Scientists at St. Jude Children’s Research Hospital have created a tool that can find safe places to introduce genes into human DNA. The tool is an early step in the process to improve the safety and efficacy of gene and cell therapies. The work appears today in Genome Biology.
“We’ve created the Google Maps of editing the genome,” said co-corresponding author Yong Cheng, Ph.D., St. Jude Department of Hematology. “With this tool, we provide a new approach to identify places to safely integrate a gene cassette. We created step-by-step directions, so you can follow the steps and easily find safe harbor sites in specific tissues.”
Gene therapy, where a patient is given a functional copy of a dysfunctional gene, has shown success in curing certain genetic disorders. However, the field has encountered safety issues, including the inadvertent activation of an oncogene that led to cancer in some patients. In response, the field has searched for “safe harbor sites” – places in the genome where a gene can be inserted without causing cancer or other problems. The scientists created a pipeline that uses genomic and epigenetic information from specific tissue, such as blood cells, to find safe harbor sites.
A novel way to find safe harbor sites
The tool compares the DNA sequences that are highly variable between healthy people, using data from the 1000 Genomes Project. If a region of DNA is often deleted or inserted in healthy people, the researchers reasoned that it could likely also be altered safely by a gene therapy.
“Our method is a new way to identify genomic safe harbor sites in a tissue-specific manner,” Cheng said. “Nobody has tried it from this angle. Our first step was to find the genomic loci that show a high frequency of insertion or deletion among healthy individuals.”
If DNA in a single cell was a string, it would be two meters long. But in addition to the linear sequence, DNA can loop into complex 3D structures using chromatin, the proteins associated with DNA, to fit within a cell. Just like a string, DNA can have loops that affect its function. The St. Jude tool considers the presence of these loops and other structures when searching for accessible safe harbor sites.
“Our tool assesses the 3D structure of DNA, because human DNA is not a one-dimensional linear structure, it's actually 3D,” Chen said. “So, parts of DNA may be far away in the linear sequence of DNA but may physically be next to each other because of the looping of the 3D structure. In that case, the 3D proximity is more important than the linear distance.”
Balancing safety and therapeutic gene expression
“Safe gene therapy requires two things,” said Cheng. “Number one, maintaining high expression of the new gene. And number two, the integration needs to have minimal effects on the normal human genome, which is a major concern for people performing gene therapy.”
The scientists found that the genes placed in safe harbor sites identified by their tool maintained their expression over time. The researchers also showed that if they put a gene into one of the safe harbor sites identified by their tool, it affected nearby genes less than a classic safe harbor site.
The tool, Genomics and Epigenetic Guided Safe Harbor mapper (GEG-SH mapper), is freely available on Github.
Authors and funding
The study’s first authors are Dewan Shrestha, of St. Jude and The University of Tennessee Health Science Center, and Aishee Bag, of Rutgers, The State University of New Jersey. The study’s other authors are
Ruiqiong Wu, Xing Tang and Qian Qi, of St. Jude; Yeting Zhang and co-corresponding author Jinchuan Xing, of Rutgers, The State University of New Jersey.
The study was supported by grants from the National Cancer Institute (P30 CA021765), the National Institutes of Health (R35GM133614), St. Jude Collaborative Research Consortium on Novel Gene Therapies for Sickle Cell Disease (SCD), Human Genetics Institute of New Jersey and ALSAC, the fundraising and awareness organization of St. Jude.
St. Jude Children's Research Hospital
St. Jude Children's Research Hospital is leading the way the world understands, treats and cures childhood cancer and other life-threatening diseases. It is the only National Cancer Institute-designated Comprehensive Cancer Center devoted solely to children. Treatments developed at St. Jude have helped push the overall childhood cancer survival rate from 20% to 80% since the hospital opened more than 60 years ago. St. Jude freely shares the breakthroughs it makes, and every child saved at St. Jude means doctors and scientists worldwide can use that knowledge to save thousands more children. To learn more, visit stjude.org or follow St. Jude on social media at @stjuderesearch.