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Center for Proteomics and Metabolomics

The interplay of mass spectrometry-based proteomic, metabolomic and lipidomic technologies within the Center for Proteomics and Metabolomics makes it highly adaptable to take on any project.

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OVERVIEW

The mission of the Center for Proteomics and Metabolomics is to provide mass spectrometry-based protein, metabolomic and lipidomic technologies to St. Jude researchers including simple protein characterization, posttranslational modification analysis, comprehensive (phospho)proteome analysis, metabolite and lipid profiling. The interplay of technologies within the same group makes the center highly adaptable to take on any project. Extensive consultation is provided for experimental design, sample preparation, and MS data acquisition and interpretation. The Center has over 2200 square feet of space dedicated for research and instrumentation, and is supported by 13 full time employees.

IMPACT

The Center enables investigators at St Jude to explore all areas of protein and metabolite research.  The Center is constantly evolving and offering new services to adapt to the changing needs of its users.  Because of the extensive research and development activities, including 4 dedicated bioinformaticians building software, there is no need to wait for commercial protocols and solutions to be created. This facilitates access to techniques and data that do not exist elsewhere. 

Proteomics has been a really important core for us. They will meet with trainees to make sure the experiments are set up correctly. If there are new techniques that could be used to address the questions, they bring those on. It’s so great to have these resources in house.

Mondira Kundu, Member

Services and technologies

Intact Mass Measurement – analyze the intact mass of highly purified proteins
Protein ID by (Rapid) LC-MS/MS analysis – determine the identity of abundant proteins from gel bands
Posttranslational modification analysis – analyze protein posttranslational modifications on highly purified proteins
Large-scale protein profiling by label-free, SILAC, or TMT methods
Phosphoproteome profiling by SILAC or TMT – identify and quantify phosphoproteins
Metabolome profiling by LC-MS/MS - unbiased label free method to identify and quantify metabolites
Lipidome profiling by LC-MS/MS - unbiased label free method to identify and quantify lipids
Custom MS Analysis and additional bioinformatic analysis
JUMP – all mass spectrometry raw data are processed by our sophisticated in-house JUMP software suite

Instrumentation

(2) Thermo Q Exactive HF MS
(1) Thermo Orbitrap Fusion Tribrid MS
(2) Thermo Orbitrap Exploris 480 MS
(1) Bruker timsTOF SCP MS
(1) Bruker timsTOF HT MS

By the numbers

139

139 investigator collaborations

By the numbers

25

25 department collaborations

By the numbers

120

120 peer-reviewed publication contributions

Director

Andy High, PhD

Director, Center for Proteomics and Metabolomics

anthony.high@stjude.org