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Marcus Fischer, PhD
Marcus Fischer, PhD

Marcus Fischer, PhD

Associate Member, St. Jude Faculty

  • Adjunct Assistant Professor at the University of Tennessee Health Science Center (Department of Pharmaceutical Sciences)



PhD - University of York, York, UK
MSc - University of Lübeck, Lübeck, Germany
BSc - University of Lübeck, Lübeck, Germany

Research Interests

  • Chemical biology
  • Ligand discovery
  • Structural biology
  • Variable-temperature protein crystallography
  • Protein conformational ensembles
  • Protein flexibility and hydration
  • Protein structure – (mal-)function
  • Molecular docking

Selected Publications

Stachowski TR, Fischer M.#. Large-scale ligand perturbations of the protein conformational landscape reveal state-specific interaction hotspots. J Med Chem 10.1021/acs.jmedchem.2c00708, 2022.

Stachowski TR, Vanarotti M, Seetharaman J, Lopez K., Fischer M.#. Water Networks Repopulate Protein-Ligand Interfaces with Temperature. Angewandte Chemie International Edition 71, e202112919, 2022.

Bradford SYC, El Khoury L, Ge Y, Osato M, Mobley DL, Fischer M #. Temperature artifacts in protein structures bias ligand-binding predictions. Chemical Science 12, 11275-11293, 2021.

Min J*, Mayasundari A*, Keramatnia Fˇ, Jonchere B*, Yang SW, Jarusiewicz JA, Actis M, Das S, Young BM, Slavish PJ, Yang L, Li Y, Fu X, Garrett SH, Yun M, Nithianantham S, Chai SC, Chen T, Shelat AA, Lee RE, Nishiguchi G, White SW, Roussel MF, Potts PR, Fischer M, Rankovic Z. Phenyl-Glutarimides: Alternative Cereblon Binders for the Design of PROTACs with Improved Chemical Stability and Degradation Efficiency. Angewandte Chemie, 2021. DOI: 10.1002/anie.202108848

Zahid H*, Buchholz CR*, Singh M, Chan A, Nithianantham S, Shi K, Aihara H, Fischer M, Schonbrunn E, Landry JW, Pomerantz WCK. New design rules for developing potent, cell-active inhibitors of the Nucleosome Remodeling Factor (NURF) via BPTF bromodomain inhibition. J Med Chem, 2021. DOI: 10.1021/acs.jmedchem.1c01294

Chang Y*, Min J*, Jarusiewicz JA, Actis M, Yu S, Mayasundari A, Yang L, Chepyala D, Alcock L, Roberts KG, Nithianantham S, Maxwell D, Rowland L, Larsen R, Seth A, Goto H, Imamura T, Akahane K, Hansen BS, Pruett-Miller SM, Paietta E, Litzow MR, Qu C, Yang JJ, Fischer M#, Rankovic Z#, Mullighan CG#. Degradation of Janus kinases in CRLF2-rearranged acute lymphoblastic leukemia. Blood, 2021. DOI: 10.1182/blood.2020006846

Nishiguchi G*, Keramatnia F*, Min J*, Price J, Chepyala D, Young B, McGowan K, Slavish PJ, Mayasundari A, Jarusiewicz JA, Actis M, Yang L, Li Y, Fu X, Garrett SH, Das S, Chang Y, Jonchere B, Papizan JB, Kodali K, Peng J, Pruett-Miller SM, Roussel MF, Mullighan C, Fischer M, Rankovic, Z.. A focused library of Cereblon modulators uncovers potent, selective and orally bioavailable GSPT1/2 degraders. J Med Chem 64, 7296–7311, 2021.

Nicholls RA, Joosten RP, Long F, Wojdyr M, Lebedev A, Krissinel E, Catapano L, Fischer M, Emsley P, Murshudov GN. Modelling Covalent Linkages in CCP4. Acta Cryst D 77, 712-726, 2021.

Nicholls RA, Wojdyr M, Joosten RP, Long F, Lebedev A, Catapano L, Fischer M, Emsley P, Murshudov GN. The Missing Link – Covalent Linkages in Structural Models. Acta Cryst D 77, 727-745, 2021.

