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Denis Noubouossie, MD, PhD
Denis Noubouossie, MD, PhD

Denis Noubouossie, MD, PhD

Assistant Member, St. Jude Faculty



MD – General Medicine, University of Yaoundé I, Cameroon
PhD (Biomedical Sciences) – Free University of Brussels, Belgium
Residency (Clinical Biology) – University of Yaoundé I, Cameroon
Post-graduate Fellow (Hematology and Hemostasis) – Teaching Hospital Brugmann, Brussels, Belgium
Postdoctoral Research Associate (Hematology) – School of Medicine, University of North Carolina, Chapel Hill, NC
Residency (Clinical Pathology) University of Alabama at Birmingham, Birmingham, AL
Fellowship (Blood Banking/Transfusion Medicine) – University of North Carolina, Chapel Hill, NC

Honors and Awards

  • 2022 Paul E. Standjord Young Investigator Program and William L. Roberts Award with Distinction for Excellence in Research. The Academy of Clinical Laboratory Physicians and Scientists annual meeting, Seattle
  • Alpha Omega Apha honor member
  • 2021-2022 Clinical Pathology Chief Resident, University of Alabama at Birmingham
  • 2015 American Society of Hematology Abstract Achievement Award
  • 2009-2013 Belgian Kid Funds for Research Award
  • 2010 Red Blood Cell and Iron Abstract Award for young scientists, Marseille, France

Research Interests

  • Abnormalities of coagulation in sickle cell disease
  • Procoagulant properties of cell-derived microvesicles
  • Procoagulant properties of components derived from the cell nucleus
  • Interactions between coagulation and inflammation

Selected Publications

DF Noubouossie and NS. Key: Red Cell Extracellular Vesicles and Coagulation Activation Pathways. Curr Opin Hematol Nov 1;30(6):194-202, 2023. PMID: 37548370 PMCID: PMC1052994.

Noubouossie DF, Henderson MW, Mooberry M, Ilich A, Ellsworth P, Piegore M, Skinner SC, Pawlinski R, Welsby I, Renné T, Hoffman M, Monroe DM, Key NS. Red blood cell microvesicles activate the contact system, leading to factor IX activation via 2 independent pathways. Blood Mar 5;135(10):755-765, 2020. PMID: 31971571

Noubouossie DF, Reeves BN, Strahl BD, Key NS. Neutrophils: back in the thrombosis spotlight. Blood May 16;133(20):2186-2197, 2019. PMID: 30898858

Noubouossie DF, Whelihan MF, Yu YB, Sparkenbaugh E, Pawlinski R, Monroe DM, Key NS. In vitro activation of coagulation by human neutrophil DNA and histone proteins but not neutrophil extracellular traps. Blood Feb 23;129(8):1021-1029, 2017. doi: 10.1182/blood-2016-06-722298.

Noubouossie DF, Key NS, Ataga KI. Coagulation Abnormalities of Sickle Cell Disease: Relationship with Clinical Outcomes and the Effect of Disease Modifying Therapies. Blood Rev Jul;30(4):245-56, 2016. doi: 10.1016/j.blre.2015.12.003. Review.

Noubouossie DF, Lê PQ, Ferster A, , Rozen L, Debaugnies F, Demulder A. Evaluation of the procoagulant activity of endogenous phospholipids in the platelet-free plasma of sickle cell disease children. Thrombosis Research 130(2):259-64, 2012.

Noubouossie DF, Lê PQ, Corazza F, Debaugnies F, Rozen L, Ferster A and Demulder A. Thrombin generation reveals a high haemostatic potential in the plasma of sickle cell disease children. American Journal of Hematology 87(2):145-149, 2012.

Noubouossie DF, Lê PQ, Rozen L, Ziereisen F, Willems D, Demulder A, Ferster A. Thrombin Generation in Children with Sickle Cell Disease: Relationship with Age, Hemolysis, Transcranial Doppler Velocity and Hydroxyurea Treatment. Eur J Haematol Jul;91(1):46-54, 2013.

