Junmin Peng, PhD
Junmin Peng, PhD

Junmin Peng, PhD

Member, St. Jude Faculty

  • Director, Proteomics Facility

Departments

Education

BS – Wuhan University, Wuhan, China
PhD – University of Iowa
Postdoctoral Fellowship – Harvard Medical School

Research Interests

  • Mass Spectrometry-based Proteomics and Metabolomics
  • Systems Biology of Cancer
  • Molecular Pathogenesis of Alzheimer’s Disease
  • Ubiquitin and Proteome Turnover

Our mission is to develop novel mass spectrometry-based proteomics and metabolomics technologies, systems biology approaches, and to apply these tools to biomedical challenges (e.g. Alzheimer’s disease and cancer). We seek to profile the full spectra of temporal omics data including protein modifications (e.g. phosphoproteome and ubiquitinome) from cellular and animal models as well as human clinical specimens. Network analysis and integration of such large-scale omics data with genome, transcriptome, interactome and phenotypic information offer a systems or holistic view, for unbiased identification of central disease gene/protein networks, functional modules and master regulators. Beyond big data analysis, we perform a series of functional experiments to validate the derived hypotheses. These studies provide novel insights into the pathogenesis for therapeutic intervention, and may discover disease biomarkers for precision medicine.

Selected Publications

See Dr. Peng's citations on Google Scholar.

Wang X, Pandey AK, Mulligan MK, Williams EG, Mozhui K, Li Z, Jovaisaite V, Quarles LD, Xiao Z, Huang J, Capra JA, Chen Z, Taylor WL, Bastarache L, Niu X, Pollard KS, Ciobanu DC, Reznik AO, Tishkov AV, Zhulin IB, Peng J, Nelson SF, Denny JC, Auwerx J, Lu L, Williams RW. Joint mouse-human phenome-wide association to test gene function and disease risk. Nat Commun 7:10464, 2016.

Gao Y, Li Y, Zhang C, Zhao M, Deng C, Lan Q, Liu Z, Su N, Wang J, Xu F, Xu Y, Ping L, Chang L, Gao H, Wu J, Xue Y, Deng Z, Peng J, Xu P. Enhanced Purification of Ubiquitinated Proteins by Engineered Tandem Hybrid Ubiquitin-binding Domains (ThUBDs). Mol Cell Proteomics 15:1381-96, 2016.

Churchman ML, Low J, Qu C, Paietta EM, Kasper LH, Chang Y, Payne-Turner D, Althoff MJ, Song G, Chen SC, Ma J, Rusch M, McGoldrick D, Edmonson M, Gupta P, Wang YD, Caufield W, Freeman B, Li L, Panetta JC, Baker S, Yang YL, Roberts KG, McCastlain K, Iacobucci I, Peters JL, Centonze VE, Notta F, Dobson SM, Zandi S, Dick JE, Janke L, Peng J, Kodali K, Pagala V, Min J, Mayasundari A, Williams RT, Willman CL, Rowe J, Luger S, Dickins RA, Guy RK, Chen T, Mullighan CG. Efficacy of Retinoids in IKZF1-Mutated BCR-ABL1 Acute Lymphoblastic Leukemia. Cancer Cell 28:343-56, 2015.

Martinez J, Malireddi RK, Lu Q, Cunha LD, Pelletier S, Gingras S, Orchard R, Guan JL, Tan H, Peng J, Kanneganti TD, Virgin HW, Green DR. Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins. Nat Cell Biol 17:893-906, 2015.

Tan H, Wu Z, Wang H, Bai B, Li Y, Wang X, Zhai B, Beach TG, Peng J. Refined phosphopeptide enrichment by phosphate additive and the analysis of human brain phosphoproteome. Proteomics 15:500-7, 2015

Wang X, Li Y, Wu Z, Wang H, Tan H and Peng J. JUMP: a tag-based database search tool for peptide identification with high sensitivity and accuracy. Mol Cell Proteomics 13:3663-73, 2014.

Jones DR, Wu Z, Chauhan D, Anderson KC, Peng J. A nano Ultra-Performance Liquid Chromatography - High Resolution Mass Spectrometry Approach for Global Metabolomic Profiling and Case Study on Drug-Resistant Multiple Myeloma. Anal Chem 86:3667-75, 2014.

