High Throughput Bioscience (HTB) Center

The High Throughput Bioscience (HTB) center was founded in 2006 (initially as the High Throughput Screening Center). The mission of HTB is multi-pronged, with the goals of 1) identifying novel chemical probes by large-scale HTS and high-content screening (HCS) of the St. Jude Compound Collection and 2) revealing novel gene function (by genome wide siRNA screening) to enable investigation of previously unknown mechanisms that explain physiological and pathological processes and discover novel therapeutics to treat diseases.

Experts in HTB use cutting-edge assay development technologies, sophisticated fully integrated robotics systems, and large-scale data processing and visualization tools to enable the chemical biology aspectis of collaborators' research and advance the mission of the institution. HTB collaborates with St. Jude investigators to develop assays for HTS/HCS and validate them, so they are amenable to automation. After a screen is designed, HTB implements projects, coordinates and executes the appropriate secondary assays, and provides screening support to identify SAR effort. To ensure the resources are allocated efficiently and fairly, potential collaborative projects are first reviewed for scientific rigor, technical feasibility, and alignment with institutional goals.

In addition, HTB manages the St. Jude Compound Collection in conjunction with other CBT centers. HTB develops and implements novel drug-discovery technologies, reagents, analytical tools, and data analysis pipelines. We also participate in large grant (U and P) applications, provide support to St. Jude investigators for their grant applications, and offer necessary training to the research community.