Center for Advanced Genome Engineering (CAGE)

The Center for Advanced Genome Engineering (CAGE) provides expertise and infrastructure for St. Jude investigators to educate, assist, expedite and perform established and emerging genome engineering technologies, including the CRISPR-Cas9 technology. The CAGE has 3 main objectives:

  1. Introduce user-defined missense mutations, knock-out, and knock-in mutations in human, mouse, rat and Drosophila cell lines as well as human pluriportent stem cells.
  2. Design and generate the materials and validated genotyping protocols to streamline the rapid production of knockout and knock-in preclinical animal models in collaboration with the existing St. Jude Transgenic/Gene Knockout Facility.
  3. Identify, validate and introduce newly emerging genome editing technologies and applications to the St. Jude community.