Bai B, Hales CM, Chen PC, Gozal Y, Dammer EB, Fritz JJ, Wang X, Xia Q, Duong DM, Street C, Cantero G, Cheng D, Jones DR, Wu Z, Li Y, Diner I, Heilman CJ, Rees HD, Wu H, Lin L, Szulwach KE, Gearing M, Mufson EJ, Bennett DA, Montine TJ, Seyfried NT, Wingo TS, Sun YE, Jin P, Hanfelt J, Willcock DM, Levey A, Lah JJ, Peng J. U1 Small Nuclear Ribonucleoprotein Complex and RNA Splicing Alterations in Alzheimer’s Disease. Proc Natl Acad Sci U S A. 110:16562-7, 2013.

Willmund F, Del Alamo M, Pechmann S, Chen T, Albanèse V, Dammer EB, Peng J, Frydman J. The cotranslational function of ribosome-associated hsp70 in eukaryotic protein homeostasis. Cell 152:196-209, 2013.

Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J. Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis J Proteome Res 11:4722-32, 2012.

Dammer EB, Na CH, Xu P, Seyfried NT, Duong DM, Cheng D, Gearing M, Rees H, Lah JJ, Levey AI, Rush J, Peng J. Polyubiquitin linkage profiles in three models of proteolytic stress suggest etiology of Alzheimer disease. J Biol Chem 286:10457-65, 2011.

Bingol B, Wang CF, Arnott D, Cheng D, Peng J, Sheng M. Autophosphorylated CaMKIIalpha acts as a scaffold to recruit proteasomes to dendritic spines. Cell 140:567-578, 2010.

Xu P, Duong DM, Seyfried NT, Cheng D, Xie Y, Robert J, Rush J, Hochstrasser M, Finley D, Peng J. Quantitative proteomics reveals the function of unconventional ubiquitin chains in proteasomal degradation. Cell 137:133-145, 2009.

Liu Y, Ye X, Jiang F, Liang C, Cheng D, Peng J, Kinch L, Grishin N, Liu Q. C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation. Science 325:750-753, 2009.

Qi HH, Ongusaha PP, Myllyharju J, Cheng D, Pakkanen O, Shi Y, Lee SW, Peng J, Shi Y. Prolyl 4-hydroxylation regulates Argonaute 2 stability. Nature 455:421-4, 2008.

Choe EA, Liao L, Zhou, JY, Cheng D, Duong DM, Jin P, Tsai LH, Peng J. Neuronal morphogenesis is regulated by the interplay between cyclin-dependent kinase 5 and the ubiquitin ligase mind bomb 1. J Neurosci 27:9503-9512, 2007.

Cheng D, Hoogenraad CC, Rush J, Ramm E, Schlager MA, Duong DM, Xu P, Wijayawardana SR, Hanfelt J, Nakagawa T, Sheng M, Peng J. Relative and absolute quantification of postsynaptic density proteome isolated from rat forebrain and cerebellum. Mol Cell Proteomics 5:1158-1170, 2006.

Liao L, Cheng D, Wang J, Duong DM, Losik TG, Gearing M, Rees HD, Lah JJ, Levey AI, Peng J. Proteomic characterization of postmortem amyloid plaques isolated by laser capture microdissection. J Biol Chem 279:37061-37068, 2004.

Peng J, Elias JE, Schwartz D, Thoreen CC, Cheng D, Marshiscky G, Roelofs J, Finley D, and Gygi SP. A proteomics approach to protein ubiquitination. Nat Biotechnol 21:921-926, 2003.

Peng J, Elias JE, Thoreen CC, Licklider LJ, Gygi SP. Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for large-scale protein analysis: the yeast proteome. J Proteome Res 2:43-50, 2003.

Lee MS, Kwon YT, Li M, Peng J, Friedlander RM, Tsai LH. Neurotoxicity induces calpain cleavage of p35 to p25. Nature 405:360-4, 2000.

Peng J, Zhu Y, Milton J, Price DH. Identification of multiple cyclin subunits of human P-TEFb. Genes Dev 12:755-62, 1998.

Last update: June 2016