Michael M. Meagher, PhD
Michael M. Meagher, PhD

Michael M. Meagher, PhD

Member, St. Jude Faculty

  • President, Children's GMP LLC
  • Vice President, Therapeutics Production and Quality



PhD – Iowa State University
MS – Iowa State University
BS – Colorado State University

Research Interests

  • Fermentation and downstream processing of recombinant proteins
  • cGMP manufacturing
  • cGMP facility design

Selected Publications

Meagher MM, Seravalli JG, Swanson ST, Ladd RG, Pal Khasa Y, Inan M, Harner JC, Johnson SK, Van Cott K, Lindsey C, Wannemacher R, Smith LA. Process development and cGMP manufacturing of a recombinant ricin vaccine: An effective and stable recombinant ricin a-chain vaccine—RVEc™. Biotechnol Progress 27(4):1036-1047, 2011.

Pal Khasa Y, Conrad S, Sengul M, Plautz S, Meagher MM, Inan M. Isolation of Pichia pastoris PIR genes and their utilization for cell surface display and recombinant protein secretion. Yeast 28(3):213-226, 2011.

Dux MP, Huang J, Barent R, Inan M, Swanson ST, Sinha J, Ross JT, Smith LA, Smith TJ, Henderson I, Meagher MM. Purification of a recombinant heavy chain fragment C vaccine candidate against botulinum serotype C neurotoxin [rBoNTC(Hc)] expressed in Pichia pastorisProtein Express & Purif 75(2):177-185, 2011.

Jacobs PP, Inan M, Festjens N, Haustraete J, Van Hecke A, Contreras R, Meagher MM, Callewaert N. Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate. Microbial Cell Factories 9:93, 2010.

Webb RP, Smith TJ, Wright PM, Montgomery VA, Meagher MM, Smith LA. Protection with recombinant Clostridium botulinum C1 and D binding domain subunit (Hc) vaccines against C and D neurotoxins. Vaccine 25(21):4273-4282, 2007.

Parampalli A, Eskridge K, Smith L, Meagher MM, Mowry MC, Subramanian A. Developement of serum-free media in CHO-DG44 cells using a central composite statistical design. Cytotechnology 54(1):57-68, 2007.

Sinha J, Inan M, Fanders S, Taoka S, Gouthro M, Swanson T, Barent R, Barthuli A, Loveless BM, Smith LA, Smith T, Henderson I, Ross J, Meagher MM. Cell bank characterization and fermentation optimization for production of recombinant heavy chain C-terminal fragment of botulinum neurotoxin serotype E (rBoNTE(Hc): Antigen E) by Pichia pastorisJournal of Biotechnology 127(3):462-474, 2007.

Inan M, Aryasomayajula D, Meagher M. Enhancement of Protein Secretion in Pichia pastoris by Overexpression of Protein Disulfide Isomerase. Biotechnol Bioeng 93(4):771-778, 2006.

Dux MP, Barent R, Sinha J, Gouthro M, Swanson T, Barthuli A, Inan M, Ross JT, Smith LA, Smith TJ, Webb R, Loveless B, Henderson I, Meagher MM. Purification and Scale up of a Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype E [rBoNTE(Hc)] in Pichia pastoris GS115. Protein Express Purif 45:357-367, 2006.

Inan M, Aryasomayajula D, Sinha J, Meagher MM. Enhancement of protein secretion in pichia pastoris by overexpression of protein disulfide isomerase. Biotechnology and Bioengineering 93(4):771-778, 2006.

Zhang W, Sinha J, Meagher MM. Glycerophosphate as a phosphorus source in a defined medium for Pichia pastoris fermentation. Applied Microbiology and Biotechnology 72(1):139-144, 2006.

Zhang W, Sinha J, Smith LA, Inan M, Meagher MM. Maximization of Production of Secreted Recombinant Proteins in Pichia pastoris Fed-Batch Fermentation. Biotechnology Progress 21(2):386-393, 2005.

Asojo OA, Loukas A, Inan M, Barent R, Huang J, Plantz B, Swanson A, Gouthro M, Meagher MM, Hotez PJ. Crystallization and preliminary X-ray analysis of Na-ASP-1, a multi-domain pathogenesis-related-1 protein from the human hookworm parasite Necator americanus. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, F61(4):391-394, 2006.

