^{Chemical Biology and Therapeutics}

High Throughput Bioscience (HTB) Center

A collaborative center for assay development and high throughput screening in all phases of project conception, development, validation, screening, and post-screen hit validation.

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About the Center

The High Throughput Bioscience (HTB) center consists of qualified members with advanced biology, chemistry, and engineering training. We have experience in target identification and validation, assay development, high throughput screening, high content screening, laboratory automation, and management of scientific collaborations. We assist our collaborators in assay development, assay validation, miniaturization, and automation. We implement and manage HTS projects; manage and utilize whole genome siRNA libraries (human and mouse); evaluate and develop novel drug discovery technologies; and provide training to the St. Jude community. The following overview can represent the collaborative process:

Publications

Development of BODIPY FL Thalidomide As a High Affinity Fluorescent Probe for Cereblon in a Time-Resolved Fluorescence Resonance Energy Transfer Assay

Lin W et al. Bioconjug Chem, 2020

Development of BODIPY FL VH032 as a High-Affinity and Selective von Hippel−Lindau E3 Ligase Fluorescent Probe and Its Application in a Time-Resolved Fluorescence Resonance Energy-Transfer Assay

Lin W et al. ACS Omega, 2020

A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery

Chen T

Assay validation in high throughput screening - from concept to application

Chai SC et al. IntechOpen, 2015

Practical considerations of liquid handling devices in drug discovery

Chai SC et al. IntechOpen, 2013

Data analysis approaches in high throughput screening

Goktug AN et al. IntechOpen, 2013

General Stepwise Approach to Optimize a TR-FRET Assay for Characterizing the BRD/PROTAC/CRBN Ternary Complex

Lin W et al. ACS Pharmacol Transl Sci, 2021

GUItars: a GUI tool for analysis of high-throughput RNA interference screening data

Goktug AN et al. PLoS One, 2012

About the Director

Dr. Taosheng Chen is a Full Member at the Department of Chemical Biology & Therapeutics and the Director of the HTB Center. He received his BSc and MS degrees from Fudan University, China, and completed his Ph.D. studies from the University of Vermont and postdoctoral studies from the University of Virginia. Before joining St. Jude in 2006, Dr. Chen was a Senior Research Investigator at Bristol-Myers Squibb and a Research Scientist at SAIC-Frederick, National Cancer Institute. He serves on journal Editorial Boards and NIH study sections (chairperson of the DMP study section). In 2009, Dr. Chen published his first book, “A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery.” In 2017, he became one of the 39 inaugural Fellow Members of the Society for Laboratory Automation and Screening (SLAS). In addition to directing the HTB Center, his NIH-funded research laboratory studies the transcriptional regulation of drug responses.

Taosheng Chen Bio