About the Tsai Lab

 Genome editing technologies have great potential for biomedical research and therapeutic applications. Our laboratory research centers on: understanding the safety of genome editors including unintended off-target activity and immunogenicity, protein engineering to improve properties of CRISPR-Cas genome editors, and developing therapeutic genome editing of human hematopoietic stem cells to treat diseases like sickle cell anemia.

Science Team

Our research summary

The research in our laboratory aims to advance genome editing techniques and technologies and use those approaches to treat human genetic diseases. Our highly collaborative research program focuses on developing fundamental genome editing technologies, addresses the safety of genome editing, and translates editing of hematopoietic stems cells as a possible treatment for sickle cell disease.

Specifically, our laboratory is working on protein engineering to improve the properties of CRISPR/Cas9 genome editors, including the overall activities of the editors and the specificity and precision of potential edits. Our work on fundamental genome editing technologies explores strategies for expanding the targeting range of gene editing while maintaining the activity of the editors in order to precisely change disease-causing mutations of interest.

Digital image of artificial intelligence human brain

As we explore technological advances to genome editors, our laboratory is simultaneously studying the safety of genome editing, particularly unintended off-target effects. As part of the NIH Somatic Cell Genome Editing Program, we are investigating the biological consequences of intended and unintended genome editing activities.  

Digital image of artificial intelligence human brain

Our laboratory also has a translational line of research that focuses on editing hematopoietic stems cells in order to introduce fetal hemoglobin to treat patients with sickle cell disease. This work involves disruption of a repressor-binding site in human hematopoietic stems cells. By doing so, we can achieve about a 30% induction of fetal hemoglobin in the erythroid progeny of editing human HSCs mobilized from patients with sickle cell disease. This level of fetal hemoglobin is predicted to be sufficient to achieve therapeutic impact. We are collaborating with other investigators and teams at St. Jude to advance this approach to a clinical trial. 

Selected Publications

About Shengdar Q. Tsai

Dr. Tsai is an Assistant Member in the Department of Hematology at St. Jude Children’s Research Hospital. His research lab focuses on developing genome editing technologies for therapeutics. His group has recently developed CHANGE-seq, a state-of-the-art, sensitive, unbiased, high-throughput method for defining the genome-wide activity of genome editors. Dr. Tsai completed a postdoctoral fellowship at Massachusetts General Hospital & Harvard Medical School, Ph.D. in Functional Genomics and M.S. in Bioinformatics from North Carolina State University. In 2020, he was chosen as one of the American Society for Gene and Cell Therapy Outstanding New Investigators. He is a co-chair of the Steering Committee of the NIH Somatic Cell Genome Editing Consortium.

Shengdar Q. Tsai

The team

 Creative and translational group of scientists with expertise in genome editing, cell and molecular biology, and genomics

Explore Career Opportunities in the Tsai Lab

We are currently recruiting!

Contact us

Shengdar Q. Tsai, PhD
Experimental Hematology
MS355

St. Jude Children’s Research Hospital

262 Danny Thomas Place
Memphis, TN, 38105-3678 USA
901-595-0407 Shengdar.Tsai@stjude.org

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262 Danny Thomas Place
Memphis, TN, 38105-3678 USA
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