Sinha J, Plantz BA, Inan M, Meagher M. Causes of Proteolytic Degradation of Secreted Recombinant Proteins Produced in Methylotrophic Yeast Pichia pastoris: Case Study with Recombinant Ovine Interferon-tau. Biotechnol Bioeng 89(1):102-12, 2005.

Mowry M, Meagher M, Smith L, Marks, Subramanian JA. Production and Purification of a Chimeric Monoclonal Antibody Against Botulinum Neurotoxin Serotype A. Protein Expr Purif 37(2):399-408, 2004.

Zhang W, Liu CP, Inan M, Meagher M. Optimization of Cell Density and Dilution Rate in Pichia pastoris Continuous Fermentations for Production of Recombinant Proteins. J Ind Microbiol Biotechnol 31(7):330-4, 2004.

Plantz BA, Andersen J, Smith LA, Meagher MM, Schlegel VL. Detection of Non-Host Viable Contaminants in Pichia pastoris Cultures and Fermentation Broths. J Indust Microbiol Biotechnol 30(11):643-650, 2003.

Hotez PJ, Zhan B, Bethony JM, Loukas A, Williamson A, Goud GN, Hawdon JM, Dobardzic A, Dobardzic R, Ghosh K, Bottazzi ME, Mendez S, Zook B, Wang Y, Liu S, Essiet-Gibson I, Chung-Debose S, Xiao S, Knox D, Meagher M, Inan M, Correa-Oliveira R, Vilk P, Shepherd HR, Brandt W, Russell PK. Progress in the development of a recombinant vaccine for human hookworm disease: The Human Hookworm Vaccine Initiative. International Journal for Parasitology 33(11):1245-1258, 2003.

Johnson SK, Zhang W, Smith LA, Hywood-Potter KJ, Swanson TS, Schlegel VL, Meagher M. Scale-up of the Fermentation and Purification of the Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype F Expressed in Pichia pastoris. Protein Express Purif 32(1):1-9, 2003.

Zhang W, Hywood Potter KJ, Plantz BA, Schlegel VL, Smith LA, Meagher MPichia pastorisFermentation with Mixed-Feeds of Glycerol and Methanol: Growth Kinetics and Production Improvement. J Industr Microbiol Biotechnol 30(4):210-215, 2003.

Sinha J, Plantz BA, Zhang W, Gouthro M, Schlegel VL, Liu C-P, Meagher M. Improved Production of Recombinant Ovine Interferon-tau by Mut+ Strain of Pichia pastoris Using an Optimized Methanol Feed Profile. Biotechnol Prog 19(3):794-802, 2003.

Plantz B, Andersen J, Smith L, Meagher M, Schlegel VL. Detection of Non-host Viable Contaminants in Pichia pastoris Cultures and Fermentation Broths. J Ind Microbiol Biotechnol 30(11):643-50, 2003.

Zhang W, Smith LA, Plantz BA, Schlegel VL, Meagher MM. Design of Methanol Feed Control in Pichia pastoris Fermentations Based upon a Growth Model. Biotechnol Prog 18:1392-9, 2002.

Inan M, Meagher M. Non-Repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris. J Biosci Bioeng 92:585-589, 2001.

Inan M, Meagher M. The effect of ethanol and acetate on protein expression in Pichia pastorisJ Biosci Bioeng 92:337-341, 2001.

Huang J, Meagher MM. Pervaporative recovery of butanol from aqueous solutions and ABE fermentation broth using thin-film silicalite-filled silicone composite membranes. J Membr Sci 192:231-242, 2001.

Qureshi N, Meagher MM, Huang J, Hutkins RW. Acetone butanol ethanol (ABE) recovery by pervaporation using silicalite-silicone composite membrane from fed-batch reactor of Clostridium acetobutylicumJ Membr Sci 187:93-102, 2001.

Fadeev AG, Selinskaya YA, Kelley SS, Meagher MM, Litvinova EG, Khotimsky VSVolkov VV. Extraction of butanol from aqueous solutions by pervaporation through poly[1-trimethylsilyl)-1-propyne]. J Membr Sci 186:205-217, 2001.

Fadeev AG, Meagher MM. Opportunities for ionic liquids in recovery of biofuels. Chem Commun (3):295-296, 2001.

Last update: September 2011