, Egelman EH, Kreuzer KN, White SW. Crystal structure of the phage T4 recombinase UvsX and its functional interaction with the T4 SF2 helicase UvsW. J Mol Biol405:65-76, 2011.

Pemble CW IV, Mehta PK, Mehra S, Li Z, Nourse A, Lee RE, White SW. Crystal structure of the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase•dihydropteroate synthase bifunctional enzyme from Francisella tularensis. PLoS One 5:e14165, 2010.

Leonardi R, Zhang YM, Yun MK, Zhou R, Zeng FY, Lin W, Cui J, Chen T, Rock CO, White SW, Jackowski S. Modulation of pantothenate kinase 3 activity by small molecules that interact with the substrate/allosteric regulatory domain. Chem Biol 17:892-902, 2010.

Aguilar-Yanez JM, Portillo-Lara R, Mendoza-Ochoa GI, Garcia-Echauri SA, Lopez-Pacheco F, Bulnex-Abundis D, Salgado-Gallegos J, Lara-Mayorga IM, Webb-Vargas Y, Leon-Angel FO, Rivero-Aranda RE, Oropeza-Almazan Y, Ruiz-Palacios GM, Zertuche-Guerra MI, Dubois RM, White SW, Schulz-Cherry S, Russell CJ, Alvarez MM. An influenza A/H1N1/2009 hemagglutinin vaccine produced in E. coli. PLos One 5:e11694, 2010.

Miller DJ, Zhang YM, Subramanian C, Rock CO, White SW. Structural basis for the transcription regulation of membrane lipid homeostasis. Nat Struct Mol Biol 17:971-975, 2010.

Hevener KE, Yun M-K, Qi J, Kerr ID, Babaoglu K, Hurdle JG, Balakrishnan K, White SW, Lee RE. Structural studies of pterin-based inhibitors of dihydropteroate synthase. J Med Chem 53:166-177, 2009.

Zhao W, Hevener KE, White SW, Lee RE, Boyett JM. A statistical framework to evaluate virtual screening. BMC Bioinformatics 10:225, 2009.

Hevener KE, Zhao W, Ball DM, Babaoglu K, Qi J, White SW, Lee RE. Validation of molecular docking programs for virtual screening against dihydropteroate synthase. J Chem Inf Model 49:444-460, 2009.

Jerga A, Miller DJ, White SW, Rock CO. Interfacial binding and conformational change in a soluble diacylglycerol kinase. J Biol Chem 284:7246-7254, 2009.

Miller DJ, Jerga A, Rock CO, White SW. Analysis of the Staphylococcus aureus DgkB structure reveals a common catalytic mechanism for the soluble diacylglycerol kinases. Structure 16:1036-1046, 2008.

Kerr ID, Sivakolundu S, Li Z, Buchsbaum JC, Knox LA, Kriwacki RW, White SW. Crystallographic and NMR analyses of UvsW and UvsW.1 from bacteriophage T4. J Biol Chem 282:34392-34400, 2007.

He X, van Waardenburg RC, Babaoglu K, Price AC, Nitiss KC, Nitiss JL, Bjornsti MA, White SW. Mutation of a conserved active site residue converts tyrosyl-DNA phosphodiesterase I into a DNA topoisomerase I-dependent poison. J Mol Biol 372:1070-1081, 2007.

Yun M, Nourse A, White SW, Rock CO, Heath RJ. Crystal structure and allosteric regulation of the cytoplasmic Escherichia coli L-asparaginase 1. J Mol Biol 369:794-811, 2007.

Hong BS, Yun MK, Zhang Y-M, Chohnan S, Rock CO, White SW, Jackowski S, Park H-W, Leonardi R. Prokaryotic type II and type III pantothenate kinases: The same monomer fold creates dimers with distinct catalytic properties. Structure 14:1251-1261, 2006.

Zeitoun O, Santos NMD, Gardner LA, White SW, Bahouth SW. Mutagenesis within helix 6 of the human {beta}1-adrenergic receptor identifies lysine324 as a residue involved in imparting the high-affinity binding state of agonists. Molecular Pharmacology 70:838-850, 2006.

Nitiss KC, Malik M, He X, White SW, Nitiss JL. Tyrosyl DNA phosphodiesterase 1 (Tdp1) participates in the repair of Top2 mediated DNA damage. Proc Natl Acad Sci 103:8953-8958, 2006.

Santos NMD, Gardner LA, White SW, Bahouth SW. Characterization of the residues in helix 8 of the human beta 1-adrenergic receptor that are involved in coupling the receptor to G proteins. J Biol Chem 281:12896-12907, 2006.

Zhang Y-M, White SW, Rock CO. Inhibiting bacterial fatty acid synthesis. J Biol Chem 281:17541-17544, 2006.

Miller DJ, Zhang Y-M, Rock CO, White SW. Structure of RhIG, an essential beta-ketoacyl reductase in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa. J Biol Chem 281:18025-18032, 2006.

Zhang Y-M, Hurlbert J, White SW, Rock CO. Roles of the active site water, histidine-303 and phenylalanine-396 in the catalytic mechanism of the elongation condensing enzyme of streptococcus pneumoniae. J Biol Chem 281:17390-17399, 2006.

Kim P, Zhang YM, Shenoy G, Nguyen QA, Boshoff HI, Manjunatha UH, Goodwin MB, Lonsdale J, Price AC, Miller DJ, Duncan K, White SW, Rock CO, Barry CE III, Dowd CS. Structure-activity relationships at the 5-position of thiolactomycin: an intact (5R)-isoprene unit is required for activity against the condensing enzymes from Mycobacterium tuberculosis and Escherichia coli. J Med Chem 49:159-171, 2006.

Last update: June 2012