Fischer M. Macromolecular room temperature crystallography. Quarterly Reviews of Biophysics 54: e1, 2021.

Darby JF, Hopkins AP, Shimizu S, Roberts SM, Brannigan JA, Turkenburg JP, Thomas GH, Hubbard RE, Fischer M#. Water networks can determine the affinity of ligand binding to proteins. JACS doi: 10.1021/jacs.9b06275, 2019.

Pottel J, Levit A, Korczynska M, Fischer M, Shoichet BK. The Recognition of Unrelated Ligands by Identical Proteins. ACS Chem Bio 13:2522-2533, 2018.

Balius TE*, Fischer M*, Stein RM, Adler TB, Nguyen CN, Cruz A, Gilson MK, Kurtzman T, Shoichet BK. Testing Inhomogeneous Solvation Theory in Structure-Based Ligand Discovery. PNAS 114:E6839–E6846, 2017.

Lee H, Fischer M, Shoichet BK, Liu S-Y. Hydrogen Bonding of 1,2-Azaborines in the Binding Cavity of T4 Lysozyme Mutants: Structures and Thermodynamics. JACS 138:12021-12024, 2016.

Fischer M, Shoichet BK, Fraser JS. One crystal, two temperatures – Cryocooling Penalties alter Ligand Binding to Transient Protein Sites. ChemBioChem 16:1560-1564, 2015.

Merski M*, Fischer M*, Balius TE*, Eidam O, Shoichet BK. Homologous ligands accommodated by discrete conformations of a buried cavity. PNAS 112:5039-5044, 2015.

Fischer M, Hopkins AP, Severi E, Hawkhead J, Bawdon D, Watts AG, Hubbard RE, Thomas GH. Tripartite ATP-independent periplasmic (TRAP) transporters use an arginine-mediated selectivity filter for high affinity substrate binding. J Biol Chem 290:27113-27123, 2015.

Fischer A, Fischer M, Nicholls RA, Lau S, Poettgen J, Patas K, Heesen C, Gold S. Diagnostic accuracy for major depression in multiple sclerosis using self-report questionnaires. Brain and Behavior 5:e00365, 2015.

Nicholls RA, Fischer M, McNicholas S, Murshudov GN. Conformation-independent structural comparison of macromolecules with ProSMART. Acta Cryst D 70:2487-2499, 2014.

Fischer M*, Coleman RG*, Fraser JS, Shoichet BK. Incorporating protein flexibility and conformational energy penalties in docking screens to improve ligand discovery. Nature Chem 6:575-583, 2014.

Rocklin GJ*, Boyce SE*, Fischer M*, Fish I, Mobley DL, Shoichet BK, Dill KA. Blind prediction of charged ligand binding affinities in a model binding site. J Mol Biol 25:4569-4583, 2013.

Barelier S, Boyce SE, Fish I, Fischer M, Goodin DB, Shoichet BK. Roles for Ordered and Bulk Solvent in Ligand Recognition and Docking in Two Related Cavities. PLoS One 8:e69153, 2013.

Nicholls R A, Fischer M, Murshudov GN. An Overview of ProSMART. CCP4 Newsletter on Protein Crystallography 48, 2012.

Fischer M, Leech AP, Hubbard RE. Comparative Assessment of Different Histidine Tags for Immobilization of Protein onto Surface Plasmon Resonance Sensorchips. Anal Chem 83:1800-1807, 2011.

Mulligan C, Fischer M, Thomas, GH. Tripartite ATP-independent periplasmic (TRAP) transporters in bacteria and archaea. FEMS Microbiol Rev 35:68-86, 2011.

Fischer M, Zhang QY, Hubbard RE, Thomas GH. Caught in a TRAP: substrate binding proteins in secondary transport. Trends Microbiol 18:471-478, 2010.

Fischer M, Hubbard RE. Fragment-based ligand discovery. Mol Interv 9:22-30, 2009.

Last update: September 2022