The Center for Advanced Genome Engineering (CAGE) provides expertise and infrastructure for St. Jude investigators to educate, assist, expedite and perform established and emerging genome engineering technologies, including the CRISPR-Cas9 technology. The CAGE has 3 main objectives:
- Introduce user-defined missense mutations, knock-out, and knock-in mutations in human, mouse, rat and Drosophila cell lines as well as human pluripotent stem cells.
- Design and generate the materials and validated genotyping protocols to streamline the rapid production of knockout and knock-in preclinical animal models in collaboration with the existing St. Jude Transgenic/Gene Knockout Facility.
- Identify, validate and introduce newly emerging genome editing technologies and applications to the St. Jude community.