Sui J, DF Noubouossie DF, Gandotra S, Cao L. Elevated Plasma Fibrinogen Is Associated With Excessive Inflammation and Disease Severity in COVID-19 Patients. Front Cell Infect Microbiol Aug 3;11:734005, 2021. doi: 10.3389/fcimb.2021.734005. PMID: 34414135

Godby RC, Kornbrust A, Noubouossie DF, Kanter J, Ogunsile J, Lima JLO, Singh N, Marques MB, Siniard RC. Adaptable Stewardship During a Pandemic: A Multidisciplinary Approach to Sustaining the Blood Supply for Patients with Sickle Cell Disease. Int J Lab Hematol Feb 12, 2021. doi: 10.1111/ijlh.13485. PMID: 33576556

Henderson MW, Sparkenbaugh EM, Wang S, Ilich A, Noubouossie DF, Mailer R, Renné T, Flick  MJ, Luyendyk JP, Chen Z-L, Strickland S, Stravitz RT, McCrae KR, Key NS, Pawlinski R. Plasmin-mediated Cleavage of High Molecular Weight Kininogen Contributes to Acetaminophen-Induced Acute Liver Failure. Blood Jul 22;138(3):259-272, 2021. doi: 10.1182/blood.2020006198. PMID: 33827130.

Ilich A, Kumar V, Ferrara MJ, Henderson MW, Noubouossie DF, Jenkins DH, Kozar RA, Park MS, Key NS. Euglobulin Clot Lysis Time Reveals a High Frequency of Fibrinolytic Activation in Trauma. Thromb Res Aug;204:22-28, 2021. doi: 10.1016/j.thromres.2021.05.017. Epub 2021 May 31. PMID: 34111811.

Thålin C, Aguilera K, Hall NW, Marunde MR, Burg JM, Rosell A, Daleskog M, Månsson M, Hisada Y, Meiners MJ, Sun ZW, Whelihan MF, Cheek MA, Howard SA, Saxena-Beem S, Noubouossie DF, Key NS, Sheikh SZ, Keogh MC, Cowles MW, Lundström S, Mackman N, Wallén H, Johnstone AL. Quantification of citrullinated histones: Development of an improved assay to reliably quantify nucleosomal H3Cit in human plasma. J Thromb Haemost Jul 12, 2020. PMID: 32654410

Henderson MW, Noubouossie DF, Ilich A, Jean WK, Pawlinski R, Monroe DM, Key NS. Protease:Serpin Complexes to assess Contact System and Intrinsic Pathway Activation. Res Pract Thromb Haemost Jun 25;4(5):789-798, 2020. PMID: 32685887

Hisada Y, Grover SP, Maqsood A, Houston R, Ay C, Noubouossie DF, Cooley BC, Wallén H, Key NS, Thålin C, Farkas ÁZ, Farkas VJ, Tenekedjiev K, Kolev K, Mackman N. Neutrophils and neutrophil extracellular traps enhance venous thrombosis in mice bearing human pancreatic tumors. Haematologica Jan;105(1):218-225, 2020. PMID: 31048354

Henderson M, Noubouossie DF, Ilich A, Jean WK, Pawlinski R, Monroe DM, Key NS. Protease:Serpin Complexes to assess Contact System and Intrinsic Pathway Activation. Res Pract Thromb Haemost Nov 6;4(1):46-53, 2019. doi: 10.1002/rth2.12275. eCollection 2020 Jan. 2019

Rozen L, Noubouossie DF, Dedeken L, Lê PQ, Ferster A, Demulder A. Is There Any Improvement of the Coagulation Imbalance in Sickle Cell Disease after Hematopoietic Stem Cell Transplantation? J Clin Med Oct 26;8(11):1796, 2019. PMID: 31717804

Ilich A, Noubouossie DF, Henderson M, Ellsworth P, Betbadal KF, Campello E, Meeks S, Dunn A, Park MS, Pawlinski R, Simioni P, Shapiro A, Key NS. Development and application of global assays of hyper- and hypofibrinolysis. Res Pract Thromb Haemost Nov 6;4(1):46-53, 2019. PMID: 31989084

Faes C, Ilich A, Sotiaux A, Sparkenbaugh EM, Henderson MW, Buczek L, Beckman JD, Ellsworth P, Noubouossie DF, Bhoopat L, Piegore M, Renoux C, Bergmeier W, Park Y, Ataga KI, Cooley B, Wolberg AS, Key NS, Pawlinski R. Red blood cells modulate structure and dynamics of venous clot formation in sickle cell disease. Blood Jun 6;133(23):2529-2541, 2019. PMID: 30952675

Campello E, Henderson MW, Noubouossie DF, Simioni P, Key NS. Contact System Activation and Cancer: New Insights in the Pathophysiology of Cancer-Associated Thrombosis. Thromb Haemost Feb;118(2):251-265, 2018. doi: 10.1160/TH17-08-0596. Epub 2018 Jan 29.

Rozen L, Noubouossie DF, Dedeken L, Huybrechts S, Lê PQ, Ferster A, Demulder A. Different profile of thrombin generation in children with acute lymphoblastic leukaemia treated with native or pegylated asparaginase: A cohort study. Pediatr Blood Cancer Feb;64(2):294-301, 2017. doi: 10.1002/pbc.26228.

