Jie Zheng, PhD

Jie Zheng, PhD

Associate Member, St. Jude Faculty
Adjunct Associate Professor, Department of Molecular Sciences, University of Tennessee Health Science Center


Structural Biology

Contact Information

Jie Zheng, PhD
Structural Biology
MS 311, Room D1024B
St. Jude Children's Research Hospital
262 Danny Thomas Place
Memphis, TN 38105-3678
Email: jie.zheng@stjude.org
Phone: (901) 595-3168
FAX: (901) 595-3032


BS - Nankai University, Tianjin, China
PhD - City University of New York

Research Interests


Selected Publications

Rana R, Carroll CE, Lee HJ, Bao J, Marada S, Grace CRR, Guibao CD, Ogden SK*, Zheng JJ*. Structural insights into the role of the Smoothened cysteine rich domain in Hedgehog signaling. Nat Commun 4:2965, 2013.

Bao J, Lee HJ, Zheng JJ. Genome-wide network analysis of Wnt signaling in three pediatric cancers. Sci Rep 3:2969, 2013.

Cheepala SB, Bao J, Nachagari D, Sun D, Wang Y, Zhong T, Naren AP, Zheng J, Schuetz JD.  Crucial Role for Phylogenetically Conserved Cytoplasmic Loop 3 in ABCC4 Protein Expression. J Biol Chem 288:22207-18, 2013.

Shan J, Zheng JJ. Virtual ligand screening combined with NMR to identify Dvl PDZ domain inhibitors targeting the wnt signaling. Metheds Mol Biol 928:17-28, 2012.

Li X, Shan J, Chang W, Kim I, Bao J, Lee HJ, Zhang X, Samuel VT, Shulman GI, Liu D, Zheng JJ*, Wu D*. Chemical and genetic evidence for the involvement of Wnt antagonist Dickkopf2 in regulation of glucose metabolism. Proc Natl Acad Sci U S A 109:11402-7, 2012.

Bao J, Zheng JJ, Wu D. The structural basis of DKK-mediated inhibition of Wnt/LRP signaling. Sci Signaling 5(224), 2012.

Shan J, Zhang X, Bao J, Cassell R, Zheng JJ. Synthesis of Potent Dishevelled PDZ Domain Inhibitors Guided by Virtual Screening and NMR Studies. Chem Biol Drug Des 79:376-382, 2012.

Bari R, Guo Q, Xia B, Zhang YH, Giesert EE, Levy S, Zheng JJ, Zhang XA. Tetraspanins regulate the protrusive activities of cell membrane. Biochem Biophys Res Commun 415:619-26, 2011.

Alli R, Zhang ZM, Nguyen P, Zheng JJ, Geiger TL. Rational design of T cell receptors with enhanced sensitivity for antigen. PloS One 6(3):e18027, 2011.

Lee HJ, Zhang X, Zheng JJ. Inhibiting the Wnt Signaling Pathway with Small Molecules. Targeting the Wnt Pathway in Cancer, Chapter 9, K.H. Goss and M. Kahn (eds.), 2011.

Lee HJ, Finkelstein D, Li X, Wu D, Shi, D-L, Zheng JJ. Identification of transmembrane protein 88 (TMEM88) as a dishevelled-binding protein. J Biol Chem 285:41549-56, 2010.

Lee HJ, Zheng JJ. PDZ domains and their binding partners: structure, specificity, and modification. Cell Commun Signal 8:8, 2010.

Lin SL, Li B, Rao S, Yeo EJ, Hudson TE, Nowlin BT, Pei H, Chen L, Zheng JJ, Carroll TJ, Pollard JW, McMahon AP, Lang RA, Duffield JS. Macrophage Wnt7b is critical for kidney repair and regeneration. Proc Natl Acad Sci U S A 107(9):4194-9, 2010.

Lee HJ, Wang NX, Shi D-L, Zheng JJ. Sulindac Inhibits Canonical Wnt Signaling by Blocking the PDZ Domain of Dishevelled. Angew Chem Int Ed 48:6448-52, 2009.

Grandy D, Shan J, Zhang X, Rao S, Akunuru S, Li H, Zheng Y, Alpatov I, Zhang XA, Lang R, Shi DL, Zheng JJ. Discovery and characterization of a small molecule inhibitor of the PDZ domain of dishevelled. J Biol Chem 284:16256-63, 2009.

Wang NX, Zheng JJ. Computational studies of H5N1 influenza virus resistance to oseltamivir. Protein Sci 18:707-15, 2009.

