Shondra Pruett-Miller, PhD
Shondra Pruett-Miller, PhD

Shondra Pruett-Miller, PhD

Assistant Member, St. Jude Faculty

  • Director, Center for Advanced Genome Engineering

Departments

Education

PhD - University of Texas Southwestern Medical School, Dallas, Texas
BS
- University of Arkansas, Fayetteville, Arkansas

Research Interests

  • Genome-editing technologies

Selected Publications

Wen Y, Liao G, Pritchard T, Zhao TT, Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB.  A Stable but Reversible Integrated Surrogate Reporter for Assaying CRISPR/Cas9-Stimulated Homology-directed Repair. J Biol Chem Feb 22, 2017. pii: jbc.M117.777722. doi: 10.1074/jbc.M117.777722. [Epub ahead of print] PMID: 28228480

Cohen AJ, Saiakhova A, Corradin O, Luppino JM, Lovrenert K, Bartels CF, Morrow JJ, Mack SC, Dhillon G, Beard L, Myeroff L, Kalady MF, Willis J, Bradner JE, Keri RA, Berger NA, Pruett-Miller SM, Markowitz SD, Scacheri PC. Hotspots of aberrant enhancer activity punctuate the colorectal cancer epigenome. Nat Commun Feb 7;8:14400, 2017. doi: 10.1038/ncomms14400. PMID: 28169291

Orchard RC, Wilen CB, Doench JG, Baldridge MT, McCune BT, Lee YC, Lee S, Pruett-Miller SM, Nelson CA, Fremont DH, Virgin HW. Discovery of a proteinaceous cellular receptor for a norovirus. Science Aug 26; 353(6302):933-6, 2016. doi: 10.1126/science.aaf1220. Epub 2016 Aug 18. PubMed PMID: 27540007.

Redmann V, Lamb CA, Hwang S, Orchard RC, Kim S, Razi M, Milam A, Park S, Yokoyama CC, Kambal A, Kreamalmeyer D, Bosch MK, Xiao M, Green K, Kim J, Pruett-Miller SM, Ornitz DM, Allen PM, Beatty WL, Schmidt RE, DiAntonio A, Tooze SA, Virgin HW. Clec16a is Critical for Autolysosome Function and Purkinje Cell Survival. Sci Rep 6:23326, 2016. PMID: 26987296

Pruett-Miller SM, Davis GD. Donor plasmid design for codon and single base genome editing using zinc finger nucleases. Method Mol Bio 1239:219-29, 2015. PMID: 25408411

Chen F, Pruett-Miller SM, Davis GD. Gene editing using ssODNs with engineered endonucleases. Methods Mol Bio 1239:251-65, 2015. PMID: 25408408

Duda K, Lonowski LA, Kofoed-Nielsen M, Ibarra A, Delay CM, Kang Q, Yang Z, Pruett-Miller SM, Bennett EP, Wandall HH, Davis GD, Hansen SH, Frodin M. High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases of CRISPR/Cas9 nickase pairs. Nucleic Acids Research Apr 21, 2014. PMID: 24753413

Voit RA, Hendel A, Pruett-Miller SM, Porteus MH. Nuclease-mediated gene editing by homologous recombination of the human globin locus. Nucleic Acids Research Jan;42(2):1365-78, 2014. PMID: 24157834

Wilson KA, McEwen AE, Pruett-Miller SM, Zhang J, Kildebeck EJ, Porteus MH. Expanding the Repertoire of target sites for zinc finger nuclease-mediated genome modification. Molecular Therapy Nucleic Acids 2:e88, 2013. PMID: 23632390

Chen F, Pruett-Miller SM, Huang Y, Gjoka M, Duda K, Taunton J, Frodin M, Davis GD. High Frequency Genome Editing Using ssDNA with Zinc Finger Nucleases. Nature Methods 753-755, 2011. PMID: 21765410

Davis GD, Pruett-Miller SM. ZFN Donor Design; Codon and Single Base Genome Editing Using Zinc Finger Nucleases. Biowire 39-43, 2010.

Connelly JP, Barker JC, Pruett-Miller S, Porteus MH. Gene Correction by Homologous Recombination with Zinc Finger Nucleases in Primary Cells from a Mouse Model of a Generic Recessive Genetic Disease. Molecular Therapy 1103-10, 2010. PMID: 20389291

Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, Cheng L. Gene Targeting of a Disease-related Gene in Human Induced Pluripotent Stem and Embryonic Cells. Cell Stem Cell 97-110, 2009. PMID: 19540188

Pruett-Miller SM, Reading D, Porter SN, Porteus MH. Attenuation of Zinc Finger Nuclease Cytotoxicity by Regulating Protein Expression. PLoS Genetics e1000376, 2009. PMID: 19214211

Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, Eichtinger M, Jiang T, Foley JE, Winfrey RF, Townsend JA, Unger-Wallace E, Sander JD, Muller-Lerch Felix, Pearlberg J, Gobel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK. Efficient Gene Modification Enabled by an OPEN (Oligomerized Pool ENgineering) Platform for Constructing Zinc Finger Nucleases. Molecular Cell 294-301, 2008. PMID: 18657511

Pruett-Miller SM, Connelly JP, Maeder ML, Joung JK, Porteus MH. Optimizing Zinc Finger Nucleases for Gene Targeting in Mammalian Cells. Molecular Therapy 707-717, 2008. PMID: 18334988

Porteus MH, Connelly JP, Pruett SM. A Look to the Future Directions in Gene Therapy Research for Monogenic Diseases. PLOS Genetics 1285-1292, 2006. PMID: 17009872

Last update: February 2017