Rozen L, Faraoni D, Torres CS, Willems A, Noubouossie DF, Barglazan D, Van der Linden P, Demulder A. Effective tranexamic acid concentration for 95% inhibition of tissue-type plasminogen activator induced hyperfibrinolysis in children with congenital heart disease: A prospective, controlled, in-vitro study. Eur J Anaesthesiol Aug 7, 2015. [Epub ahead of print]

Noubouossie DF, Key MS. Sickle cell disease and venous thromboembolism in pregnancy and the puerperium. Thromb Res Feb;135 (Suppl 1):S46-8, 2015.

Dedeken L, Chapusette R, Lê PQ, Heijmans C, Devalck C, Huybrechts S, Ziereisen F, Hanssens L, Rozen L, Noubouossie DF, Mujinga MN, Ferster A. Reduction of the six-minute walk distance in children with sickle cell disease is correlated with silent infarct: results from a cross-sectional evaluation in a single center in Belgium. PLoS One Oct 2;9(9):e108922, 2014.

Rozen L, Huybrechts S, Dedeken L, Heijmans C, Dessars B, Heimann P, Lambert F, Noubouossie DF, Ferster A, Demulder A. Transient leukemia in a newborn without Down syndrome: case report and review of the literature. Eur J Pediatr Dec;173(12):1643-7, 2014. 

Rozen L, Copette F, Noubouossie DF, A Demulder A. Evaluation of three APTT reagents in a routine laboratory: toward a compromise. Clin Lab 59(7-8):921-4, 2013.

Rozen L, Noubouossie DF, Demulder A. The place of D-dimers measurement and its improvements. Belgian Journal of Hematology 4(2):47-50, 2013.

Debaugnies F, Geneviève S, Badot V, Noubouossie DF, Willems D, Corazza F. Anti-cyclic citrullinated peptide antibodies, a comparison of different assays for the diagnosis of rheumatoid arthritis. Scand J Rheumatol 42(2):108-14, 2013.

Noubouossie DF, Corazza F, Valsamis J, Demulder A. A chemiluminescence immunoassay may detect mostly relevant IgG anticardiolipin antibodies according to revised Sydney criteria. Acta Clinica Belgica 67(3):184-189, 2012.

Noubouossie DF, Tayou Tagny C, Same-Ekobo A, Mbanya D. Asymptomatic carriage of malaria parasites in blood donors in Cameroon. Transfusion Medicine 22(1):63-67, 2012. 

Show More

Filippin L, Debaugnies F, Noubouossié DF, Lê PQ, Ferster A, Demulder A. Test de génération de thrombine : importance d’établir les valeurs de références en fonction de l’âge et des concentrations en facteur tissulaire avant l’implémentation au laboratoire. Rev Med Brux 32:69-73, 2011.

Debaugnies F, Azerad MA, Noubouossie DF, Rozen L, Hemker HC, Efira A, Demulder A. Evaluation of the procoagulant activity in the plasma of cancer patients using a thrombin generation assay. Thromb Res Dec;126(6):531-5, 2010.

Mesquita MCF, Bakoto ES, Malarme M, Noubouossie DF, Demulder A. Management of refractory essential thrombocythemia with anagrelide in a patient undergoing hemodyalisis. Clinical Therapeutics Nov;31:2559-64, 2009.

Noubouossie DF, Demulder A. Retrospective application of more standardised interpretations of normal plasma mixing studies in the lanboratory diagnosis of Lupus Anticoagulant. Acta Clinica Belgica 64(2):183, 2009.

Theunissens C, Janssens P, Demulder A, Noubouossie DF, Van Esbroeck M, Van Gompel A, Van Den Ende J. Falciparum malaria in patient 9 years after leaving malaria-endemic area [letter]. Emerg Infect Dis Jan;15(1):115-6, 2009.

Gonsu Fotsin J, Ongolo Zogo P, Noubouossie DF, Tagni Zukam D, Kamga JP, Fouda A, Bengono G. Profil scanographique de la pathologie naso-sinusienne à Yaoundé : à propos de 192 cas. Journal de Radiologie, Paris 2001.

Ongolo Zogo P, Ndjolo A, Noubouossie DF, A Fouda Onana, G Bengono, J Gonsu Fotsin. Tumeurs malignes des cavités naso-sinusiennnes en milieu africain : Etude clinique et scanographique de 29 cas. Journal de Radiologie Oct;82(10)1464, 2001.

Last update: April 2024