Simons M, Gault WJ, Gotthardt D, Rohatgi R, Klein TJ, Shao Y, Lee HJ, Wu AL, Fang Y, Satlin LM, Dow JT, Chen J, Zheng J, Boutros M, Mlodzik M. Electrochemical cues regulate assembly of the Frizzled/Dishevelled complex at the plasma membrane during planar epithelial polarization. Nat Cell Biol 11:286-94, 2009.

Lee HJ, Wang NX, Shao YM, Zheng JJ. Identification of Tripeptides Recognized by the PDZ domain of Dishevelled. Bioorg & Med Chem 17:1701-8, 2009. 10.1016/j.bmc.2008.12.060.

Bari R, Zhang YH, Zhang F, Wang NX, Stipp CS, Zheng JJ, Zhang XA. Transmembrane Interactions Are Needed for KAI1/CD82-mediated Suppression of Cancer Invasion and Metastasis. Am J Pathol 174: 647-60, 2009. PMID: 19116362.

Wu BN, Zhang YM, Rock CO, Zheng JJ. Structural modification of acyl carrier protein by butyryl group. Protein Sci 18: 240-6, 2009.

Shan J, Zheng JJ. Optimizing Dvl PDZ domain inhibitor by exploring chemical space. J Comput Aided Mol Des 23: 37-47, 2009.

Chen LJ, Wang K, Shao YM, Huang J, Li XF, Shan JF, Wu D, Zheng JJ. Structural insight into the mechanisms of Wnt signaling antagonism by Dkk. J Biol Chem 283:23364-23370, 2008. PMID: 18524778.

Wang K, Zhang YZ, Li XF, Chen LJ, Wang H, Wu JG, Zheng JJ, Wu D. Characterization of the Kremen-binding site on Dkk1 and elucidation of the role of Kremen in Dkk-mediated Wnt antagonism. J Biol Chem 283: 23371-23375, 2008.  PMID: 18502762.

Zhang ZM, Simmerman JA, Guibao CD, Zheng JJ. GIT1 paxillin-binding domain is a four-helix bundle and it binds to both paxillin LD2 and LD4 motifs. J Biol Chem 283:18685-18693, 2008. PMID: 18448431.

Wang NX, Lee HJ, Zheng JJ. Therapeutic Use of PDZ Protein-Protein Interaction Antagonism.  Drug News & Perspect 21:137-142, 2008.

Xing Y, Takemaru K, Liu J, Berndt JD, Zheng JJ, Moon RT, Xu W. Crystal structure of a full-length beta-catenin. Structure 16:478-487, 2008.

Bertolucci CM, Guibao CD, Zheng JJ. Phosphorylation of paxillin LD4 destabilizes helix formation and inhibits binding to focal adhesion kinase. Biochem 47: 548-554, 2008.

Fujii N, You L, Xu Z, He B, Mikami I, Uematsu K, Shan J, Zheng J, McCormick F, Jablons DM, Guy RK. Disrupting the Protein-Protein Interaction on the PDZ Domain of Dishevelled by A Small Molecule Suppresses Tumor Progression. Cancer Research 67:573-579, 2007.

Nassar N, Cancelas J, Zheng J, Williams DA, Zheng Y. Structure-function based design of small molecule inhibitors targeting rho family GTPases. Curr Top Med Chem 6:1109-16, 2006.

Liu J, Xing Y, Hinds TR, Zheng J, Xu X. The third 20 amino acid repeat is the tightest binding site of APC for ß-catenin. J Mol Biol 360:133-144, 2006.

Dickerson JB, Morgan MA, Mishra A, Slaughter CA, Morgan JI, Zheng J. The Influence of Phosphorylation on the Activity and Structure of the Neuronal IQ Motif Protein, PEP-19. Brain Res 1092:16-27, 2006.

Obenauer JC, Denson J,  Mehta JP, Su X, Mukatira S, Finkelstein DB, Xu X, Wang J,  Ma J, Fan Y, Rakestraw YM,  Webster RG, Hoffmann E, Krauss S, Zheng J, Zhang Z, Naeve CW. Large Scale Sequence Analysis of Avian Influenza Isolates. Science 311:1576-1580, 2006.

Akbar H, Williams DA, Zheng J, Zheng Y. Rational design and applications of a Rac GTPase-specific small molecule inhibitor. Method in Enzymology 406:554-565, 2006.

Last update: May 2014