Human iPSC Core

The Hematology Human Induced Pluripotent Stem Cell (iPSC) Core provides comprehensive, collaborative expertise in human pluripotent stem cell biology, genome engineering, disease modeling and hematopoietic differentiation. The Core partners with investigators to generate, characterize, genetically modify, maintain and differentiate human iPSC lines in support of basic, translational and disease-focused research.

The Core has extensive expertise in directing iPSCs toward hematopoietic lineages. Differentiation capabilities include the generation of megakaryocytes, erythroid cells, myeloid cells, B cells, NK cells, and hematopoietic stem and progenitor cells (HSPCs), including transplantable HSPC differentiation. The Core also supports the generation of iPSC-derived bone marrow organoids and other advanced cellular models designed to better recapitulate the hematopoietic and bone marrow microenvironment.

In addition to differentiation services, the Core provides iPSC reprogramming, including nonintegrating LNP-mRNA reprogramming, genome editing, clonal expansion, quality control, characterization, banking and iPSC line distribution.

The Core also provides hands-on training and consultation for investigators who wish to establish or expand iPSC-based approaches in their own laboratories. For studies of inherited predisposition syndromes and other disorders with phenotypes extending beyond the hematopoietic system, the Core offers expertise in differentiating iPSCs into additional lineages, including neural and cardiac cell types.

The Hematology iPSC Core provides customized, collaborative support for iPSC generation, characterization, genome engineering, and differentiation. Services include:

• iPSC reprogramming, including nonintegrating LNP-mRNA–based reprogramming
• iPSC culture, expansion, banking and distribution
• iPSC characterization and quality control
• Genome editing and clonal isolation
• Hematopoietic differentiation. Examples include:
  • HSPCs, including primitive and transplantable HSPC differentiation
  • Megakaryocytes
  • Erythroid cells
  • Myeloid cells
  • B cells
  • NK cells
• Bone marrow organoid differentiation and advanced hematopoietic models
• Neural and cardiac differentiation for selected projects involving non-hematopoietic phenotypes
• Protocol development, consultation and hands-on training for investigators working with human iPSCs

Lei Han, PhD
Lead Scientist

Hematology Human iPSC Core
262 Danny Thomas Pl
Room D3024
Memphis TN 38105